PMID- 36792923
OWN - NLM
STAT- MEDLINE
DCOM- 20230418
LR  - 20230522
IS  - 1937-3392 (Electronic)
IS  - 1937-3384 (Linking)
VI  - 29
IP  - 4
DP  - 2023 Apr
TI  - Differentiation of Human Induced Pluripotent Stem Cells into Mature and 
      Myelinating Schwann Cells.
PG  - 134-143
LID - 10.1089/ten.TEC.2022.0186 [doi]
AB  - In the peripheral nervous system, Schwann cells (SCs) play a crucial role in 
      axonal growth, metabolic support of neurons, and the production of myelin 
      sheaths. Expansion of SCs after extraction from human or animal nerves is a long 
      and often low-yielding process. We established a rapid cell culture method using 
      a defined serum-free medium to differentiate human induced pluripotent stem cells 
      (iPSCs) into SCs in only 21 days. The SC identity was characterized by expression 
      of SRY-Box Transcription factor 10 (SOX10), S100b, glial fibrillary acidic 
      protein (GFAP), P75, growth-associated protein 43 (GAP43), and early growth 
      response 2 (EGR2) markers. The SC purity reached 87% as assessed by flow 
      cytometry using the specific SOX10 marker, and 69% based on S100b expression. 
      When SCs were cocultured with iPSC-derived motor neurons two-dimensionally or 
      three-dimensionally (3D), they also expressed the markers of myelin MBP, MPZ, and 
      gliomedin. Likewise, when they were seeded on the opposite side of a porous 
      collagen sponge from motor neurons in the 3D model, they were able to migrate 
      through it and colocalize with motor axons after 8 weeks of maturation. Moreover, 
      they were shown by transmission electron microscopy to form myelin sheaths around 
      motor axons. These results suggest that the use of autologous iPSC-derived SCs 
      for clinical applications such as the repair of peripheral nerve damage, the 
      treatment of spinal cord injuries, or for demyelinating diseases could be a 
      valuable option. Impact Statement Peripheral nerve injuries can cause the 
      complete paralysis of the upper or lower limbs, which considerably reduces the 
      quality of life of patients. To repair this injury, many approaches have been 
      developed by tissue engineering. Combining biomaterials with Schwann cells (SCs) 
      has been shown to be an effective solution for stimulating nerve regeneration. 
      However, the challenge faced concerns the strategy for obtaining autologous SCs 
      to treat patients. A promising approach is to differentiate them from the 
      patient's own cells, previously induced into pluripotent stem cells. We propose a 
      fast culture method to generate functional SCs differentiated from induced 
      pluripotent stem cells.
FAU - Louit, Aurelie
AU  - Louit A
AD  - LOEX, Centre de recherche du CHU de Quebec-Universite Laval, Quebec City, Quebec, 
      Canada.
FAU - Beaudet, Marie-Josee
AU  - Beaudet MJ
AD  - LOEX, Centre de recherche du CHU de Quebec-Universite Laval, Quebec City, Quebec, 
      Canada.
FAU - Pepin, Remy
AU  - Pepin R
AD  - LOEX, Centre de recherche du CHU de Quebec-Universite Laval, Quebec City, Quebec, 
      Canada.
FAU - Berthod, Francois
AU  - Berthod F
AD  - LOEX, Centre de recherche du CHU de Quebec-Universite Laval, Quebec City, Quebec, 
      Canada.
AD  - Department of Surgery, Faculty of Medicine, Universite Laval, Quebec City, 
      Quebec, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230316
PL  - United States
TA  - Tissue Eng Part C Methods
JT  - Tissue engineering. Part C, Methods
JID - 101466663
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Induced Pluripotent Stem Cells
MH  - Quality of Life
MH  - Schwann Cells
MH  - Myelin Sheath/metabolism
MH  - Cell Differentiation
MH  - Nerve Regeneration/physiology
MH  - Cells, Cultured
OTO - NOTNLM
OT  - Glial cells
OT  - Schwann cells
OT  - differentiation protocol
OT  - iPSC
OT  - myelin
OT  - neuron
OT  - tissue engineering
EDAT- 2023/02/16 06:00
MHDA- 2023/04/18 06:42
CRDT- 2023/02/15 23:33
PHST- 2023/04/18 06:42 [medline]
PHST- 2023/02/16 06:00 [pubmed]
PHST- 2023/02/15 23:33 [entrez]
AID - 10.1089/ten.TEC.2022.0186 [doi]
PST - ppublish
SO  - Tissue Eng Part C Methods. 2023 Apr;29(4):134-143. doi: 
      10.1089/ten.TEC.2022.0186. Epub 2023 Mar 16.