PMID- 36838575
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20230301
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 28
IP  - 4
DP  - 2023 Feb 7
TI  - Cross-Serological Reaction of Glandless Cottonseed Proteins to Peanut and Tree 
      Nut Allergic IgE.
LID - 10.3390/molecules28041587 [doi]
LID - 1587
AB  - Food allergy is a potentially life-threatening health concern caused by 
      immunoglobulin E (IgE) antibodies that mistakenly recognize normally harmless 
      food proteins as threats. Peanuts and tree nuts contain several seed storage 
      proteins that commonly act as allergens. Glandless cottonseed, lacking the toxic 
      compound gossypol, is a new food source. However, the seed storage proteins in 
      cottonseed may act as allergens. To assess this risk, glandless cottonseed 
      protein extracts were evaluated for IgE binding by peanut and tree nut allergic 
      volunteers. ELISA demonstrated that 25% of 32 samples had significant binding to 
      cottonseed extracts. Immunoblot analysis with pooled sera indicated that IgE 
      recognized a pair of bands migrating at approximately 50 kDa. Excision of these 
      bands and subsequent mass-spectrometric analysis demonstrated peptide matches to 
      cotton C72 and GC72 vicilin and legumin A and B proteins. Further, in silico 
      analysis indicated similarity of the cotton vicilin and legumin proteins to 
      peanut vicilin (Ara h 1) and cashew nut legumin (Ana o 2) IgE-binding epitopes 
      among others. The observations suggest both the cotton vicilin and legumin 
      proteins were recognized by the nut allergic IgE, and they should be considered 
      for future allergen risk assessments evaluating glandless cottonseed protein 
      products.
FAU - Mattison, Christopher P
AU  - Mattison CP
AUID- ORCID: 0000-0001-7819-0914
AD  - USDA-ARS, Southern Regional Research Center, New Orleans, LA 70124, USA.
FAU - He, Zhongqi
AU  - He Z
AUID- ORCID: 0000-0003-3507-5013
AD  - USDA-ARS, Southern Regional Research Center, New Orleans, LA 70124, USA.
FAU - Zhang, Dunhua
AU  - Zhang D
AD  - USDA-ARS, Aquatic Animal Health Research Unit, Auburn, AL 36832, USA.
FAU - Dupre, Rebecca
AU  - Dupre R
AD  - USDA-ARS, Southern Regional Research Center, New Orleans, LA 70124, USA.
AD  - Oak Ridge Institute for Science and Education, U.S. Department of Energy, Oak 
      Ridge, TN 37831, USA.
FAU - Lloyd, Steven W
AU  - Lloyd SW
AUID- ORCID: 0000-0002-8876-7913
AD  - USDA-ARS, Southern Regional Research Center, New Orleans, LA 70124, USA.
LA  - eng
GR  - 6054-43440-046-00-D and 6054-41000-113-000-D/United States Department of 
      Agriculture/
PT  - Journal Article
DEP - 20230207
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Cottonseed Oil)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Allergens)
RN  - 0 (Seed Storage Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Antigens, Plant)
SB  - IM
MH  - Humans
MH  - Nuts
MH  - Arachis/metabolism
MH  - Cottonseed Oil
MH  - Immunoglobulin E
MH  - Allergens/chemistry
MH  - *Food Hypersensitivity
MH  - *Fabaceae/metabolism
MH  - Seed Storage Proteins
MH  - Plant Proteins/metabolism
MH  - Antigens, Plant
PMC - PMC9967559
OTO - NOTNLM
OT  - cottonseed
OT  - cross-reaction
OT  - food allergy
OT  - glandless
OT  - immunoglobulin E (IgE)
OT  - legumin
OT  - peanut
OT  - tree nut
OT  - vicilin
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/07
CRDT- 2023/02/25 03:54
PHST- 2022/11/18 00:00 [received]
PHST- 2023/01/18 00:00 [revised]
PHST- 2023/02/03 00:00 [accepted]
PHST- 2023/02/25 03:54 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/07 00:00 [pmc-release]
AID - molecules28041587 [pii]
AID - molecules-28-01587 [pii]
AID - 10.3390/molecules28041587 [doi]
PST - epublish
SO  - Molecules. 2023 Feb 7;28(4):1587. doi: 10.3390/molecules28041587.