PMID- 36764522
OWN - NLM
STAT- MEDLINE
DCOM- 20230329
LR  - 20230331
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 299
IP  - 3
DP  - 2023 Mar
TI  - Functional characterization of Vip3Aa from Bacillus thuringiensis reveals the 
      contributions of specific domains to its insecticidal activity.
PG  - 103000
LID - S0021-9258(23)00132-1 [pii]
LID - 10.1016/j.jbc.2023.103000 [doi]
LID - 103000
AB  - Microbially derived, protein-based biopesticides offer a more sustainable pest 
      management alternative to synthetic pesticides. Vegetative insecticidal proteins 
      (Vip3), multidomain proteins secreted by Bacillus thuringiensis, represent a 
      second-generation insecticidal toxin that has been preliminarily used in 
      transgenic crops. However, the molecular mechanism underlying Vip3's toxicity is 
      poorly understood. Here, we determine the distinct functions and contributions of 
      the domains of the Vip3Aa protein to its toxicity against Spodoptera frugiperda 
      larvae. We demonstrate that Vip3Aa domains II and III (DII-DIII) bind the midgut 
      epithelium, while DI is essential for Vip3Aa's stability and toxicity inside the 
      protease-enriched host insect midgut. DI-DIII can be activated by midgut 
      proteases and exhibits cytotoxicity similar to full-length Vip3Aa. In addition, 
      we determine that DV can bind the peritrophic matrix via its glycan-binding 
      activity, which contributes to Vip3Aa insecticidal activity. In summary, this 
      study provides multiple insights into Vip3Aa's mode-of-action which should 
      significantly facilitate the clarification of its insecticidal mechanism and its 
      further rational development.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Jiang, Kun
AU  - Jiang K
AD  - State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 
      China.
FAU - Chen, Zhe
AU  - Chen Z
AD  - State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 
      China.
FAU - Zang, Yuanrong
AU  - Zang Y
AD  - State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 
      China.
FAU - Shi, Yiting
AU  - Shi Y
AD  - School of Life Sciences, Shandong University, Qingdao, China; Taishan College, 
      Shandong University, Jinan, China.
FAU - Shang, Chengbin
AU  - Shang C
AD  - School of Life Sciences, Shandong University, Qingdao, China.
FAU - Jiao, Xuyao
AU  - Jiao X
AD  - State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 
      China.
FAU - Cai, Jun
AU  - Cai J
AD  - Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 
      China.
FAU - Gao, Xiang
AU  - Gao X
AD  - State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 
      China. Electronic address: xgao@email.sdu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230209
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Insecticides)
RN  - 0 (Bacterial Proteins)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - Animals
MH  - *Bacillus thuringiensis/chemistry
MH  - *Insecticides/chemistry
MH  - Bacterial Proteins/metabolism
MH  - Peptide Hydrolases/metabolism
MH  - Larva/metabolism
MH  - Spodoptera/metabolism
MH  - Pest Control, Biological
PMC - PMC10017365
OTO - NOTNLM
OT  - activation mechanism
OT  - bacterial pesticidal protein
OT  - insecticidal processes
OT  - molecular insecticidal mechanism
OT  - peritrophic matrix
COIS- Conflict of interest The authors declare that they have no conflicts of interest 
      with the contents of this article.
EDAT- 2023/02/11 06:00
MHDA- 2023/03/29 06:05
PMCR- 2023/02/09
CRDT- 2023/02/10 19:27
PHST- 2022/12/02 00:00 [received]
PHST- 2023/01/31 00:00 [revised]
PHST- 2023/02/02 00:00 [accepted]
PHST- 2023/03/29 06:05 [medline]
PHST- 2023/02/11 06:00 [pubmed]
PHST- 2023/02/10 19:27 [entrez]
PHST- 2023/02/09 00:00 [pmc-release]
AID - S0021-9258(23)00132-1 [pii]
AID - 103000 [pii]
AID - 10.1016/j.jbc.2023.103000 [doi]
PST - ppublish
SO  - J Biol Chem. 2023 Mar;299(3):103000. doi: 10.1016/j.jbc.2023.103000. Epub 2023 
      Feb 9.