PMID- 36807912
OWN - NLM
STAT- MEDLINE
DCOM- 20230606
LR  - 20230606
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 103
IP  - 9
DP  - 2023 Jul
TI  - Digestion profile, antioxidant, and antidiabetic capacity of Morchella esculenta 
      exopolysaccharide: in vitro, in vivo and microbiota analysis.
PG  - 4401-4412
LID - 10.1002/jsfa.12513 [doi]
AB  - BACKGROUND: Novel functional polysaccharides from fungi are important 
      nutraceuticals. An exopolysaccharide, Morchella esculenta exopolysaccharide (MEP 
      2), was extracted and purified from the fermentation liquor of M. esculenta. The 
      aim of this study was to investigate its digestion profile, antioxidant capacity, 
      and effect on the microbiota composition in diabetic mice. RESULTS: The study 
      found that MEP 2 was stable during in vitro saliva digestion but was partially 
      degraded during gastric digestion. The digest enzymes exerted a negligible effect 
      on the chemical structure of MEP 2. Molecular weight and atomic force microscope 
      (AFM) images suggest that both smaller chains and larger aggregations were 
      produced. Scanning electron microscope (SEM) images reveal that the surface 
      morphology was much altered after intestinal digestion. After digestion, the 
      antioxidant ability increased as revealed by the 2,2-diphenyl-1-picrylhydrazyl 
      (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. 
      Both MEP 2 and its digested components showed strong alpha-amylase and moderate 
      alpha-glucosidase inhibition activity, leading us to further investigate its ability 
      to modulate the diabetic symptoms. The MEP 2 treatment ameliorated the 
      inflammatory cell infiltration and increased the size of pancreas inlets. Serum 
      concentration of HbA1c was significantly reduced. Blood glucose level during the 
      oral glucose tolerance test (OGTT) was also slightly lower. The MEP 2 increased 
      the diversity of the gut microbiota and modulated the abundance of several 
      important bacteria including Alcaligenaceae, Caulobacteraceae, Prevotella, 
      Brevundimonas, Demequina, and several Lachnospiraceae species. CONCLUSION: It was 
      found that MEP 2 was partially degraded during in vitro digestion. Its potential 
      antidiabetic bioactivity may be associated with its alpha-amylase inhibition and gut 
      microbiome modulation ability. (c) 2023 Society of Chemical Industry.
CI  - (c) 2023 Society of Chemical Industry.
FAU - Wu, Haishan
AU  - Wu H
AD  - School of Food and Biological Engineering, Hefei University of Technology, Hefei, 
      P.R. China.
FAU - Chen, Jing
AU  - Chen J
AD  - Teaching and Research Section of Clinical Nursing, Xiangya Hospital, Central 
      South University, Changsha, China.
AD  - Department of Oral Mucosa, Center of Stomatology, Xiangya Hospital, Central South 
      University, Changsha, China.
FAU - Liu, Yuting
AU  - Liu Y
AD  - School of Food and Biological Engineering, Hefei University of Technology, Hefei, 
      P.R. China.
FAU - Cheng, Haoran
AU  - Cheng H
AD  - School of Food and Biological Engineering, Hefei University of Technology, Hefei, 
      P.R. China.
FAU - Nan, Jian
AU  - Nan J
AD  - School of Food and Biological Engineering, Hefei University of Technology, Hefei, 
      P.R. China.
FAU - Park, Hyun Jin
AU  - Park HJ
AD  - School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of 
      Korea.
FAU - Yang, Liu
AU  - Yang L
AUID- ORCID: 0000-0001-5133-0470
AD  - School of Food and Biological Engineering, Hefei University of Technology, Hefei, 
      P.R. China.
FAU - Li, Jinglei
AU  - Li J
AUID- ORCID: 0000-0001-8814-8672
AD  - School of Food and Biological Engineering, Hefei University of Technology, Hefei, 
      P.R. China.
LA  - eng
GR  - 31700015/National Natural Science Foundation of China/
GR  - 1808085QC66/Natural Science Foundation of Anhui Province/
PT  - Journal Article
DEP - 20230313
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Antioxidants)
RN  - 0 (Hypoglycemic Agents)
RN  - EC 3.2.1.1 (alpha-Amylases)
RN  - Morchella esculenta
SB  - IM
MH  - Animals
MH  - Mice
MH  - Antioxidants/pharmacology/chemistry
MH  - Hypoglycemic Agents/pharmacology
MH  - *Diabetes Mellitus, Experimental/drug therapy
MH  - *Microbiota
MH  - alpha-Amylases
MH  - Digestion
OTO - NOTNLM
OT  - AFM
OT  - DSC
OT  - FTIR
OT  - diabetic mice
OT  - digestion profile
EDAT- 2023/02/23 06:00
MHDA- 2023/06/06 06:42
CRDT- 2023/02/22 12:17
PHST- 2022/12/11 00:00 [revised]
PHST- 2022/01/13 00:00 [received]
PHST- 2023/02/19 00:00 [accepted]
PHST- 2023/06/06 06:42 [medline]
PHST- 2023/02/23 06:00 [pubmed]
PHST- 2023/02/22 12:17 [entrez]
AID - 10.1002/jsfa.12513 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2023 Jul;103(9):4401-4412. doi: 10.1002/jsfa.12513. Epub 2023 
      Mar 13.