PMID- 36827092
OWN - NLM
STAT- MEDLINE
DCOM- 20231113
LR  - 20240112
IS  - 1550-9109 (Electronic)
IS  - 0161-8105 (Linking)
VI  - 46
IP  - 11
DP  - 2023 Nov 8
TI  - Microglial voltage-dependent anion channel 1 signaling modulates sleep 
      deprivation-induced transition to chronic postsurgical pain.
LID - zsad039 [pii]
LID - 10.1093/sleep/zsad039 [doi]
AB  - STUDY OBJECTIVES: This study verified that sleep deprivation before and after 
      skin/muscle incision and retraction (SMIR) surgery increased the risk of chronic 
      pain and investigated the underlying roles of microglial voltage-dependent anion 
      channel 1 (VDAC1) signaling. METHODS: Adult mice received 6 hours of total sleep 
      deprivation from 1 day prior to SMIR until the third day after surgery. 
      Mechanical and heat-evoked pain was assessed before and within 21 days after 
      surgery. Microglial activation and changes in VDAC1 expression and 
      oligomerization were measured. Minocycline was injected to observe the effects of 
      inhibiting microglial activation on pain maintenance. The VDAC1 inhibitor 
      4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) and oligomerization 
      inhibitor VBIT-4 were used to determine the roles of VDAC1 signaling on 
      microglial adenosine 5' triphosphate (ATP) release, inflammation (IL-1beta and 
      CCL2), and chronicity of pain. RESULTS: Sleep deprivation significantly increased 
      the pain duration after SMIR surgery, activated microglia, and enhanced VDAC1 
      signaling in the spinal cord. Minocycline inhibited microglial activation and 
      alleviated sleep deprivation-induced pain maintenance. Lipopolysaccharide 
      (LPS)-induced microglial activation was accompanied by increased VDAC1 expression 
      and oligomerization, and more VDAC1 was observed on the cell membrane surface 
      compared with control. DIDS and VBIT-4 rescued LPS-induced microglial ATP release 
      and IL-1beta and CCL2 expression. DIDS and VBIT-4 reversed sleep loss-induced 
      microglial activation and pain chronicity in mice, similar to the effects of 
      minocycline. No synergistic effects were found for minocycline plus VBIT-4 or 
      DIDS. CONCLUSIONS: Perioperative sleep deprivation activated spinal microglia and 
      increases the risk of chronic postsurgical pain in mice. VDAC1 signaling 
      regulates microglial activation-related ATP release, inflammation, and chronicity 
      of pain.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of Sleep 
      Research Society. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Wei, Shi-Nan
AU  - Wei SN
AD  - The Postgraduate Training Base of Jinzhou Medical University, Beijing, China.
AD  - Department of Anesthesiology, The PLA Rocket Force Characteristic Medical Center, 
      Beijing, China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Department of Anesthesiology, The PLA Rocket Force Characteristic Medical Center, 
      Beijing, China.
FAU - Lu, Yan
AU  - Lu Y
AD  - Department of Neurology, The PLA Rocket Force Characteristic Medical Center, 
      Beijing, China.
FAU - Yu, Hui-Jie
AU  - Yu HJ
AD  - Department of Nuclear Radiation Injury and Monitoring, The PLA Rocket Force 
      Characteristic Medical Center, Beijing, China.
FAU - Ma, Tao
AU  - Ma T
AD  - Department of Anesthesiology, The PLA Rocket Force Characteristic Medical Center, 
      Beijing, China.
FAU - Wang, Si-Nian
AU  - Wang SN
AD  - Department of Nuclear Radiation Injury and Monitoring, The PLA Rocket Force 
      Characteristic Medical Center, Beijing, China.
FAU - Yang, Kun
AU  - Yang K
AD  - The Postgraduate Training Base of Jinzhou Medical University, Beijing, China.
FAU - Tian, Mou-Li
AU  - Tian ML
AD  - Department of Anesthesiology, Changzheng Hospital Affiliate to the Naval Medical 
      University, Shanghai, China.
FAU - Huang, Ai-Hua
AU  - Huang AH
AD  - Department of Neurology, The PLA Rocket Force Characteristic Medical Center, 
      Beijing, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Anesthesiology, The PLA Rocket Force Characteristic Medical Center, 
      Beijing, China.
FAU - Li, Feng-Sheng
AU  - Li FS
AD  - Department of Nuclear Radiation Injury and Monitoring, The PLA Rocket Force 
      Characteristic Medical Center, Beijing, China.
FAU - Li, Yong-Wang
AU  - Li YW
AD  - Department of Anesthesiology, The Third people's Hospital of Longgang District, 
      Shenzhen, China.
LA  - eng
GR  - 82171203/National Natural Science Foundation of China/
GR  - AEP17J001/CPLA Key Project for Medical Sciences/
GR  - LGKCYLWS2021000034/Longgang District Science and Technology Development 
      Foundation of Shenzhen/
PT  - Journal Article
PL  - United States
TA  - Sleep
JT  - Sleep
JID - 7809084
RN  - EC 1.6.- (Voltage-Dependent Anion Channel 1)
RN  - FYY3R43WGO (Minocycline)
RN  - Q1O6DSW23R (4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid)
RN  - 0 (Lipopolysaccharides)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Microglia/metabolism
MH  - *Sleep Deprivation/complications/metabolism
MH  - Voltage-Dependent Anion Channel 1/metabolism
MH  - Minocycline/pharmacology/metabolism
MH  - 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/metabolism
MH  - Lipopolysaccharides/metabolism
MH  - Pain, Postoperative
MH  - Inflammation/metabolism
MH  - Adenosine Triphosphate
OTO - NOTNLM
OT  - chronic postsurgical pain
OT  - microglia
OT  - skin/muscle incision and retraction (SMIR)
OT  - sleep deprivation
OT  - voltage-dependent anion channel 1
EDAT- 2023/02/25 06:00
MHDA- 2023/11/13 06:43
CRDT- 2023/02/24 12:22
PHST- 2022/09/15 00:00 [received]
PHST- 2023/01/30 00:00 [revised]
PHST- 2023/11/13 06:43 [medline]
PHST- 2023/02/25 06:00 [pubmed]
PHST- 2023/02/24 12:22 [entrez]
AID - 7056772 [pii]
AID - 10.1093/sleep/zsad039 [doi]
PST - ppublish
SO  - Sleep. 2023 Nov 8;46(11):zsad039. doi: 10.1093/sleep/zsad039.