PMID- 36796802
OWN - NLM
STAT- MEDLINE
DCOM- 20230404
LR  - 20230407
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 57
IP  - 7
DP  - 2023 Apr
TI  - The effects of delta-9-tetrahydrocannabinol administration in the regulation of 
      female rat sociosexual behaviour.
PG  - 1068-1080
LID - 10.1111/ejn.15939 [doi]
AB  - By targeting the endocannabinoid system, delta-9-tetrahydrocannabinol (THC) 
      modulates female motivated behaviours, influenced by sex hormones. Both medial 
      preoptic nucleus (MPN) and ventromedial nucleus of the hypothalamus (VMN) are 
      involved in the modulation of female sexual responses. The first triggers 
      proceptivity, whereas the ventrolateral division of the latter (VMNvl) triggers 
      receptivity. These nuclei are modulated by glutamate, which inhibits female 
      receptivity, and GABA, which has a dichotomous action in female sexual 
      motivation. Here, we evaluated the action of THC on the modulation of social and 
      sexual behaviours, on signalling pathways of MPN and VMNvl and how sex hormones 
      influence these parameters. Young ovariectomized female rats, given sex hormones 
      (oestradiol benzoate, EB, and progesterone, P) and THC were used for behavioural 
      testing and for immunofluorescence analyses of vesicular glutamate transporter 2 
      (VGlut2) and GAD (glutamic acid decarboxylase)67 expression. Results showed that 
      females given EB + P exhibited a higher preference for male partner, as well as 
      higher proceptivity and a higher receptivity than control or females given only 
      EB. Females treated with THC presented similar responses in control or EB + P 
      female rats and even more facilitated behavioural responses in EB females than 
      the ones that did not receive THC. Immunofluorescence results in the MPN 
      exhibited a decreased expression of GAD67 and VGlut2 in EB + THC-treated female 
      rats. Within VMNvl of EB-primed rats no changes in the expression of both 
      proteins were observed after THC exposure. This study demonstrates how the 
      possible outcomes of endocannabinoid system instability within hypothalamic 
      neuron connectivity can modify female rat sociosexual behaviour.
CI  - (c) 2023 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
FAU - Costa, Flavia M
AU  - Costa FM
AUID- ORCID: 0000-0002-0793-5904
AD  - Department of Biomedicine, Unit of Anatomy, Faculty of Medicine, University of 
      Porto, Porto, Portugal.
FAU - Fonseca, Bruno M
AU  - Fonseca BM
AUID- ORCID: 0000-0002-8873-5591
AD  - UCIBIO - Applied Molecular Biosciences Unit, Laboratory of Biochemistry, 
      Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 
      Porto, Portugal.
AD  - Associate Laboratory i4HB - Institute for Health and Bioeconomy, Faculty of 
      Pharmacy, University of Porto, Porto, Portugal.
FAU - Sa, Susana I
AU  - Sa SI
AUID- ORCID: 0000-0002-5078-980X
AD  - Department of Biomedicine, Unit of Anatomy, Faculty of Medicine, University of 
      Porto, Porto, Portugal.
AD  - CINTESIS@RISE, Department of Biomedicine, Unit of Anatomy, Faculty of Medicine, 
      University of Porto, Porto, Portugal.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230228
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 7J8897W37S (Dronabinol)
RN  - 0 (Endocannabinoids)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Female
MH  - Male
MH  - Humans
MH  - *Dronabinol/pharmacology
MH  - *Sexual Behavior, Animal/physiology
MH  - Endocannabinoids
MH  - Progesterone
MH  - Estradiol/pharmacology/physiology
MH  - Hypothalamus
MH  - Ovariectomy
OTO - NOTNLM
OT  - cannabinoids
OT  - hypothalamus
OT  - neurotransmission
OT  - oestrogen
OT  - progesterone
EDAT- 2023/02/17 06:00
MHDA- 2023/04/04 06:42
CRDT- 2023/02/16 20:12
PHST- 2023/01/31 00:00 [revised]
PHST- 2022/04/26 00:00 [received]
PHST- 2023/02/12 00:00 [accepted]
PHST- 2023/04/04 06:42 [medline]
PHST- 2023/02/17 06:00 [pubmed]
PHST- 2023/02/16 20:12 [entrez]
AID - 10.1111/ejn.15939 [doi]
PST - ppublish
SO  - Eur J Neurosci. 2023 Apr;57(7):1068-1080. doi: 10.1111/ejn.15939. Epub 2023 Feb 
      28.