PMID- 3676593
OWN - NLM
STAT- MEDLINE
DCOM- 19871224
LR  - 20190510
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 92
IP  - 2
DP  - 1987 Oct
TI  - Studies on the induction of pharmacological responses to des-Arg9-bradykinin in 
      vitro and in vivo.
PG  - 257-64
AB  - 1 The mechanisms by which agents modulate the induction of kinin B1-receptors 
      were investigated by studying the effects of kinins in vitro, by use of the 
      rabbit isolated aorta, and in vivo by measuring the blood pressure of 
      anaesthetized rabbits. 2 The contractile response of the rabbit isolated aorta to 
      kinins increased in a time-dependent manner in vitro. This effect was abolished 
      by continuous exposure to the protein synthesis inhibitor cycloheximide (71 
      microM). 3 Several substances were found to increase specifically the rate of 
      sensitization to des-Arg9-bradykinin (des-Arg9-Bk), when applied continuously in 
      vitro to tissues isolated from normal animals: bacterial lipopolysaccharide (LPS; 
      1 micrograms ml-1), muramyl-dipeptide (MDP; 2 micrograms ml-1), phorbol myristate 
      acetate (PMA; 320 nM), epidermal growth factor (EGF; 100 ng ml-1) and endothelial 
      cell growth factor (150 micrograms ml-1). 4 The protease inhibitors 
      phenylmethylsulphonyl fluoride and aprotinin, a non-adjuvant isomer of MDP, 
      rabbit purified leukocyte interferon, fibroblast growth factor and the 
      chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) did not 
      have this effect. 5. It has been demonstrated that LPS induces B1-receptors in 
      rabbits enabling des-Arg9-Bk to act as a hypotensive agent. In these experiments 
      neutropenia induced by nitrogen mustard, did not prevent the in vivo effect of 
      LPS. MDP (300 micrograms) and PMA (100 micrograms) were also found to induce a 
      state of responsiveness to des-Arg9-Bk in vivo. FMLP (1 mg i.v.) induced a 
      temporary decrease in blood neutrophil counts but had no effect on the induction 
      of responses to des-Arg9-Bk. 6. The development of responses mediated by the 
      B,-receptor in the two experimental systems seems to be unrelated to the 
      activation of neutrophil leukocytes, but may be related to the activation of 
      tissue macrophages. Approximately 3% of cultured adherent cells derived from 
      rabbit aorta strips following protease digestion were stained for non-specific 
      esterase, supporting such a possibility.
FAU - Bouthillier, J
AU  - Bouthillier J
AD  - Unite de Recherche Inflammation et Immuno-Rhumatologie, Centre Hospitalier de 
      l'Universite Laval, Quebec, Canada.
FAU - Deblois, D
AU  - Deblois D
FAU - Marceau, F
AU  - Marceau F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Kinins)
RN  - 15958-92-6 (bradykinin, des-Arg(9)-)
RN  - S8TIM42R2W (Bradykinin)
SB  - IM
MH  - Anesthesia
MH  - Animals
MH  - Aorta, Thoracic/drug effects
MH  - Blood Cell Count
MH  - Blood Pressure/drug effects
MH  - Bradykinin/*analogs & derivatives/pharmacology
MH  - Female
MH  - In Vitro Techniques
MH  - Kinins/pharmacology
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Muscle, Smooth, Vascular/drug effects
MH  - Rabbits
PMC - PMC1853641
EDAT- 1987/10/01 00:00
MHDA- 1987/10/01 00:01
PMCR- 1988/10/01
CRDT- 1987/10/01 00:00
PHST- 1987/10/01 00:00 [pubmed]
PHST- 1987/10/01 00:01 [medline]
PHST- 1987/10/01 00:00 [entrez]
PHST- 1988/10/01 00:00 [pmc-release]
AID - 10.1111/j.1476-5381.1987.tb11319.x [doi]
PST - ppublish
SO  - Br J Pharmacol. 1987 Oct;92(2):257-64. doi: 10.1111/j.1476-5381.1987.tb11319.x.