PMID- 36835615
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20230301
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 4
DP  - 2023 Feb 20
TI  - GPR41 Regulates the Proliferation of BRECs via the PIK3-AKT-mTOR Pathway.
LID - 10.3390/ijms24044203 [doi]
LID - 4203
AB  - Short-chain fatty acids (SCFAs) play a pivotal role in regulating the 
      proliferation and development of bovine rumen epithelial cells (BRECs). G 
      protein-coupled receptor 41 (GPR41) is involved in the signal transduction in 
      BRECs as a receptor for SCFAs. Nevertheless, the impact of GPR41 on the 
      proliferation of BRECs has not been reported. The results of this research showed 
      that the knockdown of GPR41 (GRP41KD) decreased BRECs proliferation compared with 
      the wild-type BRECs (WT) (p < 0.001). The RNA sequencing (RNA-seq) analysis 
      showed that the gene expression profiles differed between WT and GPR41KD BRECs, 
      with the major differential genes enriched in phosphatidylinositol 3-kinase 
      (PIK3) signaling, cell cycle, and amino acid transport pathways (p < 0.05). The 
      transcriptome data were further validated by Western blot and qRT-PCR. It was 
      evident that the GPR41KD BRECs downregulated the level of the PIK3-Protein kinase 
      B (AKT)-mammalian target of the rapamycin (mTOR) signaling pathway core genes, 
      such as PIK3, AKT, eukaryotic translation initiation factor 4E binding protein 1 
      (4EBP1) and mTOR contrasted with the WT cells (p < 0.01). Furthermore, the 
      GPR41KD BRECs downregulated the level of Cyclin D2 p < 0.001) and Cyclin E2 (p < 
      0.05) compared with the WT cells. Therefore, it was proposed that GPR41 may 
      affect the proliferation of BRECs by mediating the PIK3-AKT-mTOR signaling 
      pathway.
FAU - Meng, Zitong
AU  - Meng Z
AUID- ORCID: 0000-0001-6913-1162
AD  - Institute of Animal Culture Collection and Application, College of Animal Science 
      and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Tan, Dejin
AU  - Tan D
AD  - Institute of Animal Culture Collection and Application, College of Animal Science 
      and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Cheng, Zhiqiang
AU  - Cheng Z
AUID- ORCID: 0000-0003-4911-6285
AD  - Institute of Animal Culture Collection and Application, College of Animal Science 
      and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Jiang, Maocheng
AU  - Jiang M
AUID- ORCID: 0000-0002-4533-101X
AD  - Institute of Animal Culture Collection and Application, College of Animal Science 
      and Technology, Yangzhou University, Yangzhou 225009, China.
FAU - Zhan, Kang
AU  - Zhan K
AD  - Institute of Animal Culture Collection and Application, College of Animal Science 
      and Technology, Yangzhou University, Yangzhou 225009, China.
AD  - Institutes of Agricultural Science and Technology Development, Yangzhou 
      University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
LA  - eng
PT  - Journal Article
DEP - 20230220
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Fatty Acids, Volatile)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Proliferation
MH  - Epithelial Cells/metabolism
MH  - Fatty Acids, Volatile/metabolism
MH  - *Phosphatidylinositol 3-Kinase/metabolism
MH  - *Proto-Oncogene Proteins c-akt/metabolism
MH  - Rumen
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC9963637
OTO - NOTNLM
OT  - BRECs
OT  - GPR41
OT  - PIK3-AKT-mTOR
OT  - proliferation
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/20
CRDT- 2023/02/25 02:56
PHST- 2022/12/02 00:00 [received]
PHST- 2023/02/06 00:00 [revised]
PHST- 2023/02/16 00:00 [accepted]
PHST- 2023/02/25 02:56 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/20 00:00 [pmc-release]
AID - ijms24044203 [pii]
AID - ijms-24-04203 [pii]
AID - 10.3390/ijms24044203 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Feb 20;24(4):4203. doi: 10.3390/ijms24044203.