PMID- 36798808
OWN - NLM
STAT- MEDLINE
DCOM- 20230222
LR  - 20230222
IS  - 1177-8881 (Electronic)
IS  - 1177-8881 (Linking)
VI  - 17
DP  - 2023
TI  - Preparation of Cod Skin Collagen Peptides/Chitosan-Based Temperature-Sensitive 
      Gel and Its Anti-Photoaging Effect in Skin.
PG  - 419-437
LID - 10.2147/DDDT.S391812 [doi]
AB  - BACKGROUND: Photoaging decreases quality of life and increases the risk of skin 
      cancer, underscoring the urgent need to explore natural, high-efficacy, anti-skin 
      photoaging (SP) active substances. METHODS: In this study, a gel (CS/CSCPs/beta-GP 
      gel) was prepared using chitosan (CS) and sodium beta-glycerophosphate (beta-GP) 
      through crosslinking with small molecular CSCPs as the carried drug. We evaluated 
      its structural characteristics and properties. The effect of CS/CSCPs/beta-GP gel on 
      the degree of ultraviolet (UV)-induced skin aging of mice was investigated 
      through comparative analysis of skin damage, the integrity of collagen tissues 
      and elastic fibers, levels of reactive oxygen species (ROS) and key inflammatory 
      factors (tumor necrosis factor [TNF]-alpha and interleukin [IL]-1beta, IL-6, and IL-10), 
      and tissue expression of matrix metalloproteinase-3 (MMP-3) after repeated UV 
      irradiation in a nude mice SP model. RESULTS: The results showed that 
      CS/CSCPs/beta-GP gel was successfully prepared and had the desired characteristics. 
      Compared with CSCPs alone, the CS/CSCPs/beta-GP gel more evidently improved typical 
      photoaging characteristics on mouse dorsal skin. It also increased the moisture 
      content, causing the skin to become glossy and elastic. Pathological skin 
      analysis revealed that this peptide-carrying gel can effectively inhibit 
      epidermal thickening, reduce tissue inflammatory infiltration, suppress collagen 
      fiber degradation, increase the collagen content, alleviate structural elastic 
      fiber damage, and significantly inhibit abnormal MMP-3 expression. In addition, 
      biochemical analysis showed that the CS/CSCPs/beta-GP gel can effectively inhibit 
      the elevated expressions of ROS and key proinflammatory factors (TNF-alpha, IL-1beta, 
      IL-6) in photoaging skin tissues and promote expression of the anti-inflammatory 
      factor IL-10. CONCLUSION: SP can cause many clinical skin diseases, such as solar 
      freckle-like nevus, solar keratosis, cutaneous melanoma, and squamous cell 
      carcinoma. CSCPs are a high-efficacy anti-SP natural active substance and 
      CS/CSCPs/beta-GP gel can synergistically enhance the CSCPs' anti-SP effect. The 
      mechanism is likely related to the inhibited activation of ROS/nuclear 
      transcription factor-kappaB signaling and the expression of downstream inflammatory 
      factors.
CI  - (c) 2023 Kong et al.
FAU - Kong, Songzhi
AU  - Kong S
AD  - School of Chemistry and Environmental Science, Guangdong Ocean University, 
      Zhanjiang, 524088, People's Republic of China.
FAU - Lv, Lijiao
AU  - Lv L
AD  - School of Chemistry and Environmental Science, Guangdong Ocean University, 
      Zhanjiang, 524088, People's Republic of China.
FAU - Guo, Jiaqi
AU  - Guo J
AD  - School of Chemistry and Environmental Science, Guangdong Ocean University, 
      Zhanjiang, 524088, People's Republic of China.
FAU - Yang, Xiaohong
AU  - Yang X
AD  - School of Chemistry and Environmental Science, Guangdong Ocean University, 
      Zhanjiang, 524088, People's Republic of China.
FAU - Liao, Mingneng
AU  - Liao M
AD  - School of Chemistry and Environmental Science, Guangdong Ocean University, 
      Zhanjiang, 524088, People's Republic of China.
FAU - Zhao, Tianyang
AU  - Zhao T
AD  - School of Chemistry and Environmental Science, Guangdong Ocean University, 
      Zhanjiang, 524088, People's Republic of China.
FAU - Sun, Haiyang
AU  - Sun H
AD  - School of Chemistry and Environmental Science, Guangdong Ocean University, 
      Zhanjiang, 524088, People's Republic of China.
FAU - Zhang, Shuqun
AU  - Zhang S
AD  - School of Chemistry and Environmental Science, Guangdong Ocean University, 
      Zhanjiang, 524088, People's Republic of China.
FAU - Li, Wenjie
AU  - Li W
AD  - Department of Hematology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, Guangdong, 524001, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20230210
PL  - New Zealand
TA  - Drug Des Devel Ther
JT  - Drug design, development and therapy
JID - 101475745
RN  - 130068-27-8 (Interleukin-10)
RN  - 9012-76-4 (Chitosan)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Interleukin-6)
RN  - 9007-34-5 (Collagen)
RN  - 0 (Peptides)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Interleukin-10
MH  - *Chitosan/pharmacology
MH  - Matrix Metalloproteinase 3/metabolism
MH  - *Melanoma
MH  - Reactive Oxygen Species
MH  - Mice, Nude
MH  - Interleukin-6
MH  - Quality of Life
MH  - Temperature
MH  - *Skin Neoplasms
MH  - Collagen
MH  - *Skin Diseases
MH  - *Skin Aging
MH  - Peptides/pharmacology
MH  - NF-kappa B/metabolism
MH  - Ultraviolet Rays
PMC - PMC9926988
OTO - NOTNLM
OT  - ROS/NF-kappaB
OT  - chitosan
OT  - cod skin collagen peptides
OT  - skin photoaging
OT  - temperature-sensitive gel
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/18 06:00
MHDA- 2023/02/22 06:00
PMCR- 2023/02/10
CRDT- 2023/02/17 02:16
PHST- 2022/10/05 00:00 [received]
PHST- 2023/01/21 00:00 [accepted]
PHST- 2023/02/17 02:16 [entrez]
PHST- 2023/02/18 06:00 [pubmed]
PHST- 2023/02/22 06:00 [medline]
PHST- 2023/02/10 00:00 [pmc-release]
AID - 391812 [pii]
AID - 10.2147/DDDT.S391812 [doi]
PST - epublish
SO  - Drug Des Devel Ther. 2023 Feb 10;17:419-437. doi: 10.2147/DDDT.S391812. 
      eCollection 2023.