PMID- 36786319
OWN - NLM
STAT- MEDLINE
DCOM- 20230309
LR  - 20240215
IS  - 1943-4936 (Electronic)
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 35
IP  - 2
DP  - 2023 Mar
TI  - Development and field evaluation of an ELISA to differentiate Anaplasma 
      marginale-infected from A. centrale-vaccinated cattle.
PG  - 204-208
LID - 10.1177/10406387231152472 [doi]
AB  - Immunization of calves with Anaplasma centrale is used to prevent acute 
      anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is 
      detected through serologic tests based primarily on A. marginale recombinant 
      major surface protein 5 (MSP5m) because it has 91% identity with MSP5 from A. 
      centrale (MSP5c). We developed a displacement, double-antigen, sandwich ELISA 
      (ddasELISA) to detect antibodies against A. marginale or A. centrale. For 
      ddasELISA validation, we analyzed serum samples positive for antibodies against 
      Anaplasma spp. from cattle naturally infected with A. marginale (n = 300) or 
      vaccinated with A. centrale (n = 255). Species-specific nested PCR (nPCR) assays 
      were used to confirm infection. The optical density (OD) values obtained from 
      antibodies directed at unique epitopes of A. marginale (OD(Am)) or A. centrale 
      (OD(Ac)) were used in the formula OD(Am)/OD(Ac). If the derived ratio was >0.38, 
      the serum sample was considered positive for antibodies against A. marginale, 
      with 98.9% sensitivity and 98.0% specificity. In a field evaluation, we analyzed 
      702 Anaplasma spp. antibody-positive serum samples from 34 herds by ddasELISA and 
      nPCR; 571 were classified by ddasELISA as A. marginale-infected or A. 
      centrale-vaccinated, with 84% agreement (kappa = 0.70) between ddasELISA and nPCR. 
      Our results indicate that ddasELISA could be used as a cost-effective alternative 
      to molecular techniques to confirm infection with A. marginale in countries in 
      which prevention is based on vaccination with A. centrale.
FAU - Bellezze, Julio
AU  - Bellezze J
AD  - Laboratorio de Inmunologia y Parasitologia, Instituto de Investigacion de la 
      Cadena Lactea (IdICaL, INTA-CONICET), Rafaela, Santa Fe, Argentina.
FAU - Thompson, Carolina S
AU  - Thompson CS
AD  - Laboratorio de Inmunologia y Parasitologia, Instituto de Investigacion de la 
      Cadena Lactea (IdICaL, INTA-CONICET), Rafaela, Santa Fe, Argentina.
FAU - Bosio, Anabela S
AU  - Bosio AS
AD  - Laboratorio de Inmunologia y Parasitologia, Instituto de Investigacion de la 
      Cadena Lactea (IdICaL, INTA-CONICET), Rafaela, Santa Fe, Argentina.
FAU - Torioni, Susana M
AU  - Torioni SM
AD  - Laboratorio de Inmunologia y Parasitologia, Instituto de Investigacion de la 
      Cadena Lactea (IdICaL, INTA-CONICET), Rafaela, Santa Fe, Argentina.
FAU - Primo, Maria E
AU  - Primo ME
AUID- ORCID: 0000-0002-6873-8867
AD  - Laboratorio de Inmunologia y Parasitologia, Instituto de Investigacion de la 
      Cadena Lactea (IdICaL, INTA-CONICET), Rafaela, Santa Fe, Argentina.
LA  - eng
PT  - Journal Article
DEP - 20230214
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Cattle
MH  - Animals
MH  - *Anaplasma marginale
MH  - *Anaplasmosis/diagnosis/prevention & control
MH  - Anaplasma
MH  - *Anaplasma centrale
MH  - Enzyme-Linked Immunosorbent Assay/veterinary/methods
MH  - Recombinant Proteins
MH  - *Cattle Diseases/diagnosis/prevention & control
PMC - PMC9999405
OTO - NOTNLM
OT  - Anaplasma centrale
OT  - Anaplasma marginale
OT  - ELISA
OT  - species-specific antibodies
COIS- The authors declared no potential conflicts of interest with respect to the 
      research, authorship, and/or publication of this article.
EDAT- 2023/02/15 06:00
MHDA- 2023/03/10 06:00
PMCR- 2024/02/14
CRDT- 2023/02/14 06:13
PHST- 2023/02/15 06:00 [pubmed]
PHST- 2023/03/10 06:00 [medline]
PHST- 2023/02/14 06:13 [entrez]
PHST- 2024/02/14 00:00 [pmc-release]
AID - 10.1177_10406387231152472 [pii]
AID - 10.1177/10406387231152472 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2023 Mar;35(2):204-208. doi: 10.1177/10406387231152472. Epub 
      2023 Feb 14.