PMID- 36844364 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230228 IS - 2452-199X (Electronic) IS - 2452-199X (Linking) VI - 25 DP - 2023 Jul TI - A "T.E.S.T." hydrogel bioadhesive assisted by corneal cross-linking for in situ sutureless corneal repair. PG - 333-346 LID - 10.1016/j.bioactmat.2023.02.006 [doi] AB - Corneal transplantation is an effective clinical treatment for corneal diseases, which, however, is limited by donor corneas. It is of great clinical value to develop bioadhesive corneal patches with functions of "Transparency" and "Epithelium & Stroma generation", as well as "Suturelessness" and "Toughness". To simultaneously meet the "T.E.S.T." requirements, a light-curable hydrogel is designed based on methacryloylated gelatin (GelMA), Pluronic F127 diacrylate (F127DA) & Aldehyded Pluronic F127 (AF127) co-assembled bi-functional micelles and collagen type I (COL I), combined with clinically applied corneal cross-linking (CXL) technology for repairing damaged cornea. The patch formed after 5 min of ultraviolet irradiation possesses transparent, highly tough, and strongly bio-adhesive performance. Multiple cross-linking makes the patch withstand deformation near 600% and exhibit a burst pressure larger than 400 mmHg, significantly higher than normal intraocular pressure (10-21 mmHg). Besides, the slower degradation than GelMA-F127DA&AF127 hydrogel without COL I makes hydrogel patch stable on stromal beds in vivo, supporting the regrowth of corneal epithelium and stroma. The hydrogel patch can replace deep corneal stromal defects and well bio-integrate into the corneal tissue in rabbit models within 4 weeks, showing great potential in surgeries for keratoconus and other corneal diseases by combining with CXL. CI - (c) 2023 The Authors. FAU - Li, Meiyan AU - Li M AD - Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China. AD - NHC Key Laboratory of Myopia (Fudan University), Shanghai, China. AD - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. FAU - Wei, Ruoyan AU - Wei R AD - Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China. AD - NHC Key Laboratory of Myopia (Fudan University), Shanghai, China. AD - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. FAU - Liu, Chang AU - Liu C AD - Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China. AD - NHC Key Laboratory of Myopia (Fudan University), Shanghai, China. AD - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. FAU - Fang, Haowei AU - Fang H AD - Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, Shanghai, China. FAU - Yang, Weiming AU - Yang W AD - Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China. AD - NHC Key Laboratory of Myopia (Fudan University), Shanghai, China. AD - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. AD - Department of Ophthalmology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China. FAU - Wang, Yunzhe AU - Wang Y AD - Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China. AD - NHC Key Laboratory of Myopia (Fudan University), Shanghai, China. AD - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. FAU - Xian, Yiyong AU - Xian Y AD - Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China. AD - NHC Key Laboratory of Myopia (Fudan University), Shanghai, China. AD - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. FAU - Zhang, Kunxi AU - Zhang K AD - Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, Shanghai, China. FAU - He, Yong AU - He Y AD - State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou, China. FAU - Zhou, Xingtao AU - Zhou X AD - Department of Ophthalmology, EYE & ENT Hospital of Fudan University Shanghai, China. AD - NHC Key Laboratory of Myopia (Fudan University), Shanghai, China. AD - Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China. LA - eng PT - Journal Article DEP - 20230211 PL - China TA - Bioact Mater JT - Bioactive materials JID - 101685294 PMC - PMC9946819 OTO - NOTNLM OT - AF127, Aldehyded Pluronic F127 OT - AS-OCT, Anterior Segment Optical Coherence Tomography OT - Bioadhesives OT - CCK-8, Cell Counting Kit-8 OT - COL I, Collagen Type I OT - CXL OT - CXL, Corneal Cross-linking OT - Corneal patch OT - DLS, Dynamic Light Scattering OT - DMEM, Dulbecco's Modified Eagle's Medium OT - ECM, Extracellular Matrix OT - F127DA, Pluronic F127 diacrylate OT - FBS, Fetal Bovine Serum OT - GelMA, Methacryloylated Gelatin OT - H&E, Hematoxylin and Eosin OT - IHC, Immunohistochemistry OT - IOP, Intraocular Pressure OT - PBS, Phosphate-buffered Saline OT - RF, Ribo fl avin-5-phosphate OT - ROS, Reactive Oxygen Species OT - SD, Standard Deviation OT - Sutureless repair OT - TEM, Transmission Electron Microscopy OT - Tough hydrogel OT - UV, Ultraviolet OT - alpha-SMA, Alpha Smooth Muscle Actin COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to in fl uence the work reported in this paper. EDAT- 2023/02/28 06:00 MHDA- 2023/02/28 06:01 PMCR- 2023/02/11 CRDT- 2023/02/27 04:17 PHST- 2022/10/23 00:00 [received] PHST- 2023/01/16 00:00 [revised] PHST- 2023/02/07 00:00 [accepted] PHST- 2023/02/27 04:17 [entrez] PHST- 2023/02/28 06:00 [pubmed] PHST- 2023/02/28 06:01 [medline] PHST- 2023/02/11 00:00 [pmc-release] AID - S2452-199X(23)00044-0 [pii] AID - 10.1016/j.bioactmat.2023.02.006 [doi] PST - epublish SO - Bioact Mater. 2023 Feb 11;25:333-346. doi: 10.1016/j.bioactmat.2023.02.006. eCollection 2023 Jul.