PMID- 36811153
OWN - NLM
STAT- MEDLINE
DCOM- 20240422
LR  - 20240422
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 602
IP  - 8
DP  - 2024 Apr
TI  - Fast and sensitive GCaMP calcium indicators for neuronal imaging.
PG  - 1595-1604
LID - 10.1113/JP283832 [doi]
AB  - We review the principles of development and deployment of genetically encoded 
      calcium indicators (GECIs) for the detection of neural activity. Our focus is on 
      the popular GCaMP family of green GECIs, culminating in the recent release of the 
      jGCaMP8 sensors, with dramatically improved kinetics relative to previous 
      generations. We summarize the properties of GECIs in multiple colour channels 
      (blue, cyan, green, yellow, red, far-red) and highlight areas for further 
      improvement. With their low-millisecond rise-times, the jGCaMP8 indicators allow 
      new classes of experiments following neural activity in time frames approaching 
      the underlying computations.
CI  - (c) 2023 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd 
      on behalf of The Physiological Society.
FAU - Zhang, Yan
AU  - Zhang Y
AUID- ORCID: 0000-0001-8376-3190
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
FAU - Looger, Loren L
AU  - Looger LL
AD  - Department of Neurosciences, Howard Hughes Medical Institute, University of 
      California, San Diego, La Jolla, CA, USA.
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Review
DEP - 20230314
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - *Calcium/metabolism
MH  - *Neurons/physiology
MH  - Diagnostic Imaging
MH  - Calcium Signaling
OTO - NOTNLM
OT  - GCaMP
OT  - calcium sensors
OT  - genetically encoded calcium indicator (GECI)
OT  - neuronal imaging
EDAT- 2023/02/23 06:00
MHDA- 2024/04/22 06:43
CRDT- 2023/02/22 15:17
PHST- 2022/11/20 00:00 [received]
PHST- 2023/02/15 00:00 [accepted]
PHST- 2024/04/22 06:43 [medline]
PHST- 2023/02/23 06:00 [pubmed]
PHST- 2023/02/22 15:17 [entrez]
AID - 10.1113/JP283832 [doi]
PST - ppublish
SO  - J Physiol. 2024 Apr;602(8):1595-1604. doi: 10.1113/JP283832. Epub 2023 Mar 14.