PMID- 36791122
OWN - NLM
STAT- MEDLINE
DCOM- 20230218
LR  - 20231213
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 18
IP  - 2
DP  - 2023
TI  - IL-1beta knockdown inhibits cigarette smoke extract-induced inflammation and 
      apoptosis in vascular smooth muscle cells.
PG  - e0277719
LID - 10.1371/journal.pone.0277719 [doi]
LID - e0277719
AB  - OBJECTIVE: This study was aimed to investigate the role of interleukin-1beta (IL-1beta) 
      in cigarette smoke extract (CSE)-induced apoptosis in vascular smooth muscle 
      cells and the underlying mechanism in a rat derived cell line. METHODS: Rat 
      thoracic aortic smooth muscle cells (A7r5) were divided into six groups including 
      control, CSE (model), CSE+ overexpression empty vector (OvExp-EV), CSE+IL-1beta 
      knockdown (KD), and CSE+ IL-1beta knockdown empty vector (KD-EV). The mRNA 
      expression levels of IL-1beta and pregnancy-associated plasma protein A (PAPP-A) 
      were detected by quantitative polymerase chain reaction (qPCR). The apoptosis of 
      A7r5 cells was detected by flow cytometry. The expression levels of inflammatory 
      mediators (TNFalpha, IL-6 and IL-8) and apoptotic proteins (Bax and Bcl-2) were 
      determined by western blot. RESULTS: CSE induced significant apoptosis in 
      vascular smooth muscle cells (P < 0.01) and elevated the mRNA levels of IL-1beta and 
      PAPP-A (P < 0.01). CSE administration increased protein expression of Bax, TNF-alpha, 
      IL-6, and IL-8, with significantly reduced Bcl-2 expression (P < 0.01). IL-1beta 
      knockdown significantly decreased cell apoptosis via regulating the expression of 
      these proteins (P < 0.05 or P < 0.01). CONCLUSION: IL-1beta is involved in 
      CSE-induced PAPP-A expression and apoptosis in vascular smooth muscle cells, 
      which might be considered as a target for preventing of cardiovascular diseases 
      caused by cigarette smoking.
CI  - Copyright: (c) 2023 Jiang et al. This is an open access article distributed under 
      the terms of the Creative Commons Attribution License, which permits unrestricted 
      use, distribution, and reproduction in any medium, provided the original author 
      and source are credited.
FAU - Jiang, Hongfeng
AU  - Jiang H
AUID- ORCID: 0000-0002-9261-0433
AD  - Department of Geriatrics, Wuhan Fourth Hospital, Affiliated Puai Hospital of 
      Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 
      China.
FAU - Guo, Zhangqiang
AU  - Guo Z
AD  - Department of Emergency Medicine, Wuhan Fourth Hospital, Affiliated Puai Hospital 
      of Tongji Medical College of Huazhong University of Science and Technology, 
      Wuhan, China.
FAU - Zeng, Kun
AU  - Zeng K
AD  - Department of Geriatrics, Wuhan Fourth Hospital, Affiliated Puai Hospital of 
      Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 
      China.
FAU - Tang, Haiyan
AU  - Tang H
AD  - Department of Geriatrics, Wuhan Fourth Hospital, Affiliated Puai Hospital of 
      Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 
      China.
FAU - Tan, Hanxuan
AU  - Tan H
AD  - Department of Geriatrics, Wuhan Fourth Hospital, Affiliated Puai Hospital of 
      Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 
      China.
FAU - Min, Rui
AU  - Min R
AD  - Department of Geriatrics, Wuhan Fourth Hospital, Affiliated Puai Hospital of 
      Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 
      China.
FAU - Huang, Caihua
AU  - Huang C
AD  - Department of Geriatrics, Wuhan Fourth Hospital, Affiliated Puai Hospital of 
      Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230215
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - EC 3.4.24.- (Pregnancy-Associated Plasma Protein-A)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (IL1B protein, rat)
SB  - IM
MH  - Animals
MH  - Rats
MH  - Apoptosis
MH  - bcl-2-Associated X Protein/metabolism
MH  - *Cigarette Smoking
MH  - Inflammation/metabolism
MH  - Interleukin-1beta/genetics/metabolism
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - *Muscle, Smooth, Vascular/metabolism
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Pregnancy-Associated Plasma Protein-A/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - RNA, Messenger/metabolism
MH  - Nicotiana/genetics
PMC - PMC9931126
COIS- The authors have declared that no competing interests exist.
EDAT- 2023/02/16 06:00
MHDA- 2023/02/18 06:00
PMCR- 2023/02/15
CRDT- 2023/02/15 13:44
PHST- 2022/10/30 00:00 [received]
PHST- 2023/01/31 00:00 [accepted]
PHST- 2023/02/15 13:44 [entrez]
PHST- 2023/02/16 06:00 [pubmed]
PHST- 2023/02/18 06:00 [medline]
PHST- 2023/02/15 00:00 [pmc-release]
AID - PONE-D-22-29892 [pii]
AID - 10.1371/journal.pone.0277719 [doi]
PST - epublish
SO  - PLoS One. 2023 Feb 15;18(2):e0277719. doi: 10.1371/journal.pone.0277719. 
      eCollection 2023.