PMID- 36768757
OWN - NLM
STAT- MEDLINE
DCOM- 20230214
LR  - 20230214
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 3
DP  - 2023 Jan 26
TI  - A Study on Symbiotic Systems of Cicadas Provides New Insights into Distribution 
      of Microbial Symbionts and Improves Fluorescence In Situ Hybridization Technique.
LID - 10.3390/ijms24032434 [doi]
LID - 2434
AB  - Nutritional symbionts of sap-sucking auchenorrhynchan insects of Hemiptera are 
      usually confined to the bacteriomes and/or fat bodies. Knowledge is limited about 
      the distribution of microbial symbionts in other organs. We investigated the 
      distribution of obligate symbionts in the salivary glands, gut tissues, 
      reproductive organs, bacteriomes, and fat bodies of two cicada species, Karenia 
      caelatata and Tanna sp., using integrated methods, including a modified 
      fluorescence in situ hybridization (FISH) technique, which can greatly enhance 
      the FISH signal intensity of related symbionts. We revealed that Candidatus 
      Sulcia muelleri (Sulcia) and a yeast-like fungal symbiont (YLS) were harbored in 
      the bacteriomes and fat bodies, respectively. Both of Sulcia and YLS can be 
      transmitted to the offspring via ovaries, forming a "symbiont ball" in each egg. 
      Neither Sulcia nor YLS were harbored in the salivary glands, gut tissues and 
      testes. Phylogenetic trees of both Sulcia and cicadas confirm that K. caelatata 
      is a member of the tribe Dundubiini, and the tribe Leptopsaltriini that comprises 
      Ta. sp. is not monophyletic. YLS of K. caelatata is embedded inside the lineage 
      of YLS of Dundubiini, whereas YLS of Ta. sp. is closely related to the clade 
      comprising both cicada-parasitizing fungi Ophiocordyceps and YLS of Mogannia 
      conica and Meimuna mongolica, suggesting an evolutionary replacement of YLS in 
      Ta. sp. from an Ophiocordyceps fungus to another Ophiocordyceps fungus. Our 
      results provide new insights into the symbiosis between Cicadidae and related 
      symbionts. Modification through the addition of helpers and heat shock greatly 
      enhanced the FISH signal intensity of YLS, which may provide guidelines for 
      enhancement of the hybridization signal intensity of other symbiont(s) in the 
      FISH experiments.
FAU - Huang, Zhi
AU  - Huang Z
AD  - Key Laboratory of Plant Protection Resources and Pest Management of the Ministry 
      of Education, College of Plant Protection, Northwest A&F University, Xianyang 
      712100, China.
AD  - College of Forestry, Northwest A&F University, Xianyang 712100, China.
FAU - Zhou, Jinrui
AU  - Zhou J
AD  - Key Laboratory of Plant Protection Resources and Pest Management of the Ministry 
      of Education, College of Plant Protection, Northwest A&F University, Xianyang 
      712100, China.
FAU - Zhang, Zhijun
AU  - Zhang Z
AD  - College of Forestry, Northwest A&F University, Xianyang 712100, China.
FAU - He, Hong
AU  - He H
AUID- ORCID: 0000-0001-8267-3855
AD  - College of Forestry, Northwest A&F University, Xianyang 712100, China.
FAU - Wei, Cong
AU  - Wei C
AUID- ORCID: 0000-0002-1492-7240
AD  - Key Laboratory of Plant Protection Resources and Pest Management of the Ministry 
      of Education, College of Plant Protection, Northwest A&F University, Xianyang 
      712100, China.
LA  - eng
GR  - 32270496 and 32070476/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230126
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Animals
MH  - *Hemiptera/genetics
MH  - Phylogeny
MH  - Symbiosis
MH  - In Situ Hybridization, Fluorescence
MH  - Biological Evolution
MH  - *Ascomycota
PMC - PMC9917331
OTO - NOTNLM
OT  - Sulcia
OT  - YLS
OT  - evolution
OT  - sap-feeding insects
OT  - symbiosis
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/12 06:00
MHDA- 2023/02/15 06:00
PMCR- 2023/01/26
CRDT- 2023/02/11 01:21
PHST- 2022/12/24 00:00 [received]
PHST- 2023/01/18 00:00 [revised]
PHST- 2023/01/22 00:00 [accepted]
PHST- 2023/02/11 01:21 [entrez]
PHST- 2023/02/12 06:00 [pubmed]
PHST- 2023/02/15 06:00 [medline]
PHST- 2023/01/26 00:00 [pmc-release]
AID - ijms24032434 [pii]
AID - ijms-24-02434 [pii]
AID - 10.3390/ijms24032434 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Jan 26;24(3):2434. doi: 10.3390/ijms24032434.