PMID- 36790930
OWN - NLM
STAT- MEDLINE
DCOM- 20231005
LR  - 20231005
IS  - 2211-1247 (Electronic)
VI  - 42
IP  - 2
DP  - 2023 Feb 28
TI  - Actin-dependent astrocytic infiltration is a key step for axon defasciculation 
      during remodeling.
PG  - 112117
LID - S2211-1247(23)00128-6 [pii]
LID - 10.1016/j.celrep.2023.112117 [doi]
LID - 112117
AB  - Astrocytes are essential for synapse formation, maturation, and plasticity; 
      however, their function during developmental neuronal remodeling is largely 
      unknown. To identify astrocytic molecules required for axon pruning of mushroom 
      body (MB) gamma neurons in Drosophila, we profiled astrocytes before (larva) and 
      after (adult) remodeling. Focusing on genes enriched in larval astrocytes, we 
      identified 12 astrocytic genes that are required for axon pruning, including the 
      F-actin regulators Actin-related protein 2/3 complex, subunit 1 (Arpc1) and 
      formin3 (form3). Interestingly, perturbing astrocytic actin dynamics does not 
      affect their gross morphology, migration, or transforming growth factor beta (TGF-beta) 
      secretion. In contrast, actin dynamics is required for astrocyte infiltration 
      into the axon bundle at the onset of pruning. Remarkably, decreasing axonal 
      adhesion facilitates infiltration by Arpc1 knockdown (KD) astrocytes and promotes 
      axon pruning. Conversely, increased axonal adhesion reduces lobe infiltration by 
      wild-type (WT) astrocytes. Together, our findings suggest that actin-dependent 
      astrocytic infiltration is a key step in axon pruning, thus promoting our 
      understanding of neuron-glia interactions during remodeling.
CI  - Copyright (c) 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Marmor-Kollet, Neta
AU  - Marmor-Kollet N
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 
      7610001, Israel; Department of Molecular Neuroscience, Weizmann Institute of 
      Science, Rehovot 7610001, Israel.
FAU - Berkun, Victoria
AU  - Berkun V
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 
      7610001, Israel.
FAU - Cummings, Gideon
AU  - Cummings G
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 
      7610001, Israel.
FAU - Keren-Shaul, Hadas
AU  - Keren-Shaul H
AD  - Department of Immunology, Weizmann Institute of Science, Rehovot 7610001, Israel.
FAU - David, Eyal
AU  - David E
AD  - Department of Immunology, Weizmann Institute of Science, Rehovot 7610001, Israel.
FAU - Addadi, Yoseph
AU  - Addadi Y
AD  - Weizmann Institute of Science, Life Sciences Core Facilities, Rehovot 7610001, 
      Israel.
FAU - Schuldiner, Oren
AU  - Schuldiner O
AD  - Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 
      7610001, Israel; Department of Molecular Neuroscience, Weizmann Institute of 
      Science, Rehovot 7610001, Israel. Electronic address: 
      oren.schuldiner@weizmann.ac.il.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230214
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Actins)
RN  - 0 (Drosophila Proteins)
SB  - IM
MH  - Animals
MH  - *Actins/metabolism
MH  - Astrocytes/metabolism
MH  - Axons/metabolism
MH  - Drosophila/metabolism
MH  - *Drosophila Proteins/genetics/metabolism
MH  - Neurons/metabolism
PMC - PMC9989824
OTO - NOTNLM
OT  - CP: Cell biology
OT  - CP: Neuroscience
OT  - RNA-seq
OT  - actin dynamics
OT  - astrocytes
OT  - axon adhesion
OT  - axon pruning
OT  - drosophila
OT  - glia
OT  - mushroom body
OT  - neuro-glia interactions
OT  - neuronal remodeling
COIS- Declaration of interests The authors declare that they have no conflict of 
      interest.
EDAT- 2023/02/16 06:00
MHDA- 2023/10/04 06:43
PMCR- 2023/02/14
CRDT- 2023/02/15 12:37
PHST- 2021/12/15 00:00 [received]
PHST- 2022/10/17 00:00 [revised]
PHST- 2023/01/28 00:00 [accepted]
PHST- 2023/10/04 06:43 [medline]
PHST- 2023/02/16 06:00 [pubmed]
PHST- 2023/02/15 12:37 [entrez]
PHST- 2023/02/14 00:00 [pmc-release]
AID - S2211-1247(23)00128-6 [pii]
AID - 112117 [pii]
AID - 10.1016/j.celrep.2023.112117 [doi]
PST - ppublish
SO  - Cell Rep. 2023 Feb 28;42(2):112117. doi: 10.1016/j.celrep.2023.112117. Epub 2023 
      Feb 14.