PMID- 36802594
OWN - NLM
STAT- MEDLINE
DCOM- 20230307
LR  - 20230307
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 71
IP  - 8
DP  - 2023 Mar 1
TI  - Walnut-Derived Peptides Promote Autophagy via the Activation of AMPK/mTOR/ULK1 
      Pathway to Ameliorate Hyperglycemia in Type 2 Diabetic Mice.
PG  - 3751-3765
LID - 10.1021/acs.jafc.2c07112 [doi]
AB  - Autophagy flux plays a significant protective role in type 2 diabetes mellitus 
      (T2DM). However, the mechanisms by which autophagy mediates insulin resistance 
      (IR) to ameliorate T2DM remain unclear. This study explored the hypoglycemic 
      effects and mechanisms of walnut-derived peptides (fraction 3-10 kDa and LP5) in 
      streptozotocin and high-fat-diet-induced T2DM mice. Findings revealed that 
      walnut-derived peptides reduced the levels of blood glucose and FINS and 
      ameliorated IR and dyslipidemia. They also increased SOD and GSH-P(X) activities 
      and inhibited the secretion of TNF-alpha, IL-6, and IL-1beta. Additionally, they 
      increased the levels of ATP, COX, SDH, and MMP of liver mitochondria. Western 
      blotting indicated that walnut-derived peptides up-regulated LC3-II/LC3-I and 
      Beclin-1 expression, while they down-regulated p62 expression, which may be 
      associated with the activation of the AMPK/mTOR/ULK1 pathway. Finally, the AMPK 
      activator (AICAR) and inhibitor (Compound C) were used to verify that LP5 could 
      activate autophagy through the AMPK/mTOR/ULK1 pathway in IR HepG2 cells.
FAU - Hou, Weiyu
AU  - Hou W
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Zhao, Fanrui
AU  - Zhao F
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Fang, Li
AU  - Fang L
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Wang, Xiyan
AU  - Wang X
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Wu, Dan
AU  - Wu D
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Liu, Chunlei
AU  - Liu C
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Leng, Yue
AU  - Leng Y
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Gao, Yawen
AU  - Gao Y
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Fu, Junxi
AU  - Fu J
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Wang, Ji
AU  - Wang J
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
FAU - Min, Weihong
AU  - Min W
AUID- ORCID: 0000-0002-6093-2104
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, People's Republic of China.
AD  - National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 
      130118, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20230219
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - 0 (Peptides)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (Plant Proteins)
RN  - EC 2.7.11.1 (Ulk1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - AMP-Activated Protein Kinases/genetics/metabolism
MH  - Autophagy
MH  - *Diabetes Mellitus, Experimental/drug therapy
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - *Hyperglycemia/drug therapy
MH  - *Juglans/metabolism
MH  - Peptides/pharmacology
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Plant Proteins/pharmacology
MH  - Signal Transduction
OTO - NOTNLM
OT  - AMPK/mTOR/ULK1
OT  - autophagy
OT  - insulin resistance
OT  - type 2 diabetes mellitus
OT  - walnut-derived peptides
EDAT- 2023/02/22 06:00
MHDA- 2023/03/03 06:00
CRDT- 2023/02/21 17:21
PHST- 2023/02/22 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/21 17:21 [entrez]
AID - 10.1021/acs.jafc.2c07112 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2023 Mar 1;71(8):3751-3765. doi: 10.1021/acs.jafc.2c07112. 
      Epub 2023 Feb 19.