PMID- 36829517
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230228
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 12
IP  - 2
DP  - 2023 Feb 3
TI  - Ageing-Induced Decline in Primary Myeloid Cell Phagocytosis Is Unaffected by 
      Optineurin Insufficiency.
LID - 10.3390/biology12020240 [doi]
LID - 240
AB  - Optineurin is a ubiquitin-binding adaptor protein involved in multiple cellular 
      processes, including innate inflammatory signalling. Mutations in optineurin were 
      found in amyotrophic lateral sclerosis, an adult-onset fatal neurodegenerative 
      disease that targets motor neurons. Neurodegeneration results in generation of 
      neuronal debris, which is primarily cleared by myeloid cells. To assess the role 
      of optineurin in phagocytosis, we performed a flow cytometry-based phagocytic 
      assay of apoptotic neuronal debris and E. coli bioparticles in bone 
      marrow-derived macrophages (BMDMs), and primary neonatal microglia from wild-type 
      (WT) and optineurin-insufficient (Optn(470T)) mice. We found no difference in 
      phagocytosis efficiency and the accompanying cytokine secretion in WT and 
      Optn(470T) BMDMs and microglia. This was true at both steady state and upon 
      proinflammatory polarization with lipopolysaccharide. When we analysed the effect 
      of ageing as a major risk factor for neurodegeneration, we found a substantial 
      decrease in the percentage of phagocytic cells and proinflammatory cytokine 
      secretion in BMDMs from 2-year-old mice. However, this ageing-induced phagocytic 
      decline was unaffected by optineurin insufficiency. All together, these results 
      indicate that ageing is the factor that perturbs normal phagocytosis and 
      proinflammatory cytokine secretion, but that optineurin is dispensable for these 
      processes.
FAU - Peradinovic, Josip
AU  - Peradinovic J
AD  - Laboratory for Molecular Immunology, Department of Biotechnology, University of 
      Rijeka, R. Matejcic 2, 51000 Rijeka, Croatia.
FAU - Mohovic, Nikolina
AU  - Mohovic N
AUID- ORCID: 0000-0001-7744-904X
AD  - Laboratory for Molecular Immunology, Department of Biotechnology, University of 
      Rijeka, R. Matejcic 2, 51000 Rijeka, Croatia.
FAU - Bulic, Katarina
AU  - Bulic K
AD  - Laboratory for Molecular Immunology, Department of Biotechnology, University of 
      Rijeka, R. Matejcic 2, 51000 Rijeka, Croatia.
FAU - Markovinovic, Andrea
AU  - Markovinovic A
AUID- ORCID: 0000-0003-1656-3150
AD  - Department of Basic and Clinical Neuroscience, Maurice Wohl Clinical Neuroscience 
      Institute, Institute of Psychiatry, Psychology and Neuroscience, King's College 
      London, London SE5 9RT, UK.
FAU - Cimbro, Raffaello
AU  - Cimbro R
AD  - Laboratory for Molecular Immunology, Department of Biotechnology, University of 
      Rijeka, R. Matejcic 2, 51000 Rijeka, Croatia.
FAU - Munitic, Ivana
AU  - Munitic I
AD  - Laboratory for Molecular Immunology, Department of Biotechnology, University of 
      Rijeka, R. Matejcic 2, 51000 Rijeka, Croatia.
LA  - eng
GR  - IP-2018-01-8563/Croatian Science Foundation/
GR  - 18-211-1369/University of Rijeka/
PT  - Journal Article
DEP - 20230203
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC9953198
OTO - NOTNLM
OT  - ageing
OT  - inflammation
OT  - neurodegeneration
OT  - optineurin
OT  - phagocytosis
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/02/26 06:01
PMCR- 2023/02/03
CRDT- 2023/02/25 01:03
PHST- 2022/12/23 00:00 [received]
PHST- 2023/01/31 00:00 [revised]
PHST- 2023/01/31 00:00 [accepted]
PHST- 2023/02/25 01:03 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/02/26 06:01 [medline]
PHST- 2023/02/03 00:00 [pmc-release]
AID - biology12020240 [pii]
AID - biology-12-00240 [pii]
AID - 10.3390/biology12020240 [doi]
PST - epublish
SO  - Biology (Basel). 2023 Feb 3;12(2):240. doi: 10.3390/biology12020240.