PMID- 36834930
OWN - NLM
STAT- MEDLINE
DCOM- 20230316
LR  - 20230316
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 4
DP  - 2023 Feb 9
TI  - 7,8-Dihydroxyflavone Attenuates Inflammatory Response and Insulin Resistance 
      Induced by the Paracrine Interaction between Adipocytes and Macrophages.
LID - 10.3390/ijms24043520 [doi]
LID - 3520
AB  - Obesity-induced inflammation and insulin resistance are mediated by macrophage 
      infiltration into adipose tissue. We investigated the effects of 
      7,8-dihydroxyflavone (7,8-DHF), a flavone found in plants, on the inflammatory 
      response and insulin resistance induced by the interaction between adipocytes and 
      macrophages. Hypertrophied 3T3-L1 adipocytes were cocultured with RAW 264.7 
      macrophages and treated with 7,8-DHF (3.12, 12.5, and 50 muM). The inflammatory 
      cytokines and free fatty acid (FFA) release were evaluated by assay kits, and 
      signaling pathways were determined by immunoblotting. Coculture of adipocytes and 
      macrophages increased inflammatory mediators, such as nitric oxide (NO), monocyte 
      chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-alpha), and 
      interleukin-6 (IL-6) and FFA secretion but suppressed the production of 
      anti-inflammatory adiponectin. 7,8-DHF counteracted the coculture-induced changes 
      (p < 0.001). 7,8-DHF also inhibited c-Jun N-terminal kinase (JNK) activation and 
      blocked nuclear factor kappa B (NF-kappaB) nuclear translocation in the coculture 
      system (p < 0.01). In addition, adipocytes cocultured with macrophages did not 
      increase glucose uptake and Akt phosphorylation in response to insulin. However, 
      7,8-DHF treatment recovered the impaired responsiveness to insulin (p < 0.01). 
      These findings show that 7,8-DHF alleviates inflammation and adipocyte 
      dysfunction in the coculture of hypertrophied 3T3-L1 adipocytes and RAW 264.7 
      macrophages, indicating its potential as a therapeutic agent for obesity-induced 
      insulin resistance.
FAU - Shin, Ye-Eun
AU  - Shin YE
AD  - Department of Food Science & Nutrition, The Catholic University of Korea, Bucheon 
      14662, Republic of Korea.
FAU - Choi, Ji Won
AU  - Choi JW
AD  - Department of Biotechnology, Graduate School, The Catholic University of Korea, 
      Bucheon 14662, Republic of Korea.
FAU - Park, Yong Il
AU  - Park YI
AD  - Department of Biotechnology, Graduate School, The Catholic University of Korea, 
      Bucheon 14662, Republic of Korea.
FAU - Kim, Hye-Kyeong
AU  - Kim HK
AUID- ORCID: 0000-0003-1659-1709
AD  - Department of Food Science & Nutrition, The Catholic University of Korea, Bucheon 
      14662, Republic of Korea.
LA  - eng
GR  - Research Fund,2022/The Catholic University of Korea/
PT  - Journal Article
DEP - 20230209
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (6,7-dihydroxyflavone)
RN  - 0 (Insulin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Flavones)
SB  - IM
MH  - Animals
MH  - Mice
MH  - 3T3-L1 Cells
MH  - *Adipocytes/metabolism
MH  - Coculture Techniques
MH  - *Inflammation/metabolism
MH  - Insulin/metabolism
MH  - *Insulin Resistance
MH  - *Macrophages/metabolism
MH  - Obesity/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - *Flavones/metabolism/pharmacology
MH  - Paracrine Communication
PMC - PMC9961847
OTO - NOTNLM
OT  - 7,8-dihydroxyflavone
OT  - adipocyte
OT  - inflammation
OT  - insulin resistance
OT  - macrophage
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/09
CRDT- 2023/02/25 02:42
PHST- 2023/01/02 00:00 [received]
PHST- 2023/02/07 00:00 [revised]
PHST- 2023/02/08 00:00 [accepted]
PHST- 2023/02/25 02:42 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/09 00:00 [pmc-release]
AID - ijms24043520 [pii]
AID - ijms-24-03520 [pii]
AID - 10.3390/ijms24043520 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Feb 9;24(4):3520. doi: 10.3390/ijms24043520.