PMID- 36785490
OWN - NLM
STAT- MEDLINE
DCOM- 20230810
LR  - 20230811
IS  - 1742-481X (Electronic)
IS  - 1742-4801 (Print)
IS  - 1742-4801 (Linking)
VI  - 20
IP  - 7
DP  - 2023 Sep
TI  - Role of macrophage polarisation in skin wound healing.
PG  - 2551-2562
LID - 10.1111/iwj.14119 [doi]
AB  - Skin wound healing is a complex pathophysiological change that is driven by 
      macrophages and their secreted related factors. Depending on the stimuli, 
      macrophages can be polarised into two subtypes of macrophages with completely 
      different phenotypes and functions, namely M1 and M2. The aim of this study was 
      to explore the role of M1 and M2 macrophages in skin healing in order to develop 
      new drugs for the treatment of refractory wounds. Primary bone marrow-derived 
      macrophages (BMDMs) were isolated from rats and expanded in vitro using 
      macrophage colony stimulating factor. In addition, the BMDMs were polarised into 
      the M1 and M2 subtypes using lipopolysaccharides (LPS) and interleukin-4 (IL-4), 
      respectively. Cytokine levels in the culture supernatants were measured by an 
      enzyme linked immunosorbent assay. Epidermal wounds were made on the dorsal 
      surface of rats, and treated with M1 or M2 cell suspensions or phosphate buffered 
      saline. Wound healing was recorded on days 1, 3, 7, 10, and 14 after stamping, 
      and the wound healing rate was measured by haematoxylin-eosin and Masson 
      staining. A total of 3 to 4 x 10(7) bone marrow cells were extracted from each 
      rat femur. The BMDM culture had 87.1% CD45(+) cells, 89.2% CD68(+) cells, and 
      86.5% CD45(+) CD68(+) cells. Furthermore, IL-12 (P < .05) and IL-10 (P >/= .05) 
      levels, respectively, increased and decreased in the culture supernatants of the 
      M1 cells after LPS stimulation compared with those in the M0 (unstimulated) 
      group. Likewise, IL-4 stimulation led to a significant increase in IL-10 levels 
      (P < .01) in the conditioned media of M2 cells, while that of IL-12 decreased 
      slightly (P >/= .05). In the rat model, the infusion of M2 cells accelerated wound 
      healing and tissue regeneration, whereas the M1 cells delayed the recruitment of 
      inflammatory cells, granulation growth, and collagen deposition, which impaired 
      wound healing. Macrophage polarisation and activation are critical for skin wound 
      healing. While exogenous M1 cell infusion delayed wound healing, the M2 cells 
      promoted wound healing in a rat model.
CI  - (c) 2023 The Authors. International Wound Journal published by Medicalhelplines.com 
      Inc and John Wiley & Sons Ltd.
FAU - Gao, Xuejun
AU  - Gao X
AD  - Department of Thyroid Surgery, The Affiliated Hospital of Qingdao University, 
      Qingdao, China.
FAU - Lu, Chao
AU  - Lu C
AD  - Department of Hand and Foot Surgery, The Affiliated Hospital of Qingdao 
      University, Qingdao, China.
FAU - Miao, Yuanxin
AU  - Miao Y
AD  - Department of Plastic Surgery, The Affiliated Hospital of Qingdao University, 
      Qingdao, China.
FAU - Ren, Jizhen
AU  - Ren J
AD  - Department of Plastic Surgery, The Affiliated Hospital of Qingdao University, 
      Qingdao, China.
FAU - Cai, Xia
AU  - Cai X
AUID- ORCID: 0000-0001-7885-1145
AD  - Department of Plastic Surgery, The Affiliated Hospital of Qingdao University, 
      Qingdao, China.
LA  - eng
PT  - Journal Article
DEP - 20230213
PL  - England
TA  - Int Wound J
JT  - International wound journal
JID - 101230907
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 0 (Lipopolysaccharides)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Interleukin-10
MH  - *Interleukin-4
MH  - Lipopolysaccharides
MH  - Interleukin-12
MH  - Macrophages
MH  - Wound Healing
PMC - PMC10410349
OTO - NOTNLM
OT  - bone marrow-derived macrophages
OT  - immune cell
OT  - macrophage
OT  - wound healing
COIS- The authors declare that they have no conflict of interest.
EDAT- 2023/02/15 06:00
MHDA- 2023/08/10 06:43
PMCR- 2023/02/13
CRDT- 2023/02/14 01:27
PHST- 2023/01/29 00:00 [revised]
PHST- 2022/12/06 00:00 [received]
PHST- 2023/01/30 00:00 [accepted]
PHST- 2023/08/10 06:43 [medline]
PHST- 2023/02/15 06:00 [pubmed]
PHST- 2023/02/14 01:27 [entrez]
PHST- 2023/02/13 00:00 [pmc-release]
AID - IWJ14119 [pii]
AID - 10.1111/iwj.14119 [doi]
PST - ppublish
SO  - Int Wound J. 2023 Sep;20(7):2551-2562. doi: 10.1111/iwj.14119. Epub 2023 Feb 13.