PMID- 36816753
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240302
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 13
IP  - 3
DP  - 2023 Mar
TI  - KLF regulation of insulin pathway genes.
PG  - 87
LID - 10.1007/s13205-023-03502-5 [doi]
LID - 87
AB  - Alteration in lipid metabolism can result in fat accumulation in adipose tissues, 
      which may lead to two most important human diseases, obesity and diabetes. A 
      shift in lipid metabolism deregulates signaling pathways which regulates obesity 
      and/or diabetes. In this study, we examined the components of insulin/ TGF-beta 
      pathways and their genetic interaction with Kruppel-like transcription factors 
      (KLFs). Their role in energy homeostasis were discussed. We separately created 
      klf/daf genes double mutants by carrying out klfs RNAi on daf-2 (e1391), daf-4 
      (e1364), daf-7 (e1372); dpy-1 (e1), daf-14 (m77), daf-16 (mgDf50) mutants. And 
      then conducted Oil O Red staining to assay the klf/daf RNAi worms for fat 
      deposits and examine genetic interaction between klfs and daf genes. The results 
      showed that worms bearing klf-1, 2, or 3 and daf-2, or daf-4 mutations deposit 
      large, but similar fat levels as individual mutants. The results suggested that 
      they target the same molecular pathway of fat storage. klf-1, 2 or 3 RNAi /daf-7 
      worms showed higher fat deposits in klf-1, 2, or 3 RNAi/daf-7 worms than klf-1, 
      2, or 3 RNAi or daf-7 mutants alone, which showed a functional interaction 
      between klfs and daf-7 in perhaps TGF-beta-like pathway. Altogether our study 
      suggests a direct role of klfs in insulin signaling pathway.
CI  - (c) King Abdulaziz City for Science and Technology 2023, Springer Nature or its 
      licensor (e.g. a society or other partner) holds exclusive rights to this article 
      under a publishing agreement with the author(s) or other rightsholder(s); author 
      self-archiving of the accepted manuscript version of this article is solely 
      governed by the terms of such publishing agreement and applicable law.
FAU - Wang, Huan
AU  - Wang H
AUID- ORCID: 0000-0001-5976-3537
AD  - College of Bioscience and Biotechnology, Shenyang Agriculture University, 
      Shanyang, 110866 China. GRID: grid.412557.0. ISNI: 0000 0000 9886 8131
AD  - Rutgers Center for Lipid Research, New Jersey Institute for Food, Nutrition, and 
      Health, Rutgers University, New Brunswick, USA. GRID: grid.430387.b. ISNI: 0000 
      0004 1936 8796
FAU - Brey, Christopher W
AU  - Brey CW
AD  - Science Department, Marywood University, Scranton, PA 18505 USA. GRID: 
      grid.259706.f. ISNI: 0000 0000 8681 4777
FAU - Wang, Yi
AU  - Wang Y
AD  - Laboratory of Developmental Biology, Center for Vector Biology, Rutgers 
      University, 180 Jones Avenue, New Brunswick, NJ 08901 USA. GRID: grid.430387.b. 
      ISNI: 0000 0004 1936 8796
FAU - Gaugler, Randy
AU  - Gaugler R
AD  - Laboratory of Developmental Biology, Center for Vector Biology, Rutgers 
      University, 180 Jones Avenue, New Brunswick, NJ 08901 USA. GRID: grid.430387.b. 
      ISNI: 0000 0004 1936 8796
FAU - Hashmi, Sarwar
AU  - Hashmi S
AD  - Laboratory of Developmental Biology, Center for Vector Biology, Rutgers 
      University, 180 Jones Avenue, New Brunswick, NJ 08901 USA. GRID: grid.430387.b. 
      ISNI: 0000 0004 1936 8796
AD  - Rutgers Center for Lipid Research, New Jersey Institute for Food, Nutrition, and 
      Health, Rutgers University, New Brunswick, USA. GRID: grid.430387.b. ISNI: 0000 
      0004 1936 8796
LA  - eng
PT  - Journal Article
DEP - 20230216
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC9935763
OTO - NOTNLM
OT  - Caenorhabditis elegans
OT  - Insulin pathway
OT  - Kruppel-like transcription factors
OT  - TGF beta pathway
OT  - daf
OT  - klf
COIS- Conflict of interestAll the authors declare that they are no conflict of 
      interest.
EDAT- 2023/02/24 06:00
MHDA- 2023/02/24 06:01
PMCR- 2024/03/01
CRDT- 2023/02/23 09:26
PHST- 2022/10/27 00:00 [received]
PHST- 2023/01/26 00:00 [accepted]
PHST- 2023/02/23 09:26 [entrez]
PHST- 2023/02/24 06:00 [pubmed]
PHST- 2023/02/24 06:01 [medline]
PHST- 2024/03/01 00:00 [pmc-release]
AID - 3502 [pii]
AID - 10.1007/s13205-023-03502-5 [doi]
PST - ppublish
SO  - 3 Biotech. 2023 Mar;13(3):87. doi: 10.1007/s13205-023-03502-5. Epub 2023 Feb 16.