PMID- 36776968
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230214
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 14
DP  - 2023
TI  - The effect of pre-exercise alkalosis on lactate/pH regulation and mitochondrial 
      respiration following sprint-interval exercise in humans.
PG  - 1073407
LID - 10.3389/fphys.2023.1073407 [doi]
LID - 1073407
AB  - Purpose: The purpose of this study was to evaluate the effect of pre-exercise 
      alkalosis, induced via ingestion of sodium bicarbonate, on changes to lactate/pH 
      regulatory proteins and mitochondrial function induced by a sprint-interval 
      exercise session in humans. Methods: On two occasions separated by 1 week, eight 
      active men performed a 3 x 30-s all-out cycling test, interspersed with 20 min of 
      recovery, following either placebo (PLA) or sodium bicarbonate (BIC) ingestion. 
      Results: Blood bicarbonate and pH were elevated at all time points after 
      ingestion in BIC vs PLA (p < 0.05). The protein content of monocarboxylate 
      transporter 1 (MCT1) and basigin (CD147), at 6 h and 24 h post-exercise, and 
      sodium/hydrogen exchanger 1 (NHE1) 24 h post-exercise, were significantly greater 
      in BIC compared to PLA (p < 0.05), whereas monocarboxylate transporter 4 (MCT4), 
      sodium/bicarbonate cotransporter (NBC), and carbonic anhydrase isoform II (CAII) 
      content was unchanged. These increases in protein content in BIC vs. PLA after 
      acute sprint-interval exercise may be associated with altered physiological 
      responses to exercise, such as the higher blood pH and bicarbonate concentration 
      values, and lower exercise-induced oxidative stress observed during recovery (p < 
      0.05). Additionally, mitochondrial respiration decreased after 24 h of recovery 
      in the BIC condition only, with no changes in oxidative protein content in either 
      condition. Conclusion: These data demonstrate that metabolic alkalosis induces 
      post-exercise increases in several lactate/pH regulatory proteins, and reveal an 
      unexpected role for acidosis in mitigating the loss of mitochondrial respiration 
      caused by exercise in the short term.
CI  - Copyright (c) 2023 Thomas, Delfour-Peyrethon, Lambert, Granata, Hobbs, Hanon and 
      Bishop.
FAU - Thomas, Claire
AU  - Thomas C
AD  - LBEPS, Univ Evry, IRBA, University Paris Saclay, Evry, France.
AD  - French Institute of Sport (INSEP), Research Department, Laboratory Sport, 
      Expertise, and Performance, Paris, France.
FAU - Delfour-Peyrethon, Remi
AU  - Delfour-Peyrethon R
AD  - French Institute of Sport (INSEP), Research Department, Laboratory Sport, 
      Expertise, and Performance, Paris, France.
AD  - Institute for Health and Sport (iHeS), Victoria University, Melbourne, VIC, 
      Australia.
FAU - Lambert, Karen
AU  - Lambert K
AD  - PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214, Montpellier, 
      France.
FAU - Granata, Cesare
AU  - Granata C
AD  - French Institute of Sport (INSEP), Research Department, Laboratory Sport, 
      Expertise, and Performance, Paris, France.
AD  - Department of Diabetes, Central Clinical School, Monash University, Melbourne, 
      VIC, Australia.
AD  - Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for 
      Diabetes Research at Heinrich Heine University Dusseldorf, Dusseldorf, Germany.
AD  - German Center for Diabetes Research, Partner Dusseldorf, Munchen-Neuherberg, 
      Germany.
FAU - Hobbs, Thomas
AU  - Hobbs T
AD  - LBEPS, Univ Evry, IRBA, University Paris Saclay, Evry, France.
FAU - Hanon, Christine
AU  - Hanon C
AD  - French Institute of Sport (INSEP), Research Department, Laboratory Sport, 
      Expertise, and Performance, Paris, France.
AD  - French Athletics Federation, Paris, France.
FAU - Bishop, David J
AU  - Bishop DJ
AD  - Institute for Health and Sport (iHeS), Victoria University, Melbourne, VIC, 
      Australia.
LA  - eng
PT  - Journal Article
DEP - 20230127
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC9911540
OTO - NOTNLM
OT  - MCT4
OT  - NBC
OT  - lactate
OT  - lactate transport
OT  - mct1
OT  - metabolic acidosis
OT  - mitochondrial function
OT  - sodium bicarbonate
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest. The reviewer DP declared a past co-authorship with the 
      author CG to the handling editor.
EDAT- 2023/02/14 06:00
MHDA- 2023/02/14 06:01
PMCR- 2023/01/27
CRDT- 2023/02/13 03:28
PHST- 2022/10/18 00:00 [received]
PHST- 2023/01/11 00:00 [accepted]
PHST- 2023/02/13 03:28 [entrez]
PHST- 2023/02/14 06:00 [pubmed]
PHST- 2023/02/14 06:01 [medline]
PHST- 2023/01/27 00:00 [pmc-release]
AID - 1073407 [pii]
AID - 10.3389/fphys.2023.1073407 [doi]
PST - epublish
SO  - Front Physiol. 2023 Jan 27;14:1073407. doi: 10.3389/fphys.2023.1073407. 
      eCollection 2023.