PMID- 36808352
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20231213
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 27
IP  - 3
DP  - 2023 Feb
TI  - The promoter methylations of the autoimmune regulator (AIRE) gene and matrix 
      metalloproteinase-3 (MMP-3) gene may have a role in gestational diabetes 
      mellitus.
PG  - 1051-1057
LID - 31201 [pii]
LID - 10.26355/eurrev_202302_31201 [doi]
AB  - OBJECTIVE: Gestational diabetes mellitus (GDM) is characterized by new-onset 
      glucose intolerance and is most common in the second and third trimesters of 
      pregnancy. Epigenetic modifications regulate glucose and its cellular 
      interactions with metabolic pathways. Emerging evidence suggests that epigenetic 
      changes contribute to the pathophysiology of GDM. Since these patients have high 
      glucose levels, the metabolic profiles of the fetus and the mother can affect 
      these epigenetic changes. Therefore, we aimed to examine the potential 
      alterations in the methylation profiles of three gene promoters: the autoimmune 
      regulator (AIRE) gene, matrix metalloproteinase-3 (MMP-3), and calcium 
      voltage-gated channel subunit alpha1 G (CACNA1G). PATIENTS AND METHODS: A total 
      of 44 patients diagnosed with GDM and 20 controls were involved in the study. DNA 
      isolation and bisulfite modification were performed from peripheral blood samples 
      of all patients. Then, the promoter methylation status of the AIRE, MMP-3, and 
      CACNA1G genes was determined by methylation-specific polymerase chain reaction 
      (PCR) methylation-specific (MSP). RESULTS: Our results demonstrated that the 
      methylation status of AIRE and MMP-3 changed to unmethylated in the GDM patients 
      compared to healthy pregnant women (p<0.001). However, CACNA1G promoter 
      methylation status failed to show a significant change between experimental 
      groups (p>0.05). CONCLUSIONS: Our results indicated that AIRE and MMP-3 are the 
      genes affected by epigenetic modification, which could be one of the causes of 
      the long-term metabolic effects in maternal and fetal health and can be a target 
      for prevention, diagnosis, or treatment for GDM in future studies.
FAU - Coban, U
AU  - Coban U
AD  - Department of Gynecology and Obstetrics, Faculty of Medicine, Ondokuz Mayis 
      University, Samsun, Turkey. dr.ulascoban@gmail.com.
FAU - Celik, Z B
AU  - Celik ZB
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - IY9XDZ35W2 (Glucose)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.17 (MMP3 protein, human)
SB  - IM
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - *Diabetes, Gestational/metabolism
MH  - DNA Methylation
MH  - Epigenesis, Genetic
MH  - Glucose
MH  - Matrix Metalloproteinase 3/metabolism
MH  - AIRE Protein
EDAT- 2023/02/23 06:00
MHDA- 2023/02/25 06:00
CRDT- 2023/02/22 12:47
PHST- 2023/02/22 12:47 [entrez]
PHST- 2023/02/23 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
AID - 31201 [pii]
AID - 10.26355/eurrev_202302_31201 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2023 Feb;27(3):1051-1057. doi: 
      10.26355/eurrev_202302_31201.