PMID- 36821035
OWN - NLM
STAT- MEDLINE
DCOM- 20230721
LR  - 20230722
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 30
IP  - 8
DP  - 2023 Aug
TI  - Oxidative Stress and Cell Cycle Arrest in Seminiferous Tubules Nearby Varicose 
      Vessels: New Perspectives from Experimental Varicocele.
PG  - 2401-2415
LID - 10.1007/s43032-023-01200-4 [doi]
AB  - Varicocele (VCL) has been shown to induce severe oxidative stress in the 
      testicular tissue resulting in 35% of males with primary infertility. To compare 
      the exacerbating impacts of varicose on oxidative DNA damage and homeostatic 
      antioxidant reactions in the seminiferous tubules (ST), enclosed and far from 
      varicose vessels. Thirty mature Wistar rats were divided into control and 
      VCL-induced groups. To approve VCL, the testicular diameters, volume, and blood 
      circulation were measured using B-mode and Doppler ultrasonography. Next, to 
      confirm oxidative stress (OS), the global homeostatic antioxidant biomarkers were 
      evaluated. Moreover, the OS-induced oxidative DNA damage and homeostatic 
      antioxidant reactions were compared between STs nearby and far from varicose 
      vessels. Finally, to clarify the DNA damage-induced impact on the cell cycle 
      progression, the global and local expressions of Cyclin D1, Cdk4, and p21 were 
      examined. The VCL-induced group exhibited diminished global antioxidant status 
      (marked with TAC, GPX, SOD, and CAT) and UNG and MPG expression levels. Moreover, 
      the cross-sections of the VCL group represented a prominent reduction in the UNG, 
      MPG, Cyclin D1, and cdk4, and upregulation in the p21 expression levels, more 
      prominently in the STs nearby varicose vessels. Concerning severe oxidative DNA 
      damage and intensive molecular changes in the STs nearby the varicose vessels, 
      they can be considered the main cause of oxidative DNA damage in enclosed 
      tubules. Thus, the varicose-mediated oxidative DNA damage negatively impacts the 
      cell cycle progression in the tubules more intensively in the subcapsular area.
CI  - (c) 2023. The Author(s), under exclusive licence to Society for Reproductive 
      Investigation.
FAU - Minas, Aram
AU  - Minas A
AUID- ORCID: 0000-0001-9763-1041
AD  - Department of Surgery, Division of Urology, Human Reproduction Section, 
      Universidade Federal de Sao Paulo- UNIFESP, Sao Paulo, Brazil.
FAU - Razi, Mazdak
AU  - Razi M
AUID- ORCID: 0000-0002-4309-4831
AD  - Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, 
      Urmia, Iran. Mazdak.razi@gmail.com.
FAU - Nasr-Esfahani, Mohammad Hossein
AU  - Nasr-Esfahani MH
AUID- ORCID: 0000-0003-1983-3435
AD  - Department of Animal Biotechnology, Jahad Daneshgahi Biotechnology Research 
      Institute, Royan Research Institute, Reproductive Medical Research Center, 
      Isfahan, Iran.
FAU - Hashemi-Asl, Seyed Mohammad
AU  - Hashemi-Asl SM
AUID- ORCID: 0000-0003-3909-2144
AD  - Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, 
      Urmia University, Urmia, Iran.
FAU - Tavalaee, Marziyeh
AU  - Tavalaee M
AUID- ORCID: 0000-0001-9954-964X
AD  - Department of Animal Biotechnology, Jahad Daneshgahi Biotechnology Research 
      Institute, Royan Research Institute, Reproductive Medical Research Center, 
      Isfahan, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230223
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (Antioxidants)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Rats
MH  - Male
MH  - Humans
MH  - Animals
MH  - *Antioxidants/pharmacology
MH  - *Varicocele/metabolism
MH  - Cyclin D1/metabolism
MH  - Rats, Wistar
MH  - Testis/metabolism
MH  - Oxidative Stress
MH  - Seminiferous Tubules/metabolism
MH  - Cell Cycle Checkpoints
OTO - NOTNLM
OT  - Cell cycle
OT  - DNA repairmen
OT  - Oxidative stress
OT  - Varicocele
OT  - Varicose vessels
EDAT- 2023/02/24 06:00
MHDA- 2023/07/21 06:43
CRDT- 2023/02/23 11:36
PHST- 2022/11/08 00:00 [received]
PHST- 2023/02/08 00:00 [accepted]
PHST- 2023/07/21 06:43 [medline]
PHST- 2023/02/24 06:00 [pubmed]
PHST- 2023/02/23 11:36 [entrez]
AID - 10.1007/s43032-023-01200-4 [pii]
AID - 10.1007/s43032-023-01200-4 [doi]
PST - ppublish
SO  - Reprod Sci. 2023 Aug;30(8):2401-2415. doi: 10.1007/s43032-023-01200-4. Epub 2023 
      Feb 23.