PMID- 36835723
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230301
IS  - 2075-4450 (Print)
IS  - 2075-4450 (Electronic)
IS  - 2075-4450 (Linking)
VI  - 14
IP  - 2
DP  - 2023 Feb 3
TI  - Two Alimentary Canal Proteins, Fo-G(N) and Fo-Cyp1, Act in Western Flower Thrips, 
      Frankliniella occidentalis TSWV Infection.
LID - 10.3390/insects14020154 [doi]
LID - 154
AB  - Tomato spotted wilt virus (TSWV) is a plant virus that causes massive economic 
      damage to high-valued crops. This virus is transmitted by specific thrips, 
      including the western flower thrips, Frankliniella occidentalis. TSWV is acquired 
      by the young larvae during feeding on infected host plants. TSWV infects the gut 
      epithelium through hypothetical receptor(s) and multiplies within the cells for 
      subsequent horizontal transmission to other plant hosts via the salivary glands 
      during feeding. Two alimentary canal proteins, glycoprotein (Fo-G(N)) and 
      cyclophilin (Fo-Cyp1), are thought to be associated with the TSWV entry into the 
      gut epithelium of F. occidentalis. Fo-G(N) possesses a chitin-binding domain, and 
      its transcript was localized on the larval gut epithelium by fluorescence in situ 
      hybridization (FISH) analysis. Phylogenetic analysis indicated that F. 
      occidentalis encodes six cyclophilins, in which Fo-Cyp1 is closely related to a 
      human cyclophilin A, an immune modulator. The Fo-Cyp1 transcript was also 
      detected in the larval gut epithelium. Expression of these two genes was 
      suppressed by feeding their cognate RNA interference (RNAi) to young larvae. The 
      RNAi efficiencies were confirmed by the disappearance of the target gene 
      transcripts from the gut epithelium by FISH analyses. The RNAi treatments 
      directed to Fo-G(N) or Fo-Cyp1 prevented the typical TSWV titer increase after 
      the virus feeding, compared to control RNAi treatment. Our immunofluorescence 
      assay using a specific antibody to TSWV documented the reduction of TSWV in the 
      larval gut and adult salivary gland after the RNAi treatments. These results 
      support our hypothesis that the candidate proteins Fo-G(N) and Fo-Cyp1 act in 
      TSWV entry and multiplication in F. occidentalis.
FAU - Khan, Falguni
AU  - Khan F
AD  - Department of Plant Medicals, Andong National University, Andong 36729, Republic 
      of Korea.
FAU - Stanley, David
AU  - Stanley D
AD  - Biological Control of Insects Research Laboratory, USDA/ARS, 1503 S Providence 
      Road, Columbia, MO 65203, USA.
FAU - Kim, Yonggyun
AU  - Kim Y
AD  - Department of Plant Medicals, Andong National University, Andong 36729, Republic 
      of Korea.
LA  - eng
GR  - PJ01578901/Rural Development Administration/
PT  - Journal Article
DEP - 20230203
PL  - Switzerland
TA  - Insects
JT  - Insects
JID - 101574235
PMC - PMC9965231
OTO - NOTNLM
OT  - FISH
OT  - Frankliniella occidentalis
OT  - RNAi
OT  - TSWV
OT  - cyclophilin
OT  - glycoprotein
OT  - immunofluorescence assay
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/02/26 06:01
PMCR- 2023/02/03
CRDT- 2023/02/25 02:59
PHST- 2023/01/07 00:00 [received]
PHST- 2023/01/30 00:00 [revised]
PHST- 2023/02/01 00:00 [accepted]
PHST- 2023/02/25 02:59 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/02/26 06:01 [medline]
PHST- 2023/02/03 00:00 [pmc-release]
AID - insects14020154 [pii]
AID - insects-14-00154 [pii]
AID - 10.3390/insects14020154 [doi]
PST - epublish
SO  - Insects. 2023 Feb 3;14(2):154. doi: 10.3390/insects14020154.