PMID- 36770751
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20230213
LR  - 20230214
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 28
IP  - 3
DP  - 2023 Jan 21
TI  - An Aluminum-Based Microfluidic Chip for Polymerase Chain Reaction Diagnosis.
LID - 10.3390/molecules28031085 [doi]
LID - 1085
AB  - Real-time polymerase chain reaction (real-time PCR) tests were successfully 
      conducted in an aluminum-based microfluidic chip developed in this work. The 
      reaction chamber was coated with silicone-modified epoxy resin to isolate the 
      reaction system from metal surfaces, preventing the metal ions from interfering 
      with the reaction process. The patterned aluminum substrate was bonded with a 
      hydroxylated glass mask using silicone sealant at room temperature. The effect of 
      thermal expansion was counteracted by the elasticity of cured silicone. With the 
      heating process closely monitored, real-time PCR testing in reaction chambers 
      proceeded smoothly, and the results show similar quantification cycle values to 
      those of traditional test sets. Scanning electron microscope (SEM) and atomic 
      force microscopy (AFM) images showed that the surface of the reaction chamber was 
      smoothly coated, illustrating the promising coating and isolating properties. 
      Energy-dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy 
      (XPS), and inductively coupled plasma-optical emission spectrometer (ICP-OES) 
      showed that no metal ions escaped from the metal to the chip surface. 
      Fourier-transform infrared spectroscopy (FTIR) was used to check the surface 
      chemical state before and after tests, and the unchanged infrared absorption 
      peaks indicated the unreacted, antifouling surface. The limit of detection (LOD) 
      of at least two copies can be obtained in this chip.
FAU - Yang, Siyu
AU  - Yang S
AUID- ORCID: 0000-0003-1155-2436
AD  - Division of Emerging Interdisciplinary Areas, The Hong Kong University of Science 
      and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, China.
FAU - Zhang, Ziyi
AU  - Zhang Z
AD  - Division of Emerging Interdisciplinary Areas, The Hong Kong University of Science 
      and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, China.
FAU - Xian, Qingyue
AU  - Xian Q
AD  - Division of Emerging Interdisciplinary Areas, The Hong Kong University of Science 
      and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, China.
FAU - Song, Qi
AU  - Song Q
AD  - Department of Physics, The Hong Kong University of Science and Technology, Clear 
      Water Bay, Kowloon, Hong Kong SAR 999077, China.
FAU - Liu, Yiteng
AU  - Liu Y
AUID- ORCID: 0000-0003-1497-4239
AD  - Division of Emerging Interdisciplinary Areas, The Hong Kong University of Science 
      and Technology, Clear Water Bay, Kowloon, Hong Kong SAR 999077, China.
FAU - Gao, Yibo
AU  - Gao Y
AD  - Department of Physics, The Hong Kong University of Science and Technology, Clear 
      Water Bay, Kowloon, Hong Kong SAR 999077, China.
FAU - Wen, Weijia
AU  - Wen W
AD  - Department of Physics, The Hong Kong University of Science and Technology, Clear 
      Water Bay, Kowloon, Hong Kong SAR 999077, China.
AD  - Thrust of Advanced Materials, The Hong Kong University of Science and Technology 
      (Guangzhou), Nansha, Guangzhou 511400, China.
AD  - HKUST Shenzhen-Hong Kong Collaborative Innovation Research Institute, Futian, 
      Shenzhen 518000, China.
LA  - eng
GR  - MRP/084/20/Hong Kong Innovation and Technology Fund/
GR  - 202011023000417/Key Technology Project of Shenzhen Science and Technology 
      Innovation Commission/
GR  - HZQB-KCZYB-2020083/Project of Hetao Shenzhen-Hong Kong Science and Technology 
      Innovation Cooperation Zone/
PT  - Journal Article
DEP - 20230121
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
SB  - IM
PMC - PMC9921548
OTO - NOTNLM
OT  - aluminum chips
OT  - diagnosis
OT  - microfluidic chips
OT  - nucleic acid diagnosis
OT  - portability
OT  - real-time polymerase chain reaction
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/12 06:00
MHDA- 2023/02/12 06:01
PMCR- 2023/01/21
CRDT- 2023/02/11 01:34
PHST- 2022/12/27 00:00 [received]
PHST- 2023/01/13 00:00 [revised]
PHST- 2023/01/18 00:00 [accepted]
PHST- 2023/02/11 01:34 [entrez]
PHST- 2023/02/12 06:00 [pubmed]
PHST- 2023/02/12 06:01 [medline]
PHST- 2023/01/21 00:00 [pmc-release]
AID - molecules28031085 [pii]
AID - molecules-28-01085 [pii]
AID - 10.3390/molecules28031085 [doi]
PST - epublish
SO  - Molecules. 2023 Jan 21;28(3):1085. doi: 10.3390/molecules28031085.