PMID- 36806924
OWN - NLM
STAT- MEDLINE
DCOM- 20230307
LR  - 20230307
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 200
DP  - 2023 Apr 1
TI  - Extremely low-frequency electromagnetic field (ELF-EMF) induces alterations in 
      epigenetic regulation in the myometrium - An in vitro study.
PG  - 136-146
LID - S0093-691X(23)00042-0 [pii]
LID - 10.1016/j.theriogenology.2023.02.005 [doi]
AB  - Previous research by the authors indicated that an extremely low-frequency 
      electromagnetic field (ELF-EMF) evokes molecular alterations in the porcine 
      myometrium. It was hypothesized that the ELF-EMF could induce alterations in the 
      epigenetic regulation of gene expression in the myometrium. In the current study, 
      slices of the porcine myometrium during the peri-implantation period (n = 4) were 
      used for further in vitro exposition to ELF-EMF (50 Hz, 8 mT, 2 h treatment 
      duration). The study tested whether the ELF-EMF may affect: 1/the expression of 
      DNA (cytosine-5)-methyltransferase 1 (DNMT1) and DNA 
      (cytosine-5)-methyltransferase 3a (DNMT3a), 2/the level of genomic DNA 
      methylation, and 3/the level of amplification of methylated and unmethylated 
      variants of promoter regions of selected genes with altered expression in 
      response to ELF-EMF. It was found that ELF-EMF treatment increased DNMT1, 
      decreased DNMT3a mRNA transcript and protein abundance, and increased the level 
      of genomic DNA methylation. The direction of alterations in the level of 
      amplification of methylated and unmethylated variants of the promoter region of 
      selected genes with altered expression, i.e. prodynorphin (PDYN), interleukin 15 
      (IL15) signal transducer and activator of transcription 5A (STAT5A), tumor 
      necrosis factor (TNF), and between down-regulated genes were early growth 
      response 2 (EGR2), hyaluronan and proteoglycan link protein 1 (HAPLN1), and 
      uteroferrin associated basic protein-2 (UABP2), mostly involving the direction of 
      changes in their transcriptional activity, which was evaluated in a previous 
      study by the authors. Thus, ELF-EMF radiation disturbs epigenetic mechanisms, 
      which may underlay ELF-EMF-related transcriptomic alterations in the myometrium.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Franczak, Anita
AU  - Franczak A
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10- 
      719, Olsztyn, Poland. Electronic address: anitaf@uwm.edu.pl.
FAU - Drzewiecka, Ewa Monika
AU  - Drzewiecka EM
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10- 
      719, Olsztyn, Poland. Electronic address: e.drzewiecka@pan.olsztyn.pl.
FAU - Kozlowska, Wiktoria
AU  - Kozlowska W
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10- 
      719, Olsztyn, Poland.
FAU - Zmijewska, Agata
AU  - Zmijewska A
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10- 
      719, Olsztyn, Poland.
FAU - Wydorski, Pawel Jozef
AU  - Wydorski PJ
AD  - Department of Animal Anatomy and Physiology, Faculty of Biology and 
      Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10- 
      719, Olsztyn, Poland.
LA  - eng
PT  - Journal Article
DEP - 20230207
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Female
MH  - Animals
MH  - Swine
MH  - *Myometrium
MH  - *Electromagnetic Fields
MH  - Epigenesis, Genetic
MH  - DNA
MH  - Gene Expression Profiling/veterinary
OTO - NOTNLM
OT  - DNA methylation
OT  - Electromagnetic field
OT  - Epigenetic regulation
OT  - Methyltransferases
OT  - Myometrium
EDAT- 2023/02/23 06:00
MHDA- 2023/03/08 06:00
CRDT- 2023/02/22 11:07
PHST- 2022/11/03 00:00 [received]
PHST- 2023/01/16 00:00 [revised]
PHST- 2023/02/04 00:00 [accepted]
PHST- 2023/02/23 06:00 [pubmed]
PHST- 2023/03/08 06:00 [medline]
PHST- 2023/02/22 11:07 [entrez]
AID - S0093-691X(23)00042-0 [pii]
AID - 10.1016/j.theriogenology.2023.02.005 [doi]
PST - ppublish
SO  - Theriogenology. 2023 Apr 1;200:136-146. doi: 
      10.1016/j.theriogenology.2023.02.005. Epub 2023 Feb 7.