PMID- 36781245
OWN - NLM
STAT- MEDLINE
DCOM- 20230215
LR  - 20230215
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1247
DP  - 2023 Mar 22
TI  - Advantages of aggregation-induced luminescence microspheres compared with 
      fluorescent microspheres in immunochromatography assay with sandwich format.
PG  - 340869
LID - S0003-2670(23)00090-9 [pii]
LID - 10.1016/j.aca.2023.340869 [doi]
AB  - Organic fluorescein dye-embedded fluorescent microspheres (FMs) are currently the 
      most established commercially fluorescent markers, and they have been widely used 
      to improve the sensitivity of immunochromatography assay (ICA). However, these 
      FMs have natural defects, such as the aggregation-caused quenching effect and 
      small Stokes shift, which are not conducive to improving the detection 
      performance of ICA. Herein, two green emitted FMs, namely aggregation-induced 
      emission FMs (AIEFMs) and fluorescein isothiocyanate FMs (FITCFMs), were prepared 
      by swelling the AIE luminogens and FITC dyes into the carboxyl group-modified 
      polystyrene microspheres. The average diameters of AIEFMs and FITCFMs were 350 
      and 450 nm, respectively. Compared with FITCFMs, the AIEFMs exhibited stronger 
      fluorescence intensity and a larger Stokes shift. These two FMs were used as the 
      labeling markers of ICA for procalcitonin (PCT) detection with the sandwich 
      format. Among them, AIEFM-ICA showed dynamic linear detection of PCT from 
      7.6 pg mL(-1) to 125 ng mL(-1) with the limit of detection (LOD) at 
      3.8 pg mL(-1). These values were remarkably superior to those of FITCFM-ICA 
      (linear range from 61 pg mL(-1) to 62.5 ng mL(-1) and LOD value at 60 pg mL(-1)). 
      Furthermore, the average recoveries of the intra- and inter-assays of AIEFM-ICA 
      ranged from 86% to 112%, with coefficients of variation ranging from 1.2% to 
      8.8%, indicating accuracy and precision for PCT quantitative detection. 
      Additionally, the reliability of the developed AIEFM-ICA was further assessed by 
      analyzing 30 real serum samples from systemic inflammatory response by infectious 
      diseases, and the results showed good agreement with the chemiluminescence 
      immunoassay. In conclusion, compared with traditional FITCFMs, green emitted 
      AIEFMs as a novel fluorescent label, exhibits greater potential to enhance the 
      detection performance of the ICA platform.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Wei, Xiaxia
AU  - Wei X
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, China; School of Food Science and Technology, Nanchang 
      University, Nanchang, 330031, China.
FAU - Chen, Xirui
AU  - Chen X
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, China; School of Food Science and Technology, Nanchang 
      University, Nanchang, 330031, China.
FAU - Liu, Zilong
AU  - Liu Z
AD  - School of Food Science and Engineering, Hainan University, Haikou, 570228, China.
FAU - Xu, Ge
AU  - Xu G
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, China; School of Food Science and Technology, Nanchang 
      University, Nanchang, 330031, China.
FAU - Chen, Guoxin
AU  - Chen G
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, China; School of Food Science and Technology, Nanchang 
      University, Nanchang, 330031, China.
FAU - Lin, Xiangkai
AU  - Lin X
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, China; School of Food Science and Technology, Nanchang 
      University, Nanchang, 330031, China.
FAU - Zhan, Wenchao
AU  - Zhan W
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, China; School of Food Science and Technology, Nanchang 
      University, Nanchang, 330031, China.
FAU - Huang, Xiaolin
AU  - Huang X
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, China; School of Food Science and Technology, Nanchang 
      University, Nanchang, 330031, China. Electronic address: hxl19880503@163.com.
FAU - Xiong, Yonghua
AU  - Xiong Y
AD  - State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang, 330047, China; School of Food Science and Technology, Nanchang 
      University, Nanchang, 330031, China; Jiangxi-OAI Joint Research Institute, 
      Nanchang University, Nanchang, 330047, China. Electronic address: 
      yhxiongchen@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230121
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Coloring Agents)
RN  - 0 (Fluoresceins)
SB  - IM
MH  - *Luminescence
MH  - Microspheres
MH  - Reproducibility of Results
MH  - *Coloring Agents
MH  - Chromatography, Affinity/methods
MH  - Fluoresceins
MH  - Immunoassay/methods
OTO - NOTNLM
OT  - Aggregation-induced luminescence microspheres
OT  - Detection performance
OT  - Immunochromatography assay
OT  - Organic fluorescein dye-embedded microspheres
OT  - Procalcitonin
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/02/14 06:00
MHDA- 2023/02/16 06:00
CRDT- 2023/02/13 20:54
PHST- 2022/12/15 00:00 [received]
PHST- 2023/01/20 00:00 [accepted]
PHST- 2023/02/13 20:54 [entrez]
PHST- 2023/02/14 06:00 [pubmed]
PHST- 2023/02/16 06:00 [medline]
AID - S0003-2670(23)00090-9 [pii]
AID - 10.1016/j.aca.2023.340869 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2023 Mar 22;1247:340869. doi: 10.1016/j.aca.2023.340869. Epub 
      2023 Jan 21.