PMID- 36817038
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230224
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Print)
IS  - 1792-1074 (Linking)
VI  - 25
IP  - 3
DP  - 2023 Mar
TI  - Heterogeneity of adherent and non‑adherent JAWS II dendritic cells.
PG  - 93
LID - 10.3892/ol.2023.13680 [doi]
LID - 93
AB  - Dendritic cells (DCs) are crucial in the development of immune responses. DC JAWS 
      II is a murine cell line frequently used in DC studies. These cells are grown in 
      two cell fractions: Adherent and non-adherent. The present study aimed to compare 
      these two fractions in both immature and lipopolysaccharide (LPS)-activated JAWS 
      II cells. The present study analysed the condition, phenotype, antigen uptake 
      capability, signalling properties and the influence on the activity of T cells 
      using flow cytometry, mixed cell reaction and ELISA methods. Adherent immature 
      JAWS II cells exhibited increased endocytosis and decreased activation of the 
      Pi3K signalling pathway. After LPS activation, adherent JAWS II cells exhibited 
      increased expression levels of CD80 and CD86 costimulatory molecules, increased 
      endocytosis and an elevated ability to induce T cell proliferation, compared with 
      non-adherent cells. These results demonstrated that the two fractions of JAWS II 
      adherent and non-adherent cells exhibited different properties and this should be 
      taken into account in the planning of research.
CI  - Copyright (c) 2023, Spandidos Publications.
FAU - Maruszewska-Cheruiyot, Marta
AU  - Maruszewska-Cheruiyot M
AD  - Department of Experimental Immunotherapy, Faculty of Medicine, Lazarski 
      University, 02-662 Warsaw, Poland.
FAU - Machcinska, Maja
AU  - Machcinska M
AD  - Department of Experimental Immunotherapy, Faculty of Medicine, Lazarski 
      University, 02-662 Warsaw, Poland.
FAU - Kierasinska, Magdalena
AU  - Kierasinska M
AD  - Laboratory of Parasitology, General Karol Kaczkowski Military Institute of 
      Hygiene and Epidemiology, 01-163 Warsaw, Poland.
FAU - Donskow-Lysoniewska, Katarzyna
AU  - Donskow-Lysoniewska K
AD  - Department of Experimental Immunotherapy, Faculty of Medicine, Lazarski 
      University, 02-662 Warsaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20230125
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC9932576
OTO - NOTNLM
OT  - DCs
OT  - JAWS II
OT  - adherent
OT  - lipopolysaccharide
OT  - non-adherent
COIS- The authors declare that they have no competing interests.
EDAT- 2023/02/24 06:00
MHDA- 2023/02/24 06:01
PMCR- 2023/01/25
CRDT- 2023/02/23 09:30
PHST- 2022/09/08 00:00 [received]
PHST- 2022/11/08 00:00 [accepted]
PHST- 2023/02/23 09:30 [entrez]
PHST- 2023/02/24 06:00 [pubmed]
PHST- 2023/02/24 06:01 [medline]
PHST- 2023/01/25 00:00 [pmc-release]
AID - OL-25-3-13680 [pii]
AID - 10.3892/ol.2023.13680 [doi]
PST - epublish
SO  - Oncol Lett. 2023 Jan 25;25(3):93. doi: 10.3892/ol.2023.13680. eCollection 2023 
      Mar.