PMID- 36799151
OWN - NLM
STAT- MEDLINE
DCOM- 20230222
LR  - 20230223
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 27
IP  - 3
DP  - 2023 Mar
TI  - Expression and significance of histone methyltransferase SET domain containing 2 
      with histone H3 lysine 36 trimethylation in mouse hepatic oval cells 
      differentiated into bile duct epithelial cells in vitro.
LID - 69 [pii]
LID - 10.3892/mmr.2023.12956 [doi]
AB  - The present study aimed to identify the function and expression of trimethylated 
      protein histone H3 lysine 36 (H3K36)me3 and the upstream specific enzyme histone 
      methyltransferase SET domain containing 2 (SETD2), during the differentiation of 
      hepatic oval cells (HOCs) into cholangiocytes in mice following partial liver 
      resection and fed with 2‑acetamidofluorene. HOCs were isolated from Kunming male 
      mice fed with 2‑acetamidofluorene for 10 days. Their liver tissues were then 
      isolated following partial liver resection and another week of 
      2‑acetamidofluorene treatment. HOCs were collected following a two‑step enzyme 
      digestion procedure involving protease E and collagenase 4. The target cells were 
      cultured in DMEM/F12 supplemented with 10 microg/ml EGF, 5 microg/ml stem cell growth 
      factor and 5 microg/ml leukemia inhibitory factor. Target cells using the markers 
      OV‑6, CK‑19, SETD2, H3K36me3, were detected with flow cytometry and 
      immunofluorescence microscopy; reverse transcription‑quantitative PCR and western 
      blotting were used to quantify the protein levels of SETD2 and H3K36me3. The 
      retrieved primary hepatocytes developed into cholangiocytes with increasing CK‑19 
      and decreasing OV‑6 expression in each subsequent passage, whereas the SETD2 and 
      H3K36me3 levels gradually increased, suggesting the possible involvement of both 
      of these factors in differentiation.
FAU - Jin, Liquan
AU  - Jin L
AD  - First Department of General Surgery, The First Affiliated Hospital of Dali 
      University, P.R. China.
FAU - Su, Ziting
AU  - Su Z
AD  - First Department of General Surgery, The First Affiliated Hospital of Dali 
      University, P.R. China.
FAU - Huang, Shan
AU  - Huang S
AD  - First Department of General Surgery, The First Affiliated Hospital of Dali 
      University, P.R. China.
FAU - Tan, Yunbo
AU  - Tan Y
AD  - First Department of General Surgery, The First Affiliated Hospital of Dali 
      University, P.R. China.
FAU - Mrema, Isack George
AU  - Mrema IG
AD  - Clinical Medical College, Dali University, Dali, Yunnan 671000, P.R. China.
FAU - Chen, Yiming
AU  - Chen Y
AD  - First Department of General Surgery, The First Affiliated Hospital of Dali 
      University, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20230217
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Histones)
RN  - EC 2.1.1.- (Histone Methyltransferases)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Mice
MH  - Male
MH  - Animals
MH  - *Histones/metabolism
MH  - Histone Methyltransferases/metabolism
MH  - *Lysine/metabolism
MH  - PR-SET Domains
MH  - Cell Differentiation
MH  - Epithelial Cells/metabolism
MH  - Bile Ducts/metabolism
PMC - PMC9942252
OTO - NOTNLM
OT  - bile duct epithelial cells
OT  - differentiation
OT  - histone H3 lysine 36 trimethylation
OT  - histone methyltransferase SET domain containing 2
OT  - oval cell
COIS- The authors declare that they have no competing interests.
EDAT- 2023/02/18 06:00
MHDA- 2023/02/22 06:00
PMCR- 2023/02/08
CRDT- 2023/02/17 04:32
PHST- 2022/05/24 00:00 [received]
PHST- 2022/12/30 00:00 [accepted]
PHST- 2023/02/17 04:32 [entrez]
PHST- 2023/02/18 06:00 [pubmed]
PHST- 2023/02/22 06:00 [medline]
PHST- 2023/02/08 00:00 [pmc-release]
AID - 69 [pii]
AID - MMR-27-3-12956 [pii]
AID - 10.3892/mmr.2023.12956 [doi]
PST - ppublish
SO  - Mol Med Rep. 2023 Mar;27(3):69. doi: 10.3892/mmr.2023.12956. Epub 2023 Feb 17.