PMID- 36838879
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20230301
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 28
IP  - 4
DP  - 2023 Feb 16
TI  - Ultrarapid Microwave-Assisted Synthesis of Fluorescent Silver Coordination 
      Polymer Nanoparticles and Its Application in Detecting Alkaline Phosphatase 
      Activity.
LID - 10.3390/molecules28041892 [doi]
LID - 1892
AB  - Fluorescent silver coordination polymer nanoparticles (Ag-TPA CPNs) were 
      synthesized using a combination of terephthalic acid (TPA) and silver nitrate via 
      an ultrarapid microwave-assisted strategy within 15 min. The Ag-TPA CPNs 
      displayed a high fluorescent quantum yield (QY = 20.19%) and large Stokes shift 
      (~200 nm), with two emission peaks at 490 nm and 520 nm under an excitation 
      wavelength of 320 nm. A fluorescent "turn-off" method using fluorescent Ag-TPA 
      CPNs was applied to detect the alkaline phosphatase (ALP) activity on the basis 
      of the ALP-catalyzed hydrolysis of ascorbic acid 2-phosphate (AA2P) to ascorbic 
      acid (AA), and the AA product triggered the reduction of Ag(+) ions into silver 
      nanoparticles. The fluorescent lifetime of Ag-TPA CPNs decreased from 3.93 ms to 
      3.80 ms after the addition of ALP, which suggests that this fluorescent 
      "turn-off" detection of ALP activity is a dynamic quenching process. The 
      fluorescent intensity had a linear relationship with the concentration of ALP in 
      the range of 0.2-12 mU/mL (r = 0.991) and with a limit of detection (LOD) of 0.07 
      mU/mL. It showed high selectivity in ALP detection towards metal ions and amino 
      acids, as well as other enzymes such as horseradish peroxidase, glucose oxidase, 
      tyrosinase, trypsin, lysozyme, and superoxides. When it was applied for the 
      fluorescent "turn-off" detection of ALP activity in serum samples, mean recovery 
      levels ranging from 99.5% to 101.2% were obtained, with relative standard 
      deviations (RSDs) lower than 4% accuracy. Therefore, it is an efficient and 
      accurate tool for analyzing ALP levels in biosamples.
FAU - Pei, Kanglin
AU  - Pei K
AD  - Chongqing Key Laboratory of Green Synthesis and Applications, College of 
      Chemistry, Chongqing Normal University, Chongqing 401331, China.
FAU - Li, Di
AU  - Li D
AD  - Department of Pharmacy, the Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing 401331, China.
FAU - Qi, Wenjing
AU  - Qi W
AUID- ORCID: 0000-0001-9371-3154
AD  - Chongqing Key Laboratory of Green Synthesis and Applications, College of 
      Chemistry, Chongqing Normal University, Chongqing 401331, China.
FAU - Wu, Di
AU  - Wu D
AD  - Chongqing Key Laboratory of Green Synthesis and Applications, College of 
      Chemistry, Chongqing Normal University, Chongqing 401331, China.
LA  - eng
GR  - 02030307-00042/Top-Notch Young Innovative Talents of Chongqing Normal University/
GR  - KJQN201900521/Scientific and Technological Research Program of Chongqing 
      Education Committee/
GR  - cstc2021ycjh-bgzxm0131/Innovation and Entrepreneurship Team of Inorganic 
      Optoelectronic Functional Materials for Chongqing Yingcai/
PT  - Journal Article
DEP - 20230216
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - 0 (Polymers)
RN  - 3M4G523W1G (Silver)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - *Alkaline Phosphatase/metabolism
MH  - *Metal Nanoparticles/chemistry
MH  - Polymers
MH  - Microwaves
MH  - Silver
MH  - Fluorescent Dyes/chemistry
MH  - Limit of Detection
PMC - PMC9965027
OTO - NOTNLM
OT  - alkaline phosphatase
OT  - ascorbic acid
OT  - ascorbic acid 2-phosphate
OT  - fluorescent silver coordination polymer nanoparticles
OT  - microwave-assisted synthesis
COIS- The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/16
CRDT- 2023/02/25 04:00
PHST- 2023/01/09 00:00 [received]
PHST- 2023/02/07 00:00 [revised]
PHST- 2023/02/15 00:00 [accepted]
PHST- 2023/02/25 04:00 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/16 00:00 [pmc-release]
AID - molecules28041892 [pii]
AID - molecules-28-01892 [pii]
AID - 10.3390/molecules28041892 [doi]
PST - epublish
SO  - Molecules. 2023 Feb 16;28(4):1892. doi: 10.3390/molecules28041892.