PMID- 36834841
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20230301
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 4
DP  - 2023 Feb 8
TI  - Neurovascular Unit Compensation from Adjacent Level May Contribute to Spontaneous 
      Functional Recovery in Experimental Cervical Spondylotic Myelopathy.
LID - 10.3390/ijms24043408 [doi]
LID - 3408
AB  - The progression and remission of cervical spondylotic myelopathy (CSM) are quite 
      unpredictable due to the ambiguous pathomechanisms. Spontaneous functional 
      recovery (SFR) has been commonly implicated in the natural course of incomplete 
      acute spinal cord injury (SCI), while the evidence and underlying pathomechanisms 
      of neurovascular unit (NVU) compensation involved in SFR remains poorly 
      understood in CSM. In this study, we investigate whether compensatory change of 
      NVU, in particular in the adjacent level of the compressive epicenter, is 
      involved in the natural course of SFR, using an established experimental CSM 
      model. Chronic compression was created by an expandable water-absorbing 
      polyurethane polymer at C5 level. Neurological function was dynamically assessed 
      by BBB scoring and somatosensory evoked potential (SEP) up to 2 months. 
      (Ultra)pathological features of NVUs were presented by histopathological and TEM 
      examination. Quantitative analysis of regional vascular profile area/number 
      (RVPA/RVPN) and neuroglial cells numbers were based on the specific EBA 
      immunoreactivity and neuroglial biomarkers, respectively. Functional integrity of 
      blood spinal cord barrier (BSCB) was detected by Evan blue extravasation test. 
      Although destruction of the NVU, including disruption of the BSCB, neuronal 
      degeneration and axon demyelination, as well as dramatic neuroglia reaction, were 
      found in the compressive epicenter and spontaneous locomotor and sensory function 
      recovery were verified in the modeling rats. In particular, restoration of BSCB 
      permeability and an evident increase in RVPA with wrapping proliferated 
      astrocytic endfeet in gray matter and neuron survival and synaptic plasticity 
      were confirmed in the adjacent level. TEM findings also proved ultrastructural 
      restoration of the NVU. Thus, NVU compensation changes in the adjacent level may 
      be one of the essential pathomechanisms of SFR in CSM, which could be a promising 
      endogenous target for neurorestoration.
FAU - Li, Guang-Sheng
AU  - Li GS
AD  - Spinal Division of Orthopedic and Traumatology Center, The Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524002, China.
AD  - Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong 
      Kong, China.
FAU - Chen, Guang-Hua
AU  - Chen GH
AUID- ORCID: 0000-0002-8877-1322
AD  - Spinal Division of Orthopedic and Traumatology Center, The Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524002, China.
FAU - Wang, Kang-Heng
AU  - Wang KH
AD  - Spinal Division of Orthopedic and Traumatology Center, The Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524002, China.
FAU - Wang, Xu-Xiang
AU  - Wang XX
AD  - Spinal Division of Orthopedic and Traumatology Center, The Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524002, China.
FAU - Hu, Xiao-Song
AU  - Hu XS
AD  - Spinal Division of Orthopedic and Traumatology Center, The Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524002, China.
FAU - Wei, Bo
AU  - Wei B
AD  - Spinal Division of Orthopedic and Traumatology Center, The Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524002, China.
FAU - Hu, Yong
AU  - Hu Y
AUID- ORCID: 0000-0003-0305-5616
AD  - Spinal Division of Orthopedic and Traumatology Center, The Affiliated Hospital of 
      Guangdong Medical University, Zhanjiang 524002, China.
AD  - Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong 
      Kong, China.
LA  - eng
GR  - 2021YFF0501604/National Key Research and Development Program of China/
GR  - 82072507/National Natural Science Foundation of China/
GR  - NO.2018A0303130105, NO.2016A030313679/Natural Science Foundation of Guangdong 
      Province, China/
PT  - Journal Article
DEP - 20230208
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Spinal Cord Compression/pathology
MH  - Recovery of Function
MH  - *Spondylosis/pathology
MH  - *Spinal Cord Diseases
MH  - Evoked Potentials, Somatosensory
MH  - *Spinal Cord Injuries
PMC - PMC9962900
OTO - NOTNLM
OT  - cervical spondylotic myelopathy
OT  - chronic compressive spinal cord injury
OT  - neurovascular unit compensation
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/08
CRDT- 2023/02/25 02:41
PHST- 2022/11/18 00:00 [received]
PHST- 2022/12/23 00:00 [revised]
PHST- 2023/02/06 00:00 [accepted]
PHST- 2023/02/25 02:41 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/08 00:00 [pmc-release]
AID - ijms24043408 [pii]
AID - ijms-24-03408 [pii]
AID - 10.3390/ijms24043408 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Feb 8;24(4):3408. doi: 10.3390/ijms24043408.