PMID- 36841398
OWN - NLM
STAT- MEDLINE
DCOM- 20230404
LR  - 20230505
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 110
DP  - 2023 Jun
TI  - Genetic variations of Plasmodium falciparum circumsporozoite protein and the 
      impact on interactions with human immunoproteins and malaria vaccine efficacy.
PG  - 105418
LID - S1567-1348(23)00016-3 [pii]
LID - 10.1016/j.meegid.2023.105418 [doi]
AB  - In October 2021, the world's first malaria vaccine RTS,S was endorsed by WHO for 
      broad use in children, despite its low efficacy. This study examined polyclonal 
      infections and the associations of parasite genetic variations with binding 
      affinity to human leukocyte antigen (HLA). Multiplicity of infection was 
      determined by amplicon deep sequencing of PfMSP1. Genetic variations in PfCSP 
      were examined across 88 samples from Ghana and analyzed together with 1655 PfCSP 
      sequences from other African and non-African isolates. Binding interactions of 
      PfCSP peptide variants and HLA were predicted using NetChop and HADDOCK. High 
      polyclonality was detected among infections, with each infection harboring 
      multiple non-3D7 PfCSP variants. Twenty-seven PfCSP haplotypes were detected in 
      the Ghanaian samples, and they broadly represented PfCSP diversity across Africa. 
      The number of genetic differences between 3D7 and non-3D7 PfCSP variants does not 
      influence binding to HLA. However, CSP peptide length after proteolytic 
      degradation significantly affects its molecular weight and binding affinity to 
      HLA. Despite the high diversity of HLA, the majority of the HLAI and II alleles 
      interacted/bound with all Ghana CSP peptides. Multiple non-3D7 strains among P. 
      falciparum infections could impact the effectiveness of RTS,S. Longer peptides of 
      the Th2R/Th3R CSP regions should be considered in future versions of RTS,S.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Dieng, Cheikh Cambel
AU  - Dieng CC
AD  - Department of Biological Sciences, University of North Carolina at Charlotte, 
      Charlotte, NC, USA. Electronic address: cdieng@uncc.edu.
FAU - Ford, Colby T
AU  - Ford CT
AD  - Department of Bioinformatics and Genomics, University of North Carolina at 
      Charlotte, Charlotte, NC, USA; School of Data Science, University of North 
      Carolina at Charlotte, Charlotte, NC, USA. Electronic address: 
      colby.ford@uncc.edu.
FAU - Lerch, Anita
AU  - Lerch A
AD  - Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA.
FAU - Doniou, Dickson
AU  - Doniou D
AD  - Department of Immunology, Noguchi Memorial Institute for Medical Research, 
      University of Ghana, Accra, Ghana.
FAU - Vegesna, Kovidh
AU  - Vegesna K
AD  - Department of Bioinformatics and Genomics, University of North Carolina at 
      Charlotte, Charlotte, NC, USA.
FAU - Janies, Daniel
AU  - Janies D
AD  - Department of Bioinformatics and Genomics, University of North Carolina at 
      Charlotte, Charlotte, NC, USA.
FAU - Cui, Liwang
AU  - Cui L
AD  - Department of Internal Medicine, Morsani College of Medicine, University of South 
      Florida, Tampa, FL, USA.
FAU - Amoah, Linda
AU  - Amoah L
AD  - Department of Immunology, Noguchi Memorial Institute for Medical Research, 
      University of Ghana, Accra, Ghana; West Africa Center for Cell Biology of 
      Infectious Pathogens, University of Ghana, Accra, Ghana.
FAU - Afrane, Yaw
AU  - Afrane Y
AD  - Department of Microbiology, University of Ghana Medical School, University of 
      Ghana, Accra, Ghana.
FAU - Lo, Eugenia
AU  - Lo E
AD  - Department of Biological Sciences, University of North Carolina at Charlotte, 
      Charlotte, NC, USA; School of Data Science, University of North Carolina at 
      Charlotte, Charlotte, NC, USA. Electronic address: eugenia.lo@uncc.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230224
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (Malaria Vaccines)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Immunoproteins)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Child
MH  - Humans
MH  - *Malaria Vaccines/genetics
MH  - Plasmodium falciparum
MH  - Ghana/epidemiology
MH  - Vaccine Efficacy
MH  - *Malaria
MH  - *Malaria, Falciparum/epidemiology/prevention & control
MH  - Protozoan Proteins
MH  - Immunoproteins/genetics/metabolism
MH  - Histocompatibility Antigens Class II/genetics
MH  - Genetic Variation
OTO - NOTNLM
OT  - Binding interactions
OT  - Circumsporozoite protein
OT  - Malaria vaccine
OT  - Multiplicity of infection
OT  - Plasmodium falciparum
OT  - RTS,S-induced immunity
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2023/02/26 06:00
MHDA- 2023/04/04 06:42
CRDT- 2023/02/25 19:30
PHST- 2023/01/03 00:00 [received]
PHST- 2023/02/17 00:00 [revised]
PHST- 2023/02/20 00:00 [accepted]
PHST- 2023/04/04 06:42 [medline]
PHST- 2023/02/26 06:00 [pubmed]
PHST- 2023/02/25 19:30 [entrez]
AID - S1567-1348(23)00016-3 [pii]
AID - 10.1016/j.meegid.2023.105418 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2023 Jun;110:105418. doi: 10.1016/j.meegid.2023.105418. Epub 
      2023 Feb 24.