PMID- 36799910
OWN - NLM
STAT- Publisher
LR  - 20240216
IS  - 1744-5078 (Electronic)
IS  - 0927-3948 (Linking)
DP  - 2023 Feb 17
TI  - In Vitro Expression Analysis of Cytokines and ROS-Related Genes in Human Corneal 
      Fibroblasts and Keratocytes of Healthy and Keratoconus Corneas.
PG  - 1-10
LID - 10.1080/09273948.2023.2176325 [doi]
AB  - PURPOSE: To investigate expression of cytokines and ROS-related genes in stromal 
      cells of healthy and keratoconus (KC) corneas. METHODS: Expression analysis was 
      performed for cytokines including several interleukins (IL), Tumor necrosis 
      factor-alpha (TNF-alpha), Transforming growth factor-beta1 (TGF-beta1), Interferon-gamma (IFN-gamma) 
      and ROS-related genes such as Catalase, Glutathione peroxidase 1, NADPH oxidase 
      1, superoxide dismutase 1 in corneal fibroblasts (HCFs/KC-HCFs) or keratocytes 
      (Keratocytes/KC-Keratocytes) by qPCR and ELISA. RESULTS: Gene and protein 
      expression of most inflammatory markers was decreased in keratocytes compared to 
      fibroblasts, whereas no differences were found between healthy and keratoconus 
      cells for the majority of cytokines measured. TNF-alpha expression was increased at 
      gene (KC keratocytes) and protein levels (supernatant of 
      Keratocytes/KC-Keratocytes) compared to corneal fibroblasts. No differential 
      expression of ROS-related genes was detected between healthy and diseased cells 
      in both fibroblasts and keratocytes. CONCLUSION: Increased expression of several 
      inflammatory markers described as altered in KC was not evident in KC cells in 
      vitro.
FAU - Berger, Tim
AU  - Berger T
AUID- ORCID: 0000-0003-2307-2237
AD  - Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, 
      Germany.
FAU - Szentmary, Nora
AU  - Szentmary N
AUID- ORCID: 0000-0001-8019-1481
AD  - Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Congenital Aniridia 
      Research, Saarland University, Homburg/Saar, Germany.
FAU - Chai, Ning
AU  - Chai N
AD  - Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Congenital Aniridia 
      Research, Saarland University, Homburg/Saar, Germany.
FAU - Flockerzi, Elias
AU  - Flockerzi E
AD  - Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, 
      Germany.
FAU - Daas, Loay
AU  - Daas L
AD  - Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, 
      Germany.
FAU - Stachon, Tanja
AU  - Stachon T
AD  - Dr. Rolf M. Schwiete Center for Limbal Stem Cell and Congenital Aniridia 
      Research, Saarland University, Homburg/Saar, Germany.
FAU - Seitz, Berthold
AU  - Seitz B
AUID- ORCID: 0000-0001-9701-8204
AD  - Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20230217
PL  - England
TA  - Ocul Immunol Inflamm
JT  - Ocular immunology and inflammation
JID - 9312169
SB  - IM
OTO - NOTNLM
OT  - Corneal fibroblasts
OT  - cytokines
OT  - inflammation
OT  - keratoconus
OT  - keratocytes
OT  - reactive oxygen species
EDAT- 2023/02/18 06:00
MHDA- 2023/02/18 06:00
CRDT- 2023/02/17 10:53
PHST- 2023/02/17 10:53 [entrez]
PHST- 2023/02/18 06:00 [pubmed]
PHST- 2023/02/18 06:00 [medline]
AID - 10.1080/09273948.2023.2176325 [doi]
PST - aheadofprint
SO  - Ocul Immunol Inflamm. 2023 Feb 17:1-10. doi: 10.1080/09273948.2023.2176325.