PMID- 36833223
OWN - NLM
STAT- MEDLINE
DCOM- 20230302
LR  - 20230306
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 14
IP  - 2
DP  - 2023 Jan 23
TI  - ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in 
      Taxane-Resistant MCF-7 Breast Cancer Sublines.
LID - 10.3390/genes14020296 [doi]
LID - 296
AB  - A limited number of studies are devoted to regulating TRIP6 expression in cancer. 
      Hence, we aimed to unveil the regulation of TRIP6 expression in MCF-7 breast 
      cancer cells (with high TRIP6 expression) and taxane-resistant MCF-7 sublines 
      (manifesting even higher TRIP6 expression). We found that TRIP6 transcription is 
      regulated primarily by the cyclic AMP response element (CRE) in hypomethylated 
      proximal promoters in both taxane-sensitive and taxane-resistant MCF-7 cells. 
      Furthermore, in taxane-resistant MCF-7 sublines, TRIP6 co-amplification with the 
      neighboring ABCB1 gene, as witnessed by fluorescence in situ hybridization 
      (FISH), led to TRIP6 overexpression. Ultimately, we found high TRIP6 mRNA levels 
      in progesterone receptor-positive breast cancer and samples resected from 
      premenopausal women.
FAU - Daniel, Petr
AU  - Daniel P
AUID- ORCID: 0000-0002-5523-4184
AD  - Department of Biochemistry, Cell and Molecular Biology, Third Faculty of 
      Medicine, Charles University, 100 00 Prague, Czech Republic.
FAU - Balusikova, Kamila
AU  - Balusikova K
AUID- ORCID: 0000-0002-0471-5877
AD  - Department of Biochemistry, Cell and Molecular Biology, Third Faculty of 
      Medicine, Charles University, 100 00 Prague, Czech Republic.
FAU - Vaclavikova, Radka
AU  - Vaclavikova R
AD  - Toxicogenomics Unit, National Institute of Public Health, 100 00 Prague, Czech 
      Republic.
AD  - Laboratory of Pharmacogenomics, Biomedical Center, Faculty of Medicine, Charles 
      University, 323 00 Pilsen, Czech Republic.
FAU - Seborova, Karolina
AU  - Seborova K
AUID- ORCID: 0000-0002-8925-2321
AD  - Toxicogenomics Unit, National Institute of Public Health, 100 00 Prague, Czech 
      Republic.
AD  - Laboratory of Pharmacogenomics, Biomedical Center, Faculty of Medicine, Charles 
      University, 323 00 Pilsen, Czech Republic.
FAU - Ransdorfova, Sarka
AU  - Ransdorfova S
AD  - Department of Cytogenetics, Institute of Hematology and Blood Transfusion, 128 00 
      Prague, Czech Republic.
FAU - Valerianova, Marie
AU  - Valerianova M
AD  - Department of Cytogenetics, Institute of Hematology and Blood Transfusion, 128 00 
      Prague, Czech Republic.
FAU - Wei, Longfei
AU  - Wei L
AD  - Department of Chemistry, Institute of Chemical Biology & Drug Discovery, Stony 
      Brook University-State University of New York, Stony Brook, NY 11794, USA.
FAU - Jelinek, Michael
AU  - Jelinek M
AD  - Department of Biochemistry, Cell and Molecular Biology, Third Faculty of 
      Medicine, Charles University, 100 00 Prague, Czech Republic.
FAU - Tlapakova, Tereza
AU  - Tlapakova T
AUID- ORCID: 0000-0001-8841-1573
AD  - Department of Cell Biology, Faculty of Science, Charles University, 128 00 
      Prague, Czech Republic.
FAU - Fleischer, Thomas
AU  - Fleischer T
AUID- ORCID: 0000-0003-0985-8460
AD  - Department of Cancer Genetics, Institute for Cancer Research, Oslo University 
      Hospital, 0310 Oslo, Norway.
FAU - Kristensen, Vessela N
AU  - Kristensen VN
AD  - Department of Medical Genetics, Institute of Clinical Medicine, Faculty of 
      Medicine, University of Oslo, 0424 Oslo, Norway.
FAU - Soucek, Pavel
AU  - Soucek P
AUID- ORCID: 0000-0002-4294-6799
AD  - Toxicogenomics Unit, National Institute of Public Health, 100 00 Prague, Czech 
      Republic.
AD  - Laboratory of Pharmacogenomics, Biomedical Center, Faculty of Medicine, Charles 
      University, 323 00 Pilsen, Czech Republic.
FAU - Ojima, Iwao
AU  - Ojima I
AUID- ORCID: 0000-0002-3628-1161
AD  - Department of Chemistry, Institute of Chemical Biology & Drug Discovery, Stony 
      Brook University-State University of New York, Stony Brook, NY 11794, USA.
FAU - Kovar, Jan
AU  - Kovar J
AUID- ORCID: 0000-0001-9432-8960
AD  - Department of Biochemistry, Cell and Molecular Biology, Third Faculty of 
      Medicine, Charles University, 100 00 Prague, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20230123
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (ABCB1 protein, human)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B)
RN  - E0399OZS9N (Cyclic AMP)
RN  - 0 (LIM Domain Proteins)
RN  - 1605-68-1 (taxane)
RN  - 0 (Taxoids)
RN  - 0 (Transcription Factors)
RN  - 0 (TRIP6 protein, human)
SB  - IM
MH  - Female
MH  - Humans
MH  - Adaptor Proteins, Signal Transducing/genetics
MH  - *ATP Binding Cassette Transporter, Subfamily B/genetics
MH  - Cyclic AMP
MH  - *Drug Resistance, Neoplasm/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *LIM Domain Proteins/genetics
MH  - MCF-7 Cells
MH  - *Neoplasms/genetics
MH  - Response Elements
MH  - Taxoids
MH  - Transcription Factors/genetics
PMC - PMC9957548
OTO - NOTNLM
OT  - ABCB1
OT  - CpG methylation
OT  - MCF-7
OT  - TRIP6
OT  - breast cancer
OT  - cAMP response element
OT  - gene amplification
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/01/23
CRDT- 2023/02/25 02:12
PHST- 2022/12/12 00:00 [received]
PHST- 2023/01/18 00:00 [revised]
PHST- 2023/01/19 00:00 [accepted]
PHST- 2023/02/25 02:12 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/01/23 00:00 [pmc-release]
AID - genes14020296 [pii]
AID - genes-14-00296 [pii]
AID - 10.3390/genes14020296 [doi]
PST - epublish
SO  - Genes (Basel). 2023 Jan 23;14(2):296. doi: 10.3390/genes14020296.