PMID- 36773757
OWN - NLM
STAT- MEDLINE
DCOM- 20230331
LR  - 20230331
IS  - 1873-2259 (Electronic)
IS  - 0168-9452 (Linking)
VI  - 330
DP  - 2023 May
TI  - OsCRY2 and OsFBO10 co-regulate photomorphogenesis and photoperiodic flowering in 
      indica rice.
PG  - 111631
LID - S0168-9452(23)00048-1 [pii]
LID - 10.1016/j.plantsci.2023.111631 [doi]
AB  - Cryptochromes (CRYs) are a class of photoreceptors that perceive 
      blue/ultraviolet-A light of the visible spectrum to mediate a vast number of 
      physiological responses in bacteria, fungi, animals and plants. In the present 
      study, we have characterized OsCRY2 in a photoperiod sensitive indica variety, 
      Basmati 370, by generating and analyzing overexpression (OE) and knock-down (KD) 
      transgenic lines. The OsCRY2(OE) lines displayed dwarfism as shown in their 
      reduced plant height and leaf length, attributed largely by an overall reduction 
      in their cell size. The OsCRY2(OE) lines flowered significantly earlier and 
      showed shorter and broader seeds with an overall reduced seed weight. The 
      OsCRY2(KD) lines showed contrasting phenotypes, such as increased plant height 
      and delayed flowering, however, decreased seed size and weight were also observed 
      in the KD lines, along with reduced spikelet fertility and high seed shattering 
      rate in mature panicles. Novel interactions were confirmed between OsCRY2 and 
      members of ZEITLUPE family of blue/ultraviolet-A light photoreceptors, encoded by 
      OsFBO8, OsFBO9 and OsFBO10 which are orthologous to ZEITLUPE (ZTL), LOV KELCH 
      PROTEIN2 (LKP2) and FLAVIN BINDING, KELCH REPEAT F-BOX1 (FKF1), respectively, of 
      Arabidopsis thaliana. Since FKF1 is known to play a role in regulating 
      photoperiodic flowering, OsFBO10 was chosen for further studies. OsCRY2 and 
      OsFBO10 interacted in the nucleus and cytoplasm of the cell and cross-regulated 
      the expression of each other. They were also found to regulate the expression of 
      several genes involved in photoperiodic flowering in rice. Both OsCRY2 and 
      OsFBO10 played a positive role in photomorphogenic responses in different light 
      conditions. The physical interaction of OsCRY2 with OsFBO10, their involvement in 
      common physiological and developmental pathways and their cross-regulation of 
      each other suggest that the two photoreceptors may regulate common developmental 
      pathways in plants, either jointly or redundantly.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Singh, Shipra
AU  - Singh S
AD  - Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular 
      Biology, University of Delhi, South Campus, New Delhi 110021, India.
FAU - Vergish, Satyam
AU  - Vergish S
AD  - Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular 
      Biology, University of Delhi, South Campus, New Delhi 110021, India.
FAU - Jain, Nitin
AU  - Jain N
AD  - Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular 
      Biology, University of Delhi, South Campus, New Delhi 110021, India.
FAU - Sharma, Arun Kumar
AU  - Sharma AK
AD  - Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular 
      Biology, University of Delhi, South Campus, New Delhi 110021, India.
FAU - Khurana, Paramjit
AU  - Khurana P
AD  - Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular 
      Biology, University of Delhi, South Campus, New Delhi 110021, India. Electronic 
      address: param@genomeindia.org.
FAU - Khurana, Jitendra P
AU  - Khurana JP
AD  - Interdisciplinary Centre for Plant Genomics & Department of Plant Molecular 
      Biology, University of Delhi, South Campus, New Delhi 110021, India.
LA  - eng
PT  - Journal Article
DEP - 20230210
PL  - Ireland
TA  - Plant Sci
JT  - Plant science : an international journal of experimental plant biology
JID - 9882015
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (ZTL protein, Arabidopsis)
RN  - 0 (FKF1 protein, Arabidopsis)
RN  - 0 (LKP2 protein, Arabidopsis)
SB  - IM
MH  - Photoperiod
MH  - *Oryza/genetics/metabolism
MH  - *Arabidopsis/genetics
MH  - *Arabidopsis Proteins/metabolism
MH  - Flowers/metabolism
MH  - Plants/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Light
OTO - NOTNLM
OT  - Light signaling
OT  - OsCRY2
OT  - OsFBO10
OT  - Photomorphogenesis
OT  - Photoperiodic flowering
OT  - Rice
COIS- Declaration of Competing Interest The authors have no conflict of interest to 
      declare.
EDAT- 2023/02/12 06:00
MHDA- 2023/03/31 06:42
CRDT- 2023/02/11 19:25
PHST- 2022/12/02 00:00 [received]
PHST- 2023/02/02 00:00 [revised]
PHST- 2023/02/04 00:00 [accepted]
PHST- 2023/03/31 06:42 [medline]
PHST- 2023/02/12 06:00 [pubmed]
PHST- 2023/02/11 19:25 [entrez]
AID - S0168-9452(23)00048-1 [pii]
AID - 10.1016/j.plantsci.2023.111631 [doi]
PST - ppublish
SO  - Plant Sci. 2023 May;330:111631. doi: 10.1016/j.plantsci.2023.111631. Epub 2023 
      Feb 10.