PMID- 36807971
OWN - NLM
STAT- MEDLINE
DCOM- 20230410
LR  - 20230410
IS  - 1943-2631 (Electronic)
IS  - 0016-6731 (Linking)
VI  - 223
IP  - 4
DP  - 2023 Apr 6
TI  - Multispecies polyploidization, chromosome shuffling, and genome extraction in 
      Zea/Tripsacum hybrids.
LID - iyad029 [pii]
LID - 10.1093/genetics/iyad029 [doi]
AB  - By hybridization and special sexual reproduction, we sequentially aggregated Zea 
      mays, Zea perennis, and Tripsacum dactyloides in an allohexaploid, backcrossed it 
      with maize, derived self-fertile allotetraploids of maize and Z. perennis by 
      natural genome extraction, extended their first six selfed generations, and 
      finally constructed amphitetraploid maize using nascent allotetraploids as a 
      genetic bridge. Transgenerational chromosome inheritance, subgenome stability, 
      chromosome pairings and rearrangements, and their impacts on an organism's 
      fitness were investigated by fertility phenotyping and molecular cytogenetic 
      techniques genomic in situ hybridization (GISH) and fluorescence in situ 
      hybridization (FISH). Results showed that diversified sexual reproductive methods 
      produced highly differentiated progenies (2n = 35-84) with varying proportions of 
      subgenomic chromosomes, of which one individual (2n = 54, MMMPT) overcame 
      self-incompatibility barriers and produced a self-fertile nascent 
      near-allotetraploid by preferentially eliminating Tripsacum chromosomes. Nascent 
      near-allotetraploid progenies showed persistent chromosome changes, intergenomic 
      translocations, and rDNA variations for at least up to the first six selfed 
      generations; however, the mean chromosome number preferably maintained at the 
      near-tetraploid level (2n = 40) with full integrity of 45S rDNA pairs, and a 
      trend of decreasing variations by advancing generations with an average of 25.53, 
      14.14, and 0.37 for maize, Z. perennis, and T. dactyloides chromosomes, 
      respectively. The mechanisms for three genome stabilities and karyotype evolution 
      for formatting new polyploid species were discussed.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of the 
      Genetics Society of America. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Iqbal, Muhammad Zafar
AU  - Iqbal MZ
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      Wenjiang 611130, Chengdu, Sichuan, China.
FAU - Wen, Xiaodong
AU  - Wen X
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
AD  - Sichuan Rural Science and Technology Development Center, Science and Technology 
      Department of Sichuan Province, Wuhou 610041, Chengdu, Sichuan, China.
FAU - Xu, Lulu
AU  - Xu L
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Zhao, Yanli
AU  - Zhao Y
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Li, Jing
AU  - Li J
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Jiang, Weiming
AU  - Jiang W
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Cheng, Mingjun
AU  - Cheng M
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Li, Huaxiong
AU  - Li H
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Li, Yingzheng
AU  - Li Y
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Li, Xiaofeng
AU  - Li X
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - He, Ruyu
AU  - He R
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - He, Jianmei
AU  - He J
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Su, Yuegui
AU  - Su Y
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Ali, Asif
AU  - Ali A
AD  - Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Peng, Yan
AU  - Peng Y
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      Wenjiang 611130, Chengdu, Sichuan, China.
FAU - Rong, Tingzhao
AU  - Rong T
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Tang, Qilin
AU  - Tang Q
AD  - Maize Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
SB  - IM
MH  - *Zea mays/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Chromosomes, Plant/genetics
MH  - Genome, Plant
MH  - Poaceae/genetics
MH  - Polyploidy
OTO - NOTNLM
OT  - Plant Genetics and Genomics
OT  - chromosome elimination
OT  - homoeologous exchanges
OT  - hybrid speciation
OT  - karyotype evolution
OT  - polyploidy
COIS- Conflicts of interest None declared.
EDAT- 2023/02/23 06:00
MHDA- 2023/04/10 06:42
CRDT- 2023/02/22 12:23
PHST- 2022/12/08 00:00 [received]
PHST- 2023/02/06 00:00 [accepted]
PHST- 2023/04/10 06:42 [medline]
PHST- 2023/02/23 06:00 [pubmed]
PHST- 2023/02/22 12:23 [entrez]
AID - 7048927 [pii]
AID - 10.1093/genetics/iyad029 [doi]
PST - ppublish
SO  - Genetics. 2023 Apr 6;223(4):iyad029. doi: 10.1093/genetics/iyad029.