PMID- 36829863
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230228
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 12
IP  - 2
DP  - 2023 Jan 28
TI  - Overexpression of an Antioxidant Enzyme APX1 in cpr5 Mutant Restores its 
      Pleiotropic Growth Phenotype.
LID - 10.3390/antiox12020301 [doi]
LID - 301
AB  - Breeding crops with enhanced immunity is an effective strategy to reduce yield 
      loss caused by pathogens. The constitutive expresser of pathogenesis-related 
      genes (cpr5) mutant shows enhanced pathogen resistance but retarded growth; thus, 
      it restricts the application of cpr5 in breeding crops with disease resistance. 
      Reactive oxygen species (ROS) play important roles in plant growth and defense. 
      In this study, we determined that the cpr5 mutant exhibited excessive ROS 
      accumulation. However, the mutation of respiratory burst oxidase homolog D 
      (RBOHD), a reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 
      responsible for the production of ROS signaling in plant immunity, did not 
      suppress excessive ROS levels in cpr5. Furthermore, the cpr5 mutant showed low 
      levels of ascorbate peroxidase 1 (APX1), an important cytosolic ROS-scavenging 
      enzyme. APX1 overexpression in the cpr5 background removed excessive ROS and 
      restored the pleiotropic growth phenotype. Notably, APX1 overexpression did not 
      reduce the resistance of cpr5 mutant to virulent strain Pseudomonas syringae pv. 
      tomato (Pst) DC3000 and avirulent strain Pst DC3000 (avrRpt2). These results 
      suggest that the removal of excessive ROS by APX1 overexpression restored the 
      cpr5 growth phenotype while conserving pathogen resistance. Hence, our study 
      provides a theoretical and empirical basis for utilizing CPR5 in the breeding of 
      crops with disease resistance by effective oxidative stress management via APX1 
      expression.
FAU - Qi, Fan
AU  - Qi F
AUID- ORCID: 0000-0002-1963-1481
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-Products, Institute of Biotechnology, Zhejiang University, 
      Hangzhou 310058, China.
FAU - Li, Jianwei
AU  - Li J
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-Products, Institute of Biotechnology, Zhejiang University, 
      Hangzhou 310058, China.
FAU - Hong, Xiufang
AU  - Hong X
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-Products, Institute of Biotechnology, Zhejiang University, 
      Hangzhou 310058, China.
FAU - Jia, Zhiyi
AU  - Jia Z
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-Products, Institute of Biotechnology, Zhejiang University, 
      Hangzhou 310058, China.
FAU - Wu, Binyan
AU  - Wu B
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-Products, Institute of Biotechnology, Zhejiang University, 
      Hangzhou 310058, China.
FAU - Lin, Fucheng
AU  - Lin F
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-Products, Institute of Biotechnology, Zhejiang University, 
      Hangzhou 310058, China.
FAU - Liang, Yan
AU  - Liang Y
AD  - State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and 
      Safety of Agro-Products, Institute of Biotechnology, Zhejiang University, 
      Hangzhou 310058, China.
LA  - eng
GR  - 2021C02064-7, 2021C02009, and 2022C02016/Key Research and Development Program of 
      Zhejiang Province/
GR  - 31970279 and 32270289/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230128
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC9952838
OTO - NOTNLM
OT  - CPR5
OT  - ascorbate peroxidase
OT  - leaf senescence
OT  - reactive oxygen species
OT  - salicylic acid
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/02/26 06:01
PMCR- 2023/01/28
CRDT- 2023/02/25 01:08
PHST- 2022/12/06 00:00 [received]
PHST- 2023/01/22 00:00 [revised]
PHST- 2023/01/25 00:00 [accepted]
PHST- 2023/02/25 01:08 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/02/26 06:01 [medline]
PHST- 2023/01/28 00:00 [pmc-release]
AID - antiox12020301 [pii]
AID - antioxidants-12-00301 [pii]
AID - 10.3390/antiox12020301 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2023 Jan 28;12(2):301. doi: 10.3390/antiox12020301.