PMID- 36824367
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230226
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 11
DP  - 2023
TI  - miR-514a promotes neuronal development in human iPSC-derived neurons.
PG  - 1096463
LID - 10.3389/fcell.2023.1096463 [doi]
LID - 1096463
AB  - Proper development and function of the central nervous system require precise 
      regulation of gene expression. MicroRNAs (miRNAs), a group of small non-coding 
      RNAs that can negatively regulate gene expression at the post-transcriptional 
      level, are critical regulators of neuronal development, and dysregulation of 
      microRNAs has been implicated in various neurological disorders. Changes in 
      microRNA expression and repertoire are related to the emergence of social and 
      behavioral variations in closely related primates, including humans, during 
      evolution. MicroRNA-514a (miR-514a) is an X-linked miRNA that is conserved in 
      species with higher social and cognitive functions, and frequent tandem 
      duplications of miR-514a have been found in primate genomes. Here, we demonstrate 
      that miR-514a plays a crucial role in neuronal development in neurons derived 
      from human induced pluripotent stem cells (iPSCs). Overexpression of miR-514a 
      increased dendritic length, soma size, and activity levels of mammalian target of 
      rapamycin (mTOR) signaling in induced pluripotent stem cell-derived neurons, 
      whereas blocking of endogenous miR-514a inhibited neuronal development. 
      Furthermore, we performed a functional analysis of the miR-514a variation found 
      during primate evolution, to investigate the impact of miR-514a sequence 
      variation and associated changes in expression on brain development during 
      evolution. We found that mutation in miR-514a significantly reduced the 
      expression of the mature form and abolished the effects observed when native 
      miR-514a was expressed. Our findings provide new insights into the functional 
      role of miR-514a in the regulation of neuronal development and evolution of 
      primate brain development.
CI  - Copyright (c) 2023 Akaba, Takahashi, Suzuki, Kosaki and Tsujimura.
FAU - Akaba, Yuichi
AU  - Akaba Y
AD  - Group of Brain Function and Development, Neuroscience Institute of the Graduate 
      School of Science, Nagoya University, Nagoya, Aichi, Japan.
AD  - Research Unit for Developmental Disorders, Institute for Advanced Research, 
      Nagoya University, Nagoya, Aichi, Japan.
AD  - Department of Pediatrics, Asahikawa Medical University, Asahikawa, Hokkaido, 
      Japan.
FAU - Takahashi, Satoru
AU  - Takahashi S
AD  - Department of Pediatrics, Asahikawa Medical University, Asahikawa, Hokkaido, 
      Japan.
FAU - Suzuki, Keiichiro
AU  - Suzuki K
AD  - Institute for Advanced Co-Creation Studies, Osaka University, Osaka, Japan.
AD  - Graduate School of Engineering Science, Osaka University, Osaka, Japan.
AD  - Graduate School of Frontier Bioscience, Osaka University, Osaka, Japan.
FAU - Kosaki, Kenjiro
AU  - Kosaki K
AD  - Center for Medical Genetics, Keio University School of Medicine, Tokyo, Japan.
FAU - Tsujimura, Keita
AU  - Tsujimura K
AD  - Group of Brain Function and Development, Neuroscience Institute of the Graduate 
      School of Science, Nagoya University, Nagoya, Aichi, Japan.
AD  - Research Unit for Developmental Disorders, Institute for Advanced Research, 
      Nagoya University, Nagoya, Aichi, Japan.
LA  - eng
PT  - Journal Article
DEP - 20230207
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC9941156
OTO - NOTNLM
OT  - IPSC
OT  - dendrite
OT  - mTOR
OT  - miR-514a
OT  - microRNA
OT  - neuronal development
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as potential 
      conflicts of interest.
EDAT- 2023/02/25 06:00
MHDA- 2023/02/25 06:01
PMCR- 2023/01/01
CRDT- 2023/02/24 02:38
PHST- 2022/11/12 00:00 [received]
PHST- 2023/01/26 00:00 [accepted]
PHST- 2023/02/24 02:38 [entrez]
PHST- 2023/02/25 06:00 [pubmed]
PHST- 2023/02/25 06:01 [medline]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1096463 [pii]
AID - 10.3389/fcell.2023.1096463 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2023 Feb 7;11:1096463. doi: 10.3389/fcell.2023.1096463. 
      eCollection 2023.