PMID- 36844639
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230228
IS  - 1319-562X (Print)
IS  - 2213-7106 (Electronic)
IS  - 2213-7106 (Linking)
VI  - 30
IP  - 3
DP  - 2023 Mar
TI  - The impact of SELP gene Thr715Pro polymorphism on sP-selectin level and 
      association with cardiovascular disease in Saudi diabetic patients: A 
      cross-sectional case-control study.
PG  - 103579
LID - 10.1016/j.sjbs.2023.103579 [doi]
LID - 103579
AB  - BACKGROUND: Cardiovascular diseases (CVD) are leading cause of mortality in 
      patients with type 2 diabetes mellitus (T2DM). Increased soluble sP-selectin and 
      715Thr > Pro polymorphism were studied in CVD and T2DM, but association between 
      them hasn't been explored in Saudi Arabia. We aimed to assess sP-selectin levels 
      in T2DM and T2DM-associated CVD patients in comparison to healthy control cohort. 
      Also, we sought to investigate relationship between Thr715Pro polymorphism and 
      sP-selectin levels and disease state. METHODS: This is a cross-sectional 
      case-control study. sP-selectin level (measured by Enzyme-linked immunosorbent 
      assay) and prevalence of Thr715Pro polymorphism (assessed by Sanger sequencing) 
      were investigated in 136 Saudi participants. The study comprised 3 groups: group1 
      included 41 T2DM patients; group 2 (48 T2DM patients with CVD), and group 3 (47 
      healthy controls). RESULTS: sP-selectin levels were significantly higher in 
      diabetics and diabetics + CVD groups as compared to the corresponding control. In 
      addition, results showed that the prevalence of 715Thr > Pro polymorphism is 
      11.75 % in the study population amongst the three study groups (9.55 % Thr/Pro, 
      and 2.2 % Pro/Pro). No statistical difference was found between sP-selectin 
      levels in subject carrying the wildtype genotype of this polymorphism and these 
      who carry the mutant gene. There could be an association between this 
      polymorphism and T2DM, whilst the polymorphism may protect diabetic patients from 
      having CVD. However, odds ratio is not statistically significant in both cases. 
      CONCLUSION: Our study supports the previous researches' results that Thr715Pro is 
      neither influencing the sP-selectin level nor the risk of CVD in T2DM patients.
CI  - (c) 2023 The Author(s).
FAU - Alzahrani, Faisal M
AU  - Alzahrani FM
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University. P.O.Box 1982, Dammam 31441, Saudi Arabia.
FAU - Alhassan, Jinan A
AU  - Alhassan JA
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University. P.O.Box 1982, Dammam 31441, Saudi Arabia.
FAU - Alshehri, Abdullah M
AU  - Alshehri AM
AD  - Department of Medicine, College of Medicine, Imam Abdulrahman Bin Faisal 
      University, P.O.Box 1982, Dammam 31441, Saudi Arabia.
FAU - Farooqi, Faraz A
AU  - Farooqi FA
AD  - College of dentistry, Imam Abdulrahman Bin Faisal University, P.O.Box 1982, 
      Dammam 31441, Saudi Arabia.
FAU - Aldossary, Maryam A
AU  - Aldossary MA
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University. P.O.Box 1982, Dammam 31441, Saudi Arabia.
FAU - Abdelghany, Magdy K
AU  - Abdelghany MK
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University. P.O.Box 1982, Dammam 31441, Saudi Arabia.
FAU - Ibrahim, Hafiz
AU  - Ibrahim H
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University. P.O.Box 1982, Dammam 31441, Saudi Arabia.
FAU - El-Masry, Omar S
AU  - El-Masry OS
AD  - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 
      Imam Abdulrahman Bin Faisal University. P.O.Box 1982, Dammam 31441, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20230202
PL  - Saudi Arabia
TA  - Saudi J Biol Sci
JT  - Saudi journal of biological sciences
JID - 101543796
PMC - PMC9944555
OTO - NOTNLM
OT  - ACE-I, Angiotensin-converting enzyme inhibitors
OT  - ARB, Angiotensin II receptor blockers
OT  - BMI, Body-mass index
OT  - CAM, Cell adhesion molecule
OT  - CCB, Calcium channel blockers
OT  - CVD, Cardiovascular disease
OT  - Cardiovascular disease
OT  - DM, Diabetes mellitus
OT  - ELISA, Enzyme-linked immunosorbent assay
OT  - Gp1balpha, Platelet glycoprotein 1b-alpha
OT  - IDF, International Diabetes Federation
OT  - IR, Insulin resistance
OT  - PMN, Polymorphonuclear leukocytes
OT  - PSGL-1, P-selectin glycoprotein ligand-1
OT  - SELP, P-selectin gene
OT  - T2DM, Type 2 diabetes mellitus
OT  - Thr715Pro polymorphism
OT  - Type 2 diabetes
OT  - WPb, Weibel-Palade Bodies
OT  - pP-selectin, Platelet P-selectin
OT  - sP-selectin
OT  - sP-selectin, Soluble P-selectin
OT  - vWF, Von-Willebrand factor
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/02/28 06:00
MHDA- 2023/02/28 06:01
PMCR- 2023/02/02
CRDT- 2023/02/27 04:30
PHST- 2022/10/03 00:00 [received]
PHST- 2023/01/03 00:00 [revised]
PHST- 2023/01/29 00:00 [accepted]
PHST- 2023/02/27 04:30 [entrez]
PHST- 2023/02/28 06:00 [pubmed]
PHST- 2023/02/28 06:01 [medline]
PHST- 2023/02/02 00:00 [pmc-release]
AID - S1319-562X(23)00024-4 [pii]
AID - 103579 [pii]
AID - 10.1016/j.sjbs.2023.103579 [doi]
PST - ppublish
SO  - Saudi J Biol Sci. 2023 Mar;30(3):103579. doi: 10.1016/j.sjbs.2023.103579. Epub 
      2023 Feb 2.