PMID- 36829580
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230228
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 12
IP  - 2
DP  - 2023 Feb 14
TI  - Levels of Circulating IgM and IgY Natural Antibodies in Broiler Chicks: 
      Association with Genotype and Farming Systems.
LID - 10.3390/biology12020304 [doi]
LID - 304
AB  - Naturally occurring antibodies (NAbs), which are major components of innate 
      immunity, exist in circulation under healthy conditions without prior antigenic 
      stimulation and are able to recognize both self- and non-self-constituents. The 
      present study aimed at identifying potential immunological differences between 
      commercial fast- and slow-growth broilers (n = 555) raised in conventional and 
      free-range systems, respectively, through the use of the specificity, isotypes 
      and levels of circulating NAbs. The possible beneficial effect of oregano-based 
      dietary supplementation was also evaluated. To this end, serum IgM and IgY NAbs 
      against self- (actin and DNA) and non-self- antigens (trinitrophenol and 
      lipopolysaccharide) were measured by ELISA and further correlated with genotype, 
      season and performance. Significantly higher levels of IgM NAbs against all 
      antigens were found in slow-growth compared to fast-growth broilers. IgM NAb 
      levels were also significantly increased in dietarily supplemented slow-growth 
      broilers versus those consuming standard feed. Moreover, significantly elevated 
      levels of anti-DNA IgY NAbs were found in fast-growth compared to slow-growth 
      broilers, whereas the opposite was observed for anti-LPS IgY NAbs. Multivariate 
      linear regression analysis confirmed multiple interactions between NAb levels, 
      genotype, season and performance. Overall, serum NAbs have proven to be valuable 
      innovative immunotools in the poultry industry, efficiently differentiating 
      fast-growing versus slow-growing broilers, and dietary supplementation of plant 
      extracts can enhance natural immunity.
FAU - Sarrigeorgiou, Ioannis
AU  - Sarrigeorgiou I
AD  - Immunology Laboratory, Immunology Department, Hellenic Pasteur Institute (HPI), 
      127, Vasilissis Sofias Avenue, 11521 Athens, Greece.
FAU - Stivarou, Theodora
AU  - Stivarou T
AUID- ORCID: 0000-0002-3743-844X
AD  - Immunology Laboratory, Immunology Department, Hellenic Pasteur Institute (HPI), 
      127, Vasilissis Sofias Avenue, 11521 Athens, Greece.
FAU - Tsinti, Gerasimina
AU  - Tsinti G
AUID- ORCID: 0000-0002-7162-4377
AD  - Immunology Laboratory, Immunology Department, Hellenic Pasteur Institute (HPI), 
      127, Vasilissis Sofias Avenue, 11521 Athens, Greece.
FAU - Patsias, Apostolos
AU  - Patsias A
AUID- ORCID: 0000-0003-2936-2083
AD  - Microbiology and Chemical Laboratory, Pindos APSI, 45500 Rodotopi Ioannina, 
      Greece.
FAU - Fotou, Evgenia
AU  - Fotou E
AUID- ORCID: 0000-0003-0368-0526
AD  - Department of Chemistry, Section of Organic Chemistry and Biochemistry, 
      University of Ioannina, 45110 Ioannina, Greece.
FAU - Moulasioti, Vasiliki
AU  - Moulasioti V
AUID- ORCID: 0000-0001-7075-1342
AD  - Department of Chemistry, Section of Organic Chemistry and Biochemistry, 
      University of Ioannina, 45110 Ioannina, Greece.
FAU - Kyriakou, Dimitra
AU  - Kyriakou D
AD  - Department of Chemistry, Section of Organic Chemistry and Biochemistry, 
      University of Ioannina, 45110 Ioannina, Greece.
FAU - Tellis, Constantinos
AU  - Tellis C
AD  - Department of Chemistry, Section of Organic Chemistry and Biochemistry, 
      University of Ioannina, 45110 Ioannina, Greece.
FAU - Papadami, Maria
AU  - Papadami M
AUID- ORCID: 0000-0002-2499-3890
AD  - Department of Chemistry, Section of Organic Chemistry and Biochemistry, 
      University of Ioannina, 45110 Ioannina, Greece.
FAU - Moussis, Vassilios
AU  - Moussis V
AD  - Department of Chemistry, Section of Organic Chemistry and Biochemistry, 
      University of Ioannina, 45110 Ioannina, Greece.
FAU - Tsiouris, Vasileios
AU  - Tsiouris V
AUID- ORCID: 0000-0003-2970-2463
AD  - Microbiology and Chemical Laboratory, Pindos APSI, 45500 Rodotopi Ioannina, 
      Greece.
AD  - Unit of Avian Medicine, Faculty of Veterinary Medicine, School of Health 
      Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
FAU - Tsikaris, Vassilios
AU  - Tsikaris V
AUID- ORCID: 0000-0002-2847-7340
AD  - Department of Chemistry, Section of Organic Chemistry and Biochemistry, 
      University of Ioannina, 45110 Ioannina, Greece.
FAU - Tsoukatos, Demokritos
AU  - Tsoukatos D
AD  - Department of Chemistry, Section of Organic Chemistry and Biochemistry, 
      University of Ioannina, 45110 Ioannina, Greece.
FAU - Lymberi, Peggy
AU  - Lymberi P
AD  - Immunology Laboratory, Immunology Department, Hellenic Pasteur Institute (HPI), 
      127, Vasilissis Sofias Avenue, 11521 Athens, Greece.
LA  - eng
PT  - Journal Article
DEP - 20230214
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC9952908
OTO - NOTNLM
OT  - IgM and IgY antibodies
OT  - alternative housing systems
OT  - broilers
OT  - diet supplements
OT  - domestic plant extracts
OT  - free range
OT  - innate immunity
OT  - natural antibodies
OT  - oregano oil
OT  - poultry industry
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2023/02/26 06:00
MHDA- 2023/02/26 06:01
PMCR- 2023/02/14
CRDT- 2023/02/25 01:04
PHST- 2023/01/04 00:00 [received]
PHST- 2023/02/03 00:00 [revised]
PHST- 2023/02/10 00:00 [accepted]
PHST- 2023/02/25 01:04 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/02/26 06:01 [medline]
PHST- 2023/02/14 00:00 [pmc-release]
AID - biology12020304 [pii]
AID - biology-12-00304 [pii]
AID - 10.3390/biology12020304 [doi]
PST - epublish
SO  - Biology (Basel). 2023 Feb 14;12(2):304. doi: 10.3390/biology12020304.