PMID- 36839409
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20230301
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 15
IP  - 4
DP  - 2023 Feb 20
TI  - Multi-Omics Analysis Reveals the Potential Effects of Maternal Dietary 
      Restriction on Fetal Muscle Growth and Development.
LID - 10.3390/nu15041051 [doi]
LID - 1051
AB  - In terms of fetal muscle growth, development, and health, maternal nutrition is a 
      crucial influence, although the exact biochemical mechanism by which this occurs 
      is still not fully understood. To examine the potential impacts of maternal 
      dietary restriction on fetal muscle development, the sheep maternal dietary 
      restriction model was developed for this study. In our study, 12 pregnant ewes 
      were evenly split into two experimental groups and fed either 75% or 100% of a 
      maternal nutrient. In addition, a multi-omics analysis was used to study the 
      embryonic longissimus dorsis on gestational days (GD) 85 and 135. The fetal 
      weight at GD 135 was significantly below normal due to the maternal restricted 
      diet (p < 0.01). When fetuses were exposed to the dietary deficit, 416 mRNAs and 
      40 proteins were significantly changed. At GD 85, the multi-omics analysis 
      revealed that maternal dietary restriction led to a significant up-regulation of 
      the cell cycle regulator CDK2 gene in the cellular senescence signaling pathway, 
      and the results of the qRT-PCR were similar to the multi-omics analysis, which 
      showed that SIX1, PAX7, the cell cycle factors CDK4 and CDK6, and the BCL-2 
      apoptosis factor were up-regulated and several skeletal muscle marker genes, such 
      as MYF5 and MyoD were down-regulated. At GD 135, maternal dietary restriction 
      blocks the muscle fiber differentiation and maturation. The multi-omics analysis 
      revealed that the TEAD1 gene was in the Hippo signaling pathway, the muscle 
      marker genes MYF5 and MyoG were significantly down-regulated, and the TEAD1 
      binding of the down-regulated VGLL3 gene might be potential mechanisms affecting 
      myofiber differentiation and maturation. Knocking down the CDK2 gene could 
      inhibit the proliferation of primary embryonic myoblasts, and the expression 
      levels of cell cycle regulatory factors CDK4 and CDK6 were significantly changed. 
      Under low nutrient culture conditions, the number of myoblasts decreased and the 
      expression of CDK2, CDK6, MYF5, PAX7 and BCL-2 changed, which was in perfect 
      agreement with the multi-omics analysis. All of the findings from our study 
      helped to clarify the potential effects of maternal dietary restriction on fetal 
      muscle growth and development. They also provided a molecular foundation for 
      understanding the molecular regulatory mechanisms of maternal nutrition on fetal 
      muscle growth and development, as well as for the development of new medications 
      and the management of related metabolic diseases.
FAU - Wang, Xinyue
AU  - Wang X
AUID- ORCID: 0000-0002-8025-1760
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Shang, Mingyu
AU  - Shang M
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Hu, Wenping
AU  - Hu W
AUID- ORCID: 0000-0002-8962-7541
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Zhang, Li
AU  - Zhang L
AUID- ORCID: 0000-0002-1197-843X
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
LA  - eng
GR  - 2020ZD0003/Inner Mongolia Sciences & Technology Plan/
GR  - 2021YFD1300902/national natural science fund joint fund key project: National Key 
      R&D Program of China/
GR  - 2018-YF-06 and 2018-YF-09/University-Enterprise Cooperative R&D Project 
      "Research, Development and Application of Genome-Wide Selective Breeding 
      Technology for Hu Sheep"/
PT  - Journal Article
DEP - 20230220
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Pregnancy
MH  - Animals
MH  - Female
MH  - Sheep
MH  - *Multiomics
MH  - *Fetal Development
MH  - Diet/veterinary
MH  - Muscle, Skeletal
MH  - Proto-Oncogene Proteins c-bcl-2
MH  - Muscle Development
PMC - PMC9964303
OTO - NOTNLM
OT  - fetal muscle
OT  - maternal dietary restriction
OT  - multi-omics analysis
OT  - proteome
OT  - sheep pregnancy model
OT  - transcriptome
COIS- The authors have no competing interests.
EDAT- 2023/02/26 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/20
CRDT- 2023/02/25 04:10
PHST- 2023/01/30 00:00 [received]
PHST- 2023/02/15 00:00 [revised]
PHST- 2023/02/16 00:00 [accepted]
PHST- 2023/02/25 04:10 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/20 00:00 [pmc-release]
AID - nu15041051 [pii]
AID - nutrients-15-01051 [pii]
AID - 10.3390/nu15041051 [doi]
PST - epublish
SO  - Nutrients. 2023 Feb 20;15(4):1051. doi: 10.3390/nu15041051.