PMID- 36813560
OWN - NLM
STAT- Publisher
LR  - 20240216
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
DP  - 2023 Feb 22
TI  - Spatial resolution of renal amyloid deposits through MALDI-MSI: a combined 
      digital and molecular approach to monoclonal gammopathies.
LID - jcp-2023-208790 [pii]
LID - 10.1136/jcp-2023-208790 [doi]
AB  - AIMS: Identification and characterisation of monoclonal gammopathies of renal 
      significance (MGRS) is critical for therapeutic purposes. Amyloidosis represents 
      one of the most common forms of MGRS, and renal biopsy remains the gold standard 
      for their classification, although mass spectrometry has shown greater 
      sensitivity in this area. METHODS: In the present study, a new in situ proteomic 
      technique, matrix-assisted laser desorption/ionisation mass spectrometry imaging 
      (MALDI-MSI), is investigated as an alternative to conventional laser capture 
      microdissection MS for the characterisation of amyloids. MALDI-MSI was performed 
      on 16 cases (3 lambda light chain amyloidosis (AL), 3 AL kappa, 3 serum amyloid A 
      amyloidosis (SAA), 2 lambda light chain deposition disease (LCDD), 2 challenging 
      amyloid cases and 3 controls). Analysis began with regions of interest labelled 
      by the pathologist, and then automatic segmentation was performed. RESULTS: 
      MALDI-MSI correctly identified and typed cases with known amyloid type (AL kappa, 
      AL lambda and SAA). A 'restricted fingerprint' for amyloid detection composed of 
      apolipoprotein E, serum amyloid protein and apolipoprotein A1 showed the best 
      automatic segmentation performance (area under the curve >0.7). CONCLUSIONS: 
      MALDI-MSI correctly assigned minimal/challenging cases of amyloidosis to the 
      correct type (AL lambda) and identified lambda light chains in LCDD cases, 
      highlighting the promising role of MALDI-MSI for amyloid typing.
CI  - (c) Author(s) (or their employer(s)) 2023. No commercial re-use. See rights and 
      permissions. Published by BMJ.
FAU - Bindi, Greta
AU  - Bindi G
AD  - Department of Medicine and Surgery, Proteomics and Metabolomics Units, University 
      of Milano-Bicocca, Monza, Italy.
FAU - Smith, Andrew
AU  - Smith A
AD  - Department of Medicine and Surgery, Proteomics and Metabolomics Units, University 
      of Milano-Bicocca, Monza, Italy.
FAU - Oliveira, Glenda
AU  - Oliveira G
AD  - Department of Medicine and Surgery, Proteomics and Metabolomics Units, University 
      of Milano-Bicocca, Monza, Italy.
FAU - Eccher, Albino
AU  - Eccher A
AD  - Department of Pathology and Diagnostics, University and Hospital Trust of Verona, 
      Verona, Italy.
FAU - Vatrano, Simona
AU  - Vatrano S
AD  - Pathology Unit, ASP Catania, "Gravina" Hospital, Caltagirone, Italy.
FAU - Alberici, Federico
AU  - Alberici F
AD  - Nephrology Unit, Spedali Civili Hospital, ASST Spedali Civili di Brescia, 
      Brescia, Italy.
FAU - Cazzaniga, Giorgio
AU  - Cazzaniga G
AD  - Department of Medicine and Surgery, Pathology, University of Milano-Bicocca, 
      IRCCS Fondazione San Gerardo dei Tintori, Monza, Italy.
FAU - Galimberti, Stefania
AU  - Galimberti S
AD  - Bicocca Bioinformatics Biostatistics and Bioimaging B4 Center, School of Medicine 
      and Surgery, University of Milano-Bicocca, Monza, Italy.
FAU - Capitoli, Giulia
AU  - Capitoli G
AD  - Bicocca Bioinformatics Biostatistics and Bioimaging B4 Center, School of Medicine 
      and Surgery, University of Milano-Bicocca, Monza, Italy.
FAU - Magni, Fulvio
AU  - Magni F
AD  - Department of Medicine and Surgery, Proteomics and Metabolomics Units, University 
      of Milano-Bicocca, Monza, Italy.
FAU - Pagni, Fabio
AU  - Pagni F
AUID- ORCID: 0000-0001-9625-1637
AD  - Department of Medicine and Surgery, Pathology, University of Milano-Bicocca, 
      IRCCS Fondazione San Gerardo dei Tintori, Monza, Italy.
FAU - L'Imperio, Vincenzo
AU  - L'Imperio V
AUID- ORCID: 0000-0002-9284-2998
AD  - Department of Medicine and Surgery, Pathology, University of Milano-Bicocca, 
      IRCCS Fondazione San Gerardo dei Tintori, Monza, Italy 
      vincenzo.limperio@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20230222
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
SB  - IM
OTO - NOTNLM
OT  - amyloid
OT  - glomerulonephritis
OT  - hematology
COIS- Competing interests: None declared.
EDAT- 2023/02/23 06:00
MHDA- 2023/02/23 06:00
CRDT- 2023/02/22 21:42
PHST- 2023/01/17 00:00 [received]
PHST- 2023/02/10 00:00 [accepted]
PHST- 2023/02/22 21:42 [entrez]
PHST- 2023/02/23 06:00 [pubmed]
PHST- 2023/02/23 06:00 [medline]
AID - jcp-2023-208790 [pii]
AID - 10.1136/jcp-2023-208790 [doi]
PST - aheadofprint
SO  - J Clin Pathol. 2023 Feb 22:jcp-2023-208790. doi: 10.1136/jcp-2023-208790.