PMID- 36797305
OWN - NLM
STAT- MEDLINE
DCOM- 20230222
LR  - 20230419
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Feb 16
TI  - Metagenomic mapping of cyanobacteria and potential cyanotoxin producing taxa in 
      large rivers of the United States.
PG  - 2806
LID - 10.1038/s41598-023-29037-6 [doi]
LID - 2806
AB  - Cyanobacteria and cyanotoxin producing cyanobacterial blooms are a trending focus 
      of current research. Many studies focus on bloom events in lentic environments 
      such as lakes or ponds. Comparatively few studies have explored lotic 
      environments and fewer still have examined the cyanobacterial communities and 
      potential cyanotoxin producers during ambient, non-bloom conditions. Here we used 
      a metagenomics-based approach to profile non-bloom microbial communities and 
      cyanobacteria in 12 major U.S. rivers at multiple time points during the summer 
      months of 2019. Our data show that U.S. rivers possess microbial communities that 
      are taxonomically rich, yet largely consistent across geographic location and 
      time. Within these communities, cyanobacteria often comprise significant portions 
      and frequently include multiple species with known cyanotoxin producing strains. 
      We further characterized these potential cyanotoxin producing taxa by deep 
      sequencing amplicons of the microcystin E (mcyE) gene. We found that rivers 
      containing the highest levels of potential cyanotoxin producing cyanobacteria 
      consistently possess taxa with the genetic potential for cyanotoxin production 
      and that, among these taxa, the predominant genus of origin for the mcyE gene is 
      Microcystis. Combined, these data provide a unique perspective on cyanobacteria 
      and potential cyanotoxin producing taxa that exist in large rivers across the 
      U.S. and can be used to better understand the ambient conditions that may precede 
      bloom events in lotic freshwater ecosystems.
CI  - (c) 2023. This is a U.S. Government work and not under copyright protection in the 
      US; foreign copyright protection may apply.
FAU - Linz, David M
AU  - Linz DM
AD  - Office of Research and Development, U.S. Environmental Protection Agency, 
      Cincinnati, OH, USA.
FAU - Sienkiewicz, Nathan
AU  - Sienkiewicz N
AD  - Office of Research and Development, U.S. Environmental Protection Agency, 
      Cincinnati, OH, USA.
FAU - Struewing, Ian
AU  - Struewing I
AD  - Office of Research and Development, U.S. Environmental Protection Agency, 
      Cincinnati, OH, USA.
FAU - Stelzer, Erin A
AU  - Stelzer EA
AD  - U.S. Geological Survey, Columbus, OH, USA.
FAU - Graham, Jennifer L
AU  - Graham JL
AD  - U.S. Geological Survey, Troy, NY, USA.
FAU - Lu, Jingrang
AU  - Lu J
AD  - Office of Research and Development, U.S. Environmental Protection Agency, 
      Cincinnati, OH, USA. lu.jingrang@epa.gov.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20230216
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Microcystins)
SB  - IM
MH  - United States
MH  - *Cyanobacteria/genetics
MH  - *Microcystis
MH  - Rivers/microbiology
MH  - Lakes/microbiology
MH  - Microcystins/genetics
MH  - *Microbiota
PMC - PMC9935515
COIS- The authors declare no competing interests.
EDAT- 2023/02/17 06:00
MHDA- 2023/02/22 06:00
PMCR- 2023/02/16
CRDT- 2023/02/16 23:17
PHST- 2022/06/02 00:00 [received]
PHST- 2023/01/30 00:00 [accepted]
PHST- 2023/02/16 23:17 [entrez]
PHST- 2023/02/17 06:00 [pubmed]
PHST- 2023/02/22 06:00 [medline]
PHST- 2023/02/16 00:00 [pmc-release]
AID - 10.1038/s41598-023-29037-6 [pii]
AID - 29037 [pii]
AID - 10.1038/s41598-023-29037-6 [doi]
PST - epublish
SO  - Sci Rep. 2023 Feb 16;13(1):2806. doi: 10.1038/s41598-023-29037-6.