PMID- 36768868
OWN - NLM
STAT- MEDLINE
DCOM- 20230214
LR  - 20230214
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 3
DP  - 2023 Jan 29
TI  - Characterization of Stress Granule Protein Turnover in Neuronal Progenitor Cells 
      Using Correlative STED and NanoSIMS Imaging.
LID - 10.3390/ijms24032546 [doi]
LID - 2546
AB  - Stress granules (SGs) are stress-induced biomolecular condensates which originate 
      primarily from inactivated RNA translation machinery and translation initiation 
      factors. SG formation is an important defensive mechanism for cell survival, 
      while its dysfunction has been linked to neurodegenerative diseases. However, the 
      molecular mechanisms of SG assembly and disassembly, as well as their impacts on 
      cellular recovery, are not fully understood. More thorough investigations into 
      the molecular dynamics of SG pathways are required to understand the 
      pathophysiological roles of SGs in cellular systems. Here, we characterize the SG 
      and cytoplasmic protein turnover in neuronal progenitor cells (NPCs) under 
      stressed and non-stressed conditions using correlative STED and NanoSIMS imaging. 
      We incubate NPCs with isotopically labelled ((15)N) leucine and stress them with 
      the ER stressor thapsigargin (TG). A correlation of STED and NanoSIMS allows the 
      localization of individual SGs (using STED), and their protein turnover can then 
      be extracted based on the (15)N/(14)N ratio (using NanoSIMS). We found that 
      TG-induced SGs, which are highly dynamic domains, recruit their constituents 
      predominantly from the cytoplasm. Moreover, ER stress impairs the total cellular 
      protein turnover regimen, and this impairment is not restored after the commonly 
      proceeded stress recovery period.
FAU - Rabasco, Stefania
AU  - Rabasco S
AUID- ORCID: 0000-0001-5260-2909
AD  - Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 
      96 Gothenburg, Sweden.
FAU - Lork, Alicia A
AU  - Lork AA
AD  - Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 
      96 Gothenburg, Sweden.
FAU - Berlin, Emmanuel
AU  - Berlin E
AUID- ORCID: 0000-0002-8262-0662
AD  - Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 
      96 Gothenburg, Sweden.
FAU - Nguyen, Tho D K
AU  - Nguyen TDK
AUID- ORCID: 0000-0002-8306-6181
AD  - Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 
      96 Gothenburg, Sweden.
FAU - Ernst, Carl
AU  - Ernst C
AD  - Human Genetics, McGill University, Montreal, QC H4H1R3, Canada.
FAU - Locker, Nicolas
AU  - Locker N
AUID- ORCID: 0000-0002-0053-2897
AD  - Faculty of Health and Medical Sciences, School of Biosciences and Medicine, 
      University of Surrey, Guildford GU2 7XH, UK.
FAU - Ewing, Andrew G
AU  - Ewing AG
AUID- ORCID: 0000-0002-2084-0133
AD  - Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 
      96 Gothenburg, Sweden.
FAU - Phan, Nhu T N
AU  - Phan NTN
AD  - Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 
      96 Gothenburg, Sweden.
LA  - eng
GR  - 787534/European Research Council/International
GR  - 2017-04366 and 2017-05962/Swedish Research Council/
GR  - None/Knut and Alice Wallenberg Foundation/
GR  - 2020-00815/Swedish Research Council/
GR  - MR/R02426X/1/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20230129
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Humans
MH  - *Cytoplasmic Granules/metabolism
MH  - Stress Granules
MH  - Cytoplasm
MH  - *Neurodegenerative Diseases/metabolism
MH  - Stem Cells
MH  - Stress, Physiological
PMC - PMC9917160
OTO - NOTNLM
OT  - NanoSIMS
OT  - mass spectrometry imaging
OT  - neuronal progenitor cells
OT  - protein turnover
OT  - stress granules
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/12 06:00
MHDA- 2023/02/15 06:00
PMCR- 2023/01/29
CRDT- 2023/02/11 01:22
PHST- 2022/12/22 00:00 [received]
PHST- 2023/01/18 00:00 [revised]
PHST- 2023/01/23 00:00 [accepted]
PHST- 2023/02/11 01:22 [entrez]
PHST- 2023/02/12 06:00 [pubmed]
PHST- 2023/02/15 06:00 [medline]
PHST- 2023/01/29 00:00 [pmc-release]
AID - ijms24032546 [pii]
AID - ijms-24-02546 [pii]
AID - 10.3390/ijms24032546 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Jan 29;24(3):2546. doi: 10.3390/ijms24032546.