PMID- 36820311
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230224
IS  - 2694-2437 (Electronic)
IS  - 2694-2437 (Linking)
VI  - 3
IP  - 1
DP  - 2023 Feb 15
TI  - Platelet Response to Allergens, CXCL10, and CXCL5 in the Context of Asthma.
PG  - 87-96
LID - 10.1021/acsbiomedchemau.2c00059 [doi]
AB  - Asthma is a chronic respiratory disease initiated by a variety of factors, 
      including allergens. During an asthma attack, the secretion of C-X-C-motif 
      chemokine 10 (CXCL10) and chemokine ligand 5 (CCL5) causes the migration of 
      immune cells, including platelets, into the lungs and airway. Platelets, which 
      contain three classes of chemical messenger-filled granules, can secrete 
      vasodilators (adenosine diphosphate and adenosine triphosphate), serotonin (a 
      vasoconstrictor and a vasodilator, depending on the biological system), 
      platelet-activating factor, N-formylmethionyl-leucyl-phenylalanine ((fMLP), a 
      bacterial tripeptide that stimulates chemotaxis), and chemokines (CCL5, platelet 
      factor 4 (PF4), and C-X-C-motif chemokine 12 (CXCL12)), amplifying the asthma 
      response. The goal of this work was threefold: (1) to understand if and how the 
      antibody immunoglobulin E (IgE), responsible for allergic reactions, affects 
      platelet response to the common platelet activator thrombin; (2) to understand 
      how allergen stimulation compares to thrombin stimulation; and (3) to monitor 
      platelet response to fMLP and the chemokines CXCL10 and CCL5. Herein, 
      high-pressure liquid chromatography with electrochemical detection and/or 
      carbon-fiber microelectrode amperometry measured granular secretion events from 
      platelets with and without IgE in the presence of the allergen 
      2,4,6-trinitrophenyl-conjugated ovalbumin (TNP-Ova), thrombin, CXCL10, or CCL5. 
      Platelet adhesion and chemotaxis were measured using a microfluidic platform in 
      the presence of CXCL10, CCL5, or TNP-OVA. Results indicate that IgE binding 
      promotes delta-granule secretion in response to platelet stimulation by thrombin in 
      bulk. Single-cell results on platelets with exogenous IgE exposure showed 
      significant changes in the post-membrane-granule fusion behavior during chemical 
      messenger delivery events after thrombin stimulation. In addition, TNP-Ova 
      allergen stimulation of IgE-exposed platelets secreted serotonin to the same 
      extent as thrombin platelet stimulation. Enhanced adhesion to endothelial cells 
      was demonstrated by TNP-Ova stimulation. Finally, only after incubation with IgE 
      did platelets secrete chemical messengers in response to stimulation with fMLP, 
      CXCL10, and CCL5.
CI  - (c) 2022 The Authors. Published by American Chemical Society.
FAU - Gruba, Sarah
AU  - Gruba S
AD  - Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, 
      United States.
FAU - Wu, Xiaojie
AU  - Wu X
AD  - Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, 
      United States.
FAU - Spanolios, Eleni
AU  - Spanolios E
AD  - Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, 
      United States.
FAU - He, Jiayi
AU  - He J
AD  - Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, 
      United States.
FAU - Xiong-Hang, Kang
AU  - Xiong-Hang K
AD  - Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, 
      United States.
FAU - Haynes, Christy L
AU  - Haynes CL
AUID- ORCID: 0000-0002-5420-5867
AD  - Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, 
      United States.
LA  - eng
PT  - Journal Article
DEP - 20221202
PL  - United States
TA  - ACS Bio Med Chem Au
JT  - ACS bio & med chem Au
JID - 9918232604406676
PMC - PMC9936497
COIS- The authors declare no competing financial interest.
EDAT- 2023/02/24 06:00
MHDA- 2023/02/24 06:01
PMCR- 2022/12/02
CRDT- 2023/02/23 10:36
PHST- 2022/09/14 00:00 [received]
PHST- 2022/11/15 00:00 [revised]
PHST- 2022/11/16 00:00 [accepted]
PHST- 2023/02/23 10:36 [entrez]
PHST- 2023/02/24 06:00 [pubmed]
PHST- 2023/02/24 06:01 [medline]
PHST- 2022/12/02 00:00 [pmc-release]
AID - 10.1021/acsbiomedchemau.2c00059 [doi]
PST - epublish
SO  - ACS Bio Med Chem Au. 2022 Dec 2;3(1):87-96. doi: 10.1021/acsbiomedchemau.2c00059. 
      eCollection 2023 Feb 15.