PMID- 36807786
OWN - NLM
STAT- MEDLINE
DCOM- 20230224
LR  - 20230305
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2615
DP  - 2023
TI  - Visualization of mtDNA Using FISH.
PG  - 89-98
LID - 10.1007/978-1-0716-2922-2_7 [doi]
AB  - Proper mitochondrial DNA (mtDNA) levels are critical for many cellular biological 
      functions and are associated with aging and many mitochondria disorders. Defects 
      in core subunits of the mtDNA replication machinery lead to decreased mtDNA 
      levels. Other indirect mitochondrial contexts including ATP concentration, lipid 
      composition, and nucleotide composition also contribute to mtDNA maintenance. 
      Furthermore, mtDNA molecules are distributed evenly throughout the mitochondrial 
      network. This uniform distribution pattern is required for oxidative 
      phosphorylation and ATP production and has been linked to many diseases when 
      perturbed. Thus, it is important to visualize mtDNA in the cellular context. Here 
      we provide detailed protocols for cellular visualization of mtDNA using 
      fluorescence in situ hybridization (FISH). The fluorescent signals are targeted 
      to the mtDNA sequence directly, ensuring both sensitivity and specificity. This 
      mtDNA FISH method can be combined with immunostaining and used for visualizing 
      mtDNA-protein interactions and dynamics.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Xie, Xie
AU  - Xie X
AD  - Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 
      Gothenburg, Sweden.
FAU - Zhu, Xuefeng
AU  - Zhu X
AD  - Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 
      Gothenburg, Sweden. xuefeng.zhu@gu.se.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Mitochondrial)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - *DNA, Mitochondrial/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Mitochondria/genetics
MH  - DNA Replication
MH  - Adenosine Triphosphate
OTO - NOTNLM
OT  - FISH
OT  - Imaging
OT  - Microscopy
OT  - Mitochondria
OT  - Visualization
OT  - mtDNA
EDAT- 2023/02/23 06:00
MHDA- 2023/02/25 06:00
CRDT- 2023/02/22 12:11
PHST- 2023/02/22 12:11 [entrez]
PHST- 2023/02/23 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
AID - 10.1007/978-1-0716-2922-2_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2615:89-98. doi: 10.1007/978-1-0716-2922-2_7.