PMID- 36792675
OWN - NLM
STAT- MEDLINE
DCOM- 20230217
LR  - 20230329
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Feb 15
TI  - A distinction between Fritillaria Cirrhosa Bulbus and Fritillaria Pallidiflora 
      Bulbus via LC-MS/MS in conjunction with principal component analysis and 
      hierarchical cluster analysis.
PG  - 2735
LID - 10.1038/s41598-023-29631-8 [doi]
LID - 2735
AB  - Fritillaria Cirrhosa Bulbus (known as chuanbeimu in Chinese, FCB) is one of the 
      most used Chinese medicines for lung disease. However, a variety of substitutes 
      have entered the market, with Fritillaria Pallidiflora Bulbus (FPB) being the 
      most common. Due to their similarity in appearance, morphology, and chemical 
      composition but a large price difference, the FCB has frequently been adulterated 
      with the FPB, posing a serious challenge to the distinction and quality of the 
      FCB. Therefore, we aimed to distinguish FCB and FPB based on their main nine 
      isosteroidal alkaloid contents and test the potential of chemometrics as a 
      discrimination approach for evaluating quality. The nine major isosteroidal 
      alkaloids were measured using a liquid chromatography with tandem mass 
      spectrometry (LC-MS/MS) approach in 41 batches of FCB and 17 batches of FPB. 
      Additionally, they were categorized and distinguished using the methods of 
      hierarchical cluster analysis (HCA) and principal component analysis (PCA). 
      Quantitative analysis revealed that the nine alkaloids were present in different 
      amounts in the two types of Fritillariae bulbus. In FCB, the highest amount was 
      peimisine (17.92-123.53 mug/g) and the lowest was delavine (0.42-29.18 mug/g), 
      while in FPB, imperialine was higher (78.05-344.09 mug/g), but verticinone and 
      verticine were less than the other seven alkaloids. The FCB and FPB were 
      successfully classified and distinguished by the HCA and PCA. Taken together, the 
      method has a good linear relationship (R(2) > 0.9975). The LOD and LOQ of the 
      nine alkaloids were in the range of 0.0651-0.6510 and 0.1953-1.9531 ng/mL, 
      respectively. The intra- and inter-day precision were shown to be excellent, with 
      relative standard deviations (RSDs) below 1.63% and 2.39%, respectively. The 
      LC-MS/MS method in conjunction with HCA and PCA can effectively differentiate FCB 
      and FPB. It may be a promising strategy for quality evaluation and control at the 
      FCB.
CI  - (c) 2023. The Author(s).
FAU - Liu, Chuanlan
AU  - Liu C
AD  - Medical College of Tibet University, Lasa, 850002, People's Republic of China.
AD  - Key Laboratory of Drug-Targeting and Drug Delivery System of the Education 
      Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug, and Sichuan 
      Research Center for Drug Precision Industrial Technology, West China School of 
      Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China.
FAU - Liu, Simei
AU  - Liu S
AD  - Key Laboratory of Drug-Targeting and Drug Delivery System of the Education 
      Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug, and Sichuan 
      Research Center for Drug Precision Industrial Technology, West China School of 
      Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China.
FAU - Tse, Wai Ming
AU  - Tse WM
AD  - Nin Jiom Medicine Manufactory (H.K.) Limited, 16/F. Block A, Texaco Road, Tsuen 
      Wan, N.T., Hong Kong, People's Republic of China.
FAU - Tse, Kathy Wai Gaun
AU  - Tse KWG
AD  - Nin Jiom Medicine Manufactory (H.K.) Limited, 16/F. Block A, Texaco Road, Tsuen 
      Wan, N.T., Hong Kong, People's Republic of China.
FAU - Erbu, Aga
AU  - Erbu A
AD  - Medical College of Tibet University, Lasa, 850002, People's Republic of China.
AD  - Key Laboratory of Drug-Targeting and Drug Delivery System of the Education 
      Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug, and Sichuan 
      Research Center for Drug Precision Industrial Technology, West China School of 
      Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China.
FAU - Xiong, Hai
AU  - Xiong H
AD  - Medical College of Tibet University, Lasa, 850002, People's Republic of China.
FAU - Lanzi, Gongga
AU  - Lanzi G
AD  - Medical College of Tibet University, Lasa, 850002, People's Republic of China.
FAU - Liu, Yanyong
AU  - Liu Y
AD  - Medical College of Tibet University, Lasa, 850002, People's Republic of China.
FAU - Ye, Bengui
AU  - Ye B
AD  - Medical College of Tibet University, Lasa, 850002, People's Republic of China. 
      benguiye513@163.com.
AD  - Key Laboratory of Drug-Targeting and Drug Delivery System of the Education 
      Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug, and Sichuan 
      Research Center for Drug Precision Industrial Technology, West China School of 
      Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China. 
      benguiye513@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230215
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Alkaloids)
SB  - IM
MH  - *Fritillaria/chemistry
MH  - Chromatography, Liquid
MH  - Tandem Mass Spectrometry
MH  - Principal Component Analysis
MH  - *Alkaloids/chemistry
MH  - Cluster Analysis
PMC - PMC9931167
COIS- The authors declare no competing interests.
EDAT- 2023/02/16 06:00
MHDA- 2023/02/18 06:00
PMCR- 2023/02/15
CRDT- 2023/02/15 23:19
PHST- 2022/09/16 00:00 [received]
PHST- 2023/02/08 00:00 [accepted]
PHST- 2023/02/15 23:19 [entrez]
PHST- 2023/02/16 06:00 [pubmed]
PHST- 2023/02/18 06:00 [medline]
PHST- 2023/02/15 00:00 [pmc-release]
AID - 10.1038/s41598-023-29631-8 [pii]
AID - 29631 [pii]
AID - 10.1038/s41598-023-29631-8 [doi]
PST - epublish
SO  - Sci Rep. 2023 Feb 15;13(1):2735. doi: 10.1038/s41598-023-29631-8.