PMID- 36809827
OWN - NLM
STAT- MEDLINE
DCOM- 20230724
LR  - 20230724
IS  - 1638-6183 (Electronic)
IS  - 0300-9084 (Linking)
VI  - 211
DP  - 2023 Aug
TI  - Mycobacterium smegmatis secreting methionine sulfoxide reductase A (MsrA) 
      modulates cellular processes in mouse macrophages.
PG  - 1-15
LID - S0300-9084(23)00048-2 [pii]
LID - 10.1016/j.biochi.2023.02.010 [doi]
AB  - Methionine sulfoxide reductase A (MsrA) is an antioxidant repair enzyme that 
      reduces the oxidized methionine (Met-O) in proteins to methionine (Met). Its 
      pivotal role in the cellular processes has been well established by 
      overexpressing, silencing, and knocking down MsrA or deleting the gene encoding 
      MsrA in several species. We are specifically interested in understanding the role 
      of secreted MsrA in bacterial pathogens. To elucidate this, we infected mouse 
      bone marrow-derived macrophages (BMDMs) with recombinant Mycobacterium smegmatis 
      strain (MSM), secreting a bacterial MsrA or M. smegmatis strain (MSC) carrying 
      only the control vector. BMDMs infected with MSM induced higher levels of ROS and 
      TNF-alpha than BMDMs infected with MSC. The increased ROS and TNF-alpha levels in 
      MSM-infected BMDMs correlated with elevated necrotic cell death in this group. 
      Further, RNA-seq transcriptome analysis of BMDMs infected with MSC and MSM 
      revealed differential expression of protein and RNA coding genes, suggesting that 
      bacterial-delivered MsrA could modulate the host cellular processes. Finally, 
      KEGG pathway enrichment analysis identified the down-regulation of cancer-related 
      signaling genes in MSM-infected cells, indicating that MsrA can potentially 
      regulate the development and progression of cancer.
CI  - Copyright (c) 2023 Elsevier B.V. and Societe Francaise de Biochimie et Biologie 
      Moleculaire (SFBBM). All rights reserved.
FAU - Veerapandian, Raja
AU  - Veerapandian R
AD  - Center of Emphasis in Infectious Diseases, Department of Molecular and 
      Translational Medicine, Paul L. Foster School of Medicine, Texas Tech University 
      Health Sciences Center El Paso, TX, 79905, USA.
FAU - Ramos, Enrique I
AU  - Ramos EI
AD  - Center of Emphasis in Cancer, Paul L. Foster School of Medicine, Department of 
      Molecular and Translational Medicine, Texas Tech University Health Sciences 
      Center El Paso, TX, 79905, USA.
FAU - Vijayaraghavan, Mahalakshmi
AU  - Vijayaraghavan M
AD  - Center of Emphasis in Infectious Diseases, Department of Molecular and 
      Translational Medicine, Paul L. Foster School of Medicine, Texas Tech University 
      Health Sciences Center El Paso, TX, 79905, USA.
FAU - Sedano, Melina J
AU  - Sedano MJ
AD  - Center of Emphasis in Cancer, Paul L. Foster School of Medicine, Department of 
      Molecular and Translational Medicine, Texas Tech University Health Sciences 
      Center El Paso, TX, 79905, USA.
FAU - Carmona, Areanna
AU  - Carmona A
AD  - Center of Emphasis in Infectious Diseases, Department of Molecular and 
      Translational Medicine, Paul L. Foster School of Medicine, Texas Tech University 
      Health Sciences Center El Paso, TX, 79905, USA.
FAU - Chacon, Jessica A
AU  - Chacon JA
AD  - Department of Medical Education, Paul L. Foster School of Medicine, Texas Tech 
      University Health Sciences Center El Paso, TX, 79905, USA.
FAU - Gadad, Shrikanth S
AU  - Gadad SS
AD  - Center of Emphasis in Cancer, Paul L. Foster School of Medicine, Department of 
      Molecular and Translational Medicine, Texas Tech University Health Sciences 
      Center El Paso, TX, 79905, USA; Frederick L. Francis Graduate School of 
      Biomedical Sciences, Texas Tech University Health Sciences Center El Paso, Texas, 
      79905, USA; Mays Cancer Center, UT Health San Antonio MD Anderson Cancer Center, 
      San Antonio, TX, 78229, USA. Electronic address: shrikanth.gadad@ttuhsc.edu.
FAU - Dhandayuthapani, Subramanian
AU  - Dhandayuthapani S
AD  - Center of Emphasis in Infectious Diseases, Department of Molecular and 
      Translational Medicine, Paul L. Foster School of Medicine, Texas Tech University 
      Health Sciences Center El Paso, TX, 79905, USA; Frederick L. Francis Graduate 
      School of Biomedical Sciences, Texas Tech University Health Sciences Center El 
      Paso, Texas, 79905, USA. Electronic address: s.dhandayuthapani@ttuhsc.edu.
LA  - eng
PT  - Journal Article
DEP - 20230219
PL  - France
TA  - Biochimie
JT  - Biochimie
JID - 1264604
RN  - AE28F7PNPL (Methionine)
RN  - EC 1.8.4.- (Methionine Sulfoxide Reductases)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Macrophages/microbiology
MH  - Methionine/metabolism
MH  - *Methionine Sulfoxide Reductases/genetics/metabolism
MH  - *Mycobacterium smegmatis/enzymology/genetics
MH  - Oxidative Stress
MH  - Reactive Oxygen Species/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - MsrA
OT  - Mycobacterium smegmatis
OT  - Necrosis
OT  - RNA-Seq
OT  - ROS
OT  - Secretion
OT  - Transcriptome
COIS- Declaration of competing interest "The authors declare no conflict of interest."
EDAT- 2023/02/23 06:00
MHDA- 2023/07/10 06:42
CRDT- 2023/02/22 14:11
PHST- 2022/11/15 00:00 [received]
PHST- 2023/02/16 00:00 [revised]
PHST- 2023/02/17 00:00 [accepted]
PHST- 2023/07/10 06:42 [medline]
PHST- 2023/02/23 06:00 [pubmed]
PHST- 2023/02/22 14:11 [entrez]
AID - S0300-9084(23)00048-2 [pii]
AID - 10.1016/j.biochi.2023.02.010 [doi]
PST - ppublish
SO  - Biochimie. 2023 Aug;211:1-15. doi: 10.1016/j.biochi.2023.02.010. Epub 2023 Feb 
      19.