PMID- 36840468
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20240130
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 32
DP  - 2023 Jan-Dec
TI  - In Situ-Formed Fibrin Hydrogel Scaffold Loaded With Human Umbilical Cord 
      Mesenchymal Stem Cells Promotes Skin Wound Healing.
PG  - 9636897231156215
LID - 10.1177/09636897231156215 [doi]
LID - 09636897231156215
AB  - Healing of full-thickness skin wounds remains a major challenge. Recently, human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) were shown to possess an 
      extraordinary potential to promote skin repair in clinical settings. However, 
      their low survival rate after transplantation limits their therapeutic efficiency 
      in treating full-thickness skin wounds. Hydrogels are considered an ideal cell 
      transplantation vector owing to their three-dimensional mesh structure, good 
      biosafety, and biodegradation. The objective of this study was to investigate the 
      skin wound healing effect of a fibrin hydrogel scaffold loaded with hUC-MSCs. We 
      found that the fibrin hydrogel had a three-dimensional mesh structure and low 
      cytotoxicity and could prolong the time of cell survival in the peri-wound area. 
      The number of green fluorescent protein (GFP)-labeled hUC-MSCs was higher in the 
      full-thickness skin wound of mice treated with hydrogel-hUC-MSCs than those of 
      mice treated with cell monotherapy. In addition, the combination therapy between 
      the hydrogel and hUC-MSCs speed up wound closure, its wound healing rate was 
      significantly higher than those of phosphate-buffered saline (PBS) therapy, 
      hydrogel monotherapy, and hUC-MSCs monotherapy. Furthermore, the results showed 
      that the combination therapy between hydrogel and hUC-MSCs increased keratin 10 
      and keratin 14 immunofluorescence staining, and upregulated the relative gene 
      expressions of epidermal growth factor (EGF), transforming growth factor-beta1 
      (TGF-beta1), and vascular endothelial growth factor A (VEGFA), promoting epithelial 
      regeneration and angiogenesis. In conclusion, the fibrin hydrogel scaffold 
      provides a relatively stable sterile environment for cell adhesion, 
      proliferation, and migration, and prolongs cell survival at the wound site. The 
      hydrogel-hUC-MSCs combination therapy promotes wound closure, 
      re-epithelialization, and neovascularization. It exhibits a remarkable 
      therapeutic effect, being more effective than the monotherapy with hUC-MSCs or 
      hydrogel.
FAU - Hu, Lvzhong
AU  - Hu L
AUID- ORCID: 0000-0002-2760-8871
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow 
      University, Suzhou, China.
FAU - Zhou, Jinhua
AU  - Zhou J
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow 
      University, Suzhou, China.
FAU - He, Zhisong
AU  - He Z
AD  - Department of Cardiovascular Medicine, The First Affiliated Hospital of Soochow 
      University, Suzhou, China.
FAU - Zhang, Lin
AU  - Zhang L
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow 
      University, Suzhou, China.
FAU - Du, Fangzhou
AU  - Du F
AD  - Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of 
      Sciences, Suzhou, China.
FAU - Nie, Mengting
AU  - Nie M
AD  - Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of 
      Sciences, Suzhou, China.
AD  - School of Life Science and Technology, Changchun University of Science and 
      Technology, Changchun, China.
FAU - Zhou, Yao
AU  - Zhou Y
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow 
      University, Suzhou, China.
FAU - Hao, Hang
AU  - Hao H
AD  - Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of 
      Sciences, Suzhou, China.
FAU - Zhang, Lixing
AU  - Zhang L
AD  - Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of 
      Sciences, Suzhou, China.
FAU - Yu, Shuang
AU  - Yu S
AD  - Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of 
      Sciences, Suzhou, China.
AD  - Zhengzhou Institute of Engineering and Technology Affiliated to SIBET, Zhengzhou, 
      China.
AD  - Xuzhou Medical University, Xuzhou, China.
FAU - Zhang, Jingzhong
AU  - Zhang J
AD  - Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of 
      Sciences, Suzhou, China.
AD  - Zhengzhou Institute of Engineering and Technology Affiliated to SIBET, Zhengzhou, 
      China.
AD  - Xuzhou Medical University, Xuzhou, China.
FAU - Chen, Youguo
AU  - Chen Y
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow 
      University, Suzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Hydrogels)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Mice
MH  - Hydrogels
MH  - *Mesenchymal Stem Cell Transplantation/methods
MH  - *Mesenchymal Stem Cells
MH  - Umbilical Cord/cytology
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - *Wound Healing
MH  - Tissue Scaffolds
PMC - PMC9969468
OTO - NOTNLM
OT  - fibrin
OT  - human umbilical cord mesenchymal stem cells
OT  - hydrogel
OT  - skin wound healing
COIS- The author(s) declared no potential conflicts of interest with respect to the 
      research, authorship, and/or publication of this article.
EDAT- 2023/02/26 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/25
CRDT- 2023/02/25 04:53
PHST- 2023/02/25 04:53 [entrez]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/25 00:00 [pmc-release]
AID - 10.1177_09636897231156215 [pii]
AID - 10.1177/09636897231156215 [doi]
PST - ppublish
SO  - Cell Transplant. 2023 Jan-Dec;32:9636897231156215. doi: 
      10.1177/09636897231156215.