PMID- 36836906
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230301
IS  - 2075-1729 (Print)
IS  - 2075-1729 (Electronic)
IS  - 2075-1729 (Linking)
VI  - 13
IP  - 2
DP  - 2023 Feb 16
TI  - Impact of Comorbidity of Bronchial Asthma and Type 2 Diabetes Mellitus on the 
      Expression and Functional Activity of TLR2 and TLR4 Receptors.
LID - 10.3390/life13020550 [doi]
LID - 550
AB  - Epidemiological data indicate the active progression of various forms of diabetes 
      mellitus in patients with bronchial asthma (BA), but little is known about the 
      mechanisms of comorbidity formation. TLR2 and TLR4 are involved in the 
      progression of asthma and type 2 diabetes mellitus (T2DM). These receptors are 
      involved in the inflammatory response to Gram(+) and Gram(-) bacteria, 
      respectively, so changes in their expression may affect the predisposition of 
      patients to bacteremia. The aim of this study was to analyze the expression and 
      functional activity of toll-like receptor 2 and 4 (TLR2 and TLR4) on peripheral 
      blood cells of patients with BA, T2DM, and BA + T2DM. The expression of TLR2 and 
      TLR4 was analyzed by flow cytometry. Whole blood samples were incubated with 
      lipopolysaccharides from E. coli (LPS) and lipoteichoic acid from S. pyogenes 
      (LTA). The concentration of cytokines and soluble blood proteins was determined 
      by ELISA. Patients with comorbid diseases showed a statistically significant 
      increase in TLR2 expression on both monocytes and neutrophils compared with 
      healthy donors and patients with BA. We found increased expression of TLR4 on the 
      surface of blood monocytes from patients compared to donors. The activation of 
      blood cells of patients and donors with LPS or LTA led to an increase in the 
      expression of "fast" pro-inflammatory cytokines (TNF-alpha, IL-6). In patients with 
      BA, the average production of TNF-alpha in response to endotoxin was two times higher 
      than in other studied groups. The reactions of blood cells in patients with T2DM 
      and BA + T2DM did not differ significantly. The expression and functional 
      activity of TLR2 and TLR4 on the blood cells of patients with comorbid disease 
      were similar to those only in patients with T2DM. The greatest increase in the 
      synthesis of the pro-inflammatory cytokine TNF-alpha in response to LPS and LTA was 
      observed in patients with BA, which can lead to an inadequate response to 
      bacteremia.
FAU - Radzyukevich, Yaroslav V
AU  - Radzyukevich YV
AUID- ORCID: 0000-0002-7483-2411
AD  - Hospital of Pushchino Scientific Center, Russian Academy of Sciences, Pushchino 
      142290, Russia.
AD  - Department of Molecular Biomedicine, Institute of Basic Biological Problems, 
      Federal Research Center "Pushchino Scientific Center for Biological Research of 
      the Russian Academy of Sciences", Pushchino 142290, Russia.
FAU - Kosyakova, Ninel I
AU  - Kosyakova NI
AD  - Hospital of Pushchino Scientific Center, Russian Academy of Sciences, Pushchino 
      142290, Russia.
FAU - Prokhorenko, Isabella R
AU  - Prokhorenko IR
AD  - Department of Molecular Biomedicine, Institute of Basic Biological Problems, 
      Federal Research Center "Pushchino Scientific Center for Biological Research of 
      the Russian Academy of Sciences", Pushchino 142290, Russia.
LA  - eng
GR  - capital A, Cyrilliccapital A, Cyrilliccapital A, Cyrilliccapital A, Cyrillic-capital A, Cyrillic20-120052090012-8/The Ministry of Education and Science of the Russian 
      Federation/
PT  - Journal Article
DEP - 20230216
PL  - Switzerland
TA  - Life (Basel)
JT  - Life (Basel, Switzerland)
JID - 101580444
PMC - PMC9965069
OTO - NOTNLM
OT  - bronchial asthma
OT  - comorbidity
OT  - lipopolysaccharides
OT  - lipoteichoic acid
OT  - toll-like receptors
OT  - type 2 diabetes mellitus
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/02/26 06:01
PMCR- 2023/02/16
CRDT- 2023/02/25 03:21
PHST- 2022/12/12 00:00 [received]
PHST- 2023/02/13 00:00 [revised]
PHST- 2023/02/14 00:00 [accepted]
PHST- 2023/02/25 03:21 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/02/26 06:01 [medline]
PHST- 2023/02/16 00:00 [pmc-release]
AID - life13020550 [pii]
AID - life-13-00550 [pii]
AID - 10.3390/life13020550 [doi]
PST - epublish
SO  - Life (Basel). 2023 Feb 16;13(2):550. doi: 10.3390/life13020550.